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Comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC x GC-TOFMS) for drug screening and confirmation 总被引:1,自引:0,他引:1
Comprehensive two-dimensional gas chromatography (GC x GC) is applied to analysis of drug standard mixtures containing 78 drugs of interest in forensic samples. For this study, underivatised drugs were employed. While several of the drugs were not detected at the low concentrations employed in the samples, most could be satisfactorily assigned their first and second dimension retentions in the GC x GC retention plane. For this study, time-of-flight mass spectrometry (TOFMS) detection was used. The enhanced separation possible in GC x GC is demonstrated, and typical linearity and apparatus precision are shown for tramadol, diazepam, olanzapine and desipramine using selected qualifier ions. Mass spectral library search quality for the detection of drugs in a selection of authentic forensic cases, along with retention position in the 2D retention plane, is used to support positive identification of the presence of the drugs. The analysis of 'difficult' drugs paracetamol and phenytoin is shown to produce anomalous chromatographic peak shape in the 2D plane, whereas most drugs gave acceptable peak shapes. The GC x GC technique was applied to screening drugs in forensic samples, with either flame ionisation (FID) or TOFMS detection, and compared favourably with conventional single column GC-MS analysis when tested for diazepam in an authentic forensic study. 相似文献
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Here we examine the current state of palynology in the field of forensic science. Forensic palynology is discussed with reference to other forensic disciplines to help understand what is required for its progress. Emerging developments are also discussed. Palynomorphs potentially deliver excellent trace evidence, fulfilling the requirements relating to the transfer, persistence, and detection of such evidence. Palynological evidence can provide very powerful investigative and associative evidence. Despite this, the application of palynology to forensic science has had mixed success. There are many anecdotal stories where pollen evidence has had spectacular successes. But it is extremely underutilized in most countries because it is labor-intensive and requires considerable expertise and experience, there is a lack of control over sample collection and inadequate resourcing and funding, and its crime-solving power is not well known. Palynology has been applied to forensic problems in an unstructured way, resulting in a lack of formalized discussion of the underlying principles. As there is renewed questioning of the acceptability of most evidence types in the current legal environment, there is a need for the establishment of palynological evidence through validation-type studies and experimentation, and the implementation of independent proficiency testing. 相似文献
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E M Suzuki 《Journal of forensic sciences》1992,37(2):467-487
Although the diamond anvil cell (DAC) has been used in many forensic science laboratories for the analysis of trace evidence, few applications of this technique for the analysis of controlled substances have been reported. This may be due to both an unfamiliarity on the part of forensic drug chemists with this accessory and the nature and quality of spectra that result from use of a DAC on a dispersive instrument. Along with low energy throughput, which results in relatively high noise levels, strong broad diamond absorptions occur. With the use of a Fourier transform infrared instrument, these do not present a problem and nanogram quantities of materials can be analyzed when the DAC is used with an infrared microscope. Since single crystals can be sampled with the DAC, simple physical separations (involving particle-picking) can be used in certain cases to isolate drugs from particulate mixtures for infrared analysis. This method is especially useful for some "difficult" mixtures and residues, and several examples of such analyses involving samples of forensic science interest are presented. 相似文献
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This study focuses on the trust that potential jurors have in unsubstantiated evidence and the implications of such trust for legal decision-making. We examined whether participants’ motivation to think deeply (‘need for cognition,’ NC) and belief in science moderated their trust in potentially fallible detection dog evidence when selecting a verdict in a trial scenario. A detection dog twice indicated the presence of drugs in the scenario, yet no drugs were actually found. Those who chose a guilty verdict without drugs present featured stronger beliefs in detection dog evidence. They were also more confident that a dog alert indicated the presence of drugs, even though the scientific literature actually shows that detection dog evidence is subject to biases and other challenges to reliability. Our findings indicate that an unsubstantiated belief and trust in detection dog evidence may negatively influence juror decision-making, which may, in turn, pose consequences for fairness and justice. Participants believed that detection dogs provide powerful and reliable evidence, and these beliefs were clearly associated with stronger beliefs in science. These findings, therefore, raise serious concerns about jurors’ indiscriminate trust in forensic evidence, be it detection dog evidence or other lines of evidence presented in court. 相似文献
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In recent months, there has been increased reporting of seized drug and toxicology cases containing rodenticides, the active ingredient in rat poisons. Seeing as rodenticides are not scheduled substances, they are not commonly screened for in seized drug analysis. This work investigates the use of TD‐DART‐MS for the simultaneous detection of rodenticides and drugs. Six rodenticides were evaluated, an optimal method was established, and limits of detection in the tens of nanograms were calculated. Additional studies highlight that detection at less than 1% by weight in mixtures with AB‐FUBINACA, cocaine, heroin, or methamphetamine was possible. This work presents an optimized method for detection of these compounds, allowing for the simultaneous detection of drugs and rodenticides, providing drug chemists with a tool for rapid identification of these compounds for forensic or public health purposes. 相似文献
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《Science & justice》2014,54(2):164-169
Raman microscopy is a useful tool for the analysis of drug particles collected with adhesive tapes. In this work, first, the spectra of thirty drugs of abuse, degradation products, metabolites, and common cutting agent standards were recorded and the Raman bands observed were summarized providing the forensic analyst useful information for the identification of drug evidence. Then, the collection of different drug particles by a fingerprint lifting tape commonly used to remove and store fingerprints and fibers, and a white and green packaging tape, followed by the subsequent identification of the drugs by confocal Raman spectroscopy was performed. The particles were analyzed on top of the tapes, trapped between glass slides and the tapes, trapped in the tape folded over itself in the case of the transparent tape, and after folding and unfolding the tape in the case of the colored tape. The results obtained by the different approaches show that both tapes did not compromise the drugs spectra. However, the use of transparent tape is preferred because this tape allows the previous visual detection of the particles. Finally, several drug and sugar particles were spread over a clean table and inside a pocket, and the particles were collected with transparent tape and then properly identified. Although good results were obtained in both cases, the amount of fibers and other substances present in the collection area made the previous detection of the particles difficult and increases the analysis time. 相似文献
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The detection of semen in forensic science is essential in cases of sexual assault but can be problematic in the absence of spermatozoa. Choline is known to occur in high concentrations in seminal fluid and the Florence Iodine test for its detection has been used in forensic science for many years, however very little is documented regarding its sensitivity and specificity in forensic casework. This paper describes the optimisation of the choline Florence Iodine test (FI) and investigates the sensitivity and specificity of the test against different body fluids, food and drink substances, cleaning products and laboratory chemicals. Comparative testing against Acid Phosphatase (AP) and Prostate Specific Antigen (PSA Seratec®) tests is described and shows that the FI test has greater specificity than the PSA test which cross reacts with a number of body fluids. 相似文