Genomic "dactyloscopy" with the use of bacteriophage M13 as a DNA probe (the expertise of material evidence and personal identification)

In this article the authors give a scientific evaluation of genetic dactyloscopy method in which the sites of human chromosomal DNA, possessing structural polymorphism, act as genetic markers. Technology of genome "dactyloscopy" including both the series of standard conventional methods and new methods is presented. The method is highly sensitive and requires small amounts of material for investigation. A practical case is described when genome "dactyloscopy" gave positive results which led to a conclusion on suspect's involvement in the crime.     

Laboratory evidence of unsuspected parental consanguinity among cases of disputed paternity

A search was conducted to find evidence of possible incestuous unions between the biologic parents of children involved in 2500 paternity cases. Suspicion was raised when either (1) a mother and her child possessed identical HLA phenotypes, or (2) the child appeared to be possibly homozygous for one maternal haplotype (i.e., one of the child's HLA haplotypes was a blank). These mother-child HLA-haplotype dualisms (MHDs) occurred in 5% of all cases. Frequency of exclusion of the accused men in cases demonstrating MHD, was compared with the remaining paternity cases.No significant difference was found in overall exclusion rates between MHD cases and controls when exclusion produced by HLA and red cell antigen systems were observed. However, there was a greater rate of exclusion in MHD cases when comparing exclusions produced by red cell antigen systems regardless of whether HLA tests excluded paternity (p < 0.025). MHD cases involving teenaged mothers differed from control cases in frequency of exclusion of paternity only on the basis of red cell antigen phenotyping (p < 0.005).The HLA system's usefulness in paternity testing is diminished when there is MHD; multiple, independently-inherited systems are relatively more useful in these circumstances.The search method detects only half of potential incest cases; proof of incest requires more extensive testing for homozygosity among other polymorphisms. Since calculations of likelihood of paternity are inappropriate in cases involving close consanguinity, detection and follow up studies are important.Data suggest that one-fifth of MHD cases may involve first degree consanguinity and that the incest rate among paternity cases may be as high as 2%.     

The primary investigation of material evidence and the evaluation of its suitability for expert biological identification by genomic "dactyloscopy"

Use of DNA "fingerprinting" method for biological analysis of material evidences exhibit new potentials for making concrete expert conclusions. But such expertise is time consuming and difficult to perform that's why it is important to evaluate fitness of expert material for DNA fingerprinting at early stages of object investigation. Method of preliminary treatment of objects sent for DNA fingerprint expertise in case of sexual assault is suggested and tested. This method allows one to evaluate objects easily from the standpoint of possibility to perform such investigation.     

The use of genomic "dactyloscopy" for the diagnosis of monozygotic twins

Possibilities of gene identification expertise for the solution of problem on mono- or dizygotic origin of twins were demonstrated. It was shown that DNA printing method makes it possible to differentiate mono- and dizygotic twins in complicated cases.     

Utility of HLA and six erythrocyte antigen systems in excluding paternity among 500 disputed cases

Six erythrocyte antigen systems and the HLA system were evaluated to establish their practical value in 500 cases of disputed paternity. The actual results were very close to predicted values. HLA testing is expected to detect 92% and red cell testing is expected to detect 67% of men falsely accused in paternity suits. The findings of this study show that HLA detected 94% and red cell testing detected 69% of 107 men falsely accused in 500 paternity cases. In order of sensitivity, Rh, MNSs, and ABO were the most useful erythrocyte marker systems. There were six out of 107 cases in which exclusions would have been undetected if red cell typing had not been performed. Five of the six cases involved "common" HLA haplotypes.     

DNA profile evidence in complex disputed paternity cases: The analysis of 300 real cases

In disputed paternity cases where the putative father is unavailable DNA from one or more of his relatives could be used. However, interpreting results is often difficult, because of the partial information regarding the parental genotype obtained from his relatives. We analyzed results obtained in 300 real paternity cases performed through close relatives of the real father (sib, half-sibs, one grandparent and/or uncle). DNA was typed with PowerPlex (Promega) and the LR estimated with the Software BDGen. As expected the higher LR values were achieved with sibs and half-sibs (in such cases where his/her mother was available for testing). The LR values were tight related to the number of uninformative loci, which varied between 0 and 13. In 10% of the reviewed cases, 10 or more non-informative loci were observed; all of them associated LR values below 0.01. Thus, providing evidence in favor of no relatedness.     

Introduction of a standardized "paternity index" for the statistical evaluation of blood group findings in paternity testing

The introduction of a standardized paternity index (PI) for the statistical evaluation of blood group findings in cases of disputed paternity is proposed and explained. By using the PI $\text{X}\text{Y}$ as parameter, it is not necessary to give the information of the probability of paternity in percent. The PI includes the full information of the blood group findings. In addition to that, by using the suggested standardization based on the probabilities of error according to Schulte Mönting and Walter the test volume is also taken into account. The interpretation of the mathematical result is given by verbal predicates, the limitations of which are orientated by the verbal predicates for the probabilities of error according to Schulte Mönting and Walter, published by us elsewhere. Besides the essential fact that the test volume is taken into account, the most important advantage of this procedure is that the mathematical result is involved in the court decision only by the PI (which is free of any valuation) and its verbal predicate and not by sometimes relatively high percentages, which may be misunderstood by laymen.     

The palatal ruga pattern in possible paternity determination

The use of a genetic marker in paternity determination has been successful with the ABO blood group system but unsuccessful in dermatoglyphics and palatal rugae because the genetic mechanism is polygenic. The palatal rugae of 17 families (34 parents and 49 children) were classified and recorded, and the data used to construct a statistical analysis system (SAS) cluster map. A positive result would have meant a father clustering with all his children at Level 83, where, in fact, the best result achieved was at Level 5. The best cluster occurred at Level 82 between the ten-year-old boy of Family 7 and the eleven-year-old girl of Family 3. It is thus clear that the palatal rugae cannot be used in a practical procedure to determine paternity.     

Biostatistical evaluation of evidence from continuous allele frequency distribution deoxyribonucleic acid (DNA) probes in reference to disputed paternity and identity

We present a development and discussion of the biostatistical evaluation of deoxyribonucleic acid (DNA) probe evidence in forensic science cases of disputed paternity and identity. We restrict ourselves to single-locus codominant systems (highly analogous to more conventional systems) which have the apparently novel complication of an experimentally continuous allele frequency distribution. This complication necessitates reformulations of standard biostatistical summaries of the evidence (the paternity index (PI) and the phenotype frequency, respectively). These reformulations, rather than representing a unique case, have applicability to the evaluation of evidence obtained in standard genetic systems now in widespread use.     

1例五四式手枪伤人的法医学鉴定及实验研究

五四式手枪伤人案件的法医学鉴定通常较为顺利，但在一些特殊情况下，其射入口和射击距离也引起法医学上的讨论。现将1例五四式手枪致人死亡案例的鉴定过程及所做的相应实验报告如下：案情一司法警察团公务与一群村民发生冲突时击发五四式手枪，致一人死亡。当地法医第2天对死者进行尸表及局部解剖检验认为：死者左臀部创口为射入口；左下腹部创口为射出口，创口周围的点状表皮剥脱为昆虫叮咬所致；射击距离为50cm以远。与调查情况出入较大，遂报上级法医复核鉴定，第3天进行复核检验。复核检验情况：枪击现场为一开阔的土坡上，已遭严重破…     

1例五四式手枪伤人的法医学鉴定及实验研究

五四式手枪伤人案件的法医学鉴定通常较为顺利,但在一些特殊情况下,其射入口和射击距离也引起法医学上的讨论.现将1例五四式手枪致人死亡案例的鉴定过程及所做的相应实验报告如下:     

The determination of head circumference and hat size in the expertise of the human skull

Based on the results of examinations of craniocephalic characteristics in 47 corpses of adults of both sexes, a method for identifying the head circumference and hat size has been developed for expert evaluation of skeletonized rudiments. Hats known to belong to the lost subjects are proposed to be used as the reference samples. The author emphasizes the importance of the time when the hat was made and its position on the head.     

Application of probability of paternity calculations to an alleged incestuous relationship

An alleged case of incest between half siblings has been examined by standard blood grouping and human leukocyte antigen (HLA) serology. The data were analyzed statistically using single and joint possibilities of paternity. The existence of the alleged relationship between the two parties in question is quite probable.     

The detection of beta-cypermethrin during forensic chemical expertise of biological materials

The optimal conditions for the isolation of beta-cypermethrin from biological materials by means of dioxane extraction have been developed. The possibility to purify beta-cypermethrin from endogenous impurities is demonstrated by combination of liquid-liquid extraction and chromatography through a silica gel column (L 40/100 mcm). It is shown that identification and quantitative determination of beta-cypermethrin extracted from the cadaveric liver tissues are possible using such techniques as thin-layer chromatography, electron spectrometry, and chromato-mass spectrometry. A method for forensic chemical expertise of the presence of beta-cypermethrin in biological materials is described.     

Colorimetric determination of endrin in biological materials

A simple colorimetric method for the determination of endrin in formulations has been reported. In the present communication, the application of the method to biological specimens is discussed. The method is sensitive and obeys Beer's law for the concentration range 4 – 40 μg/ml, with 99 ± 3% recovery of endrin added (in vitro) to the biological specimen.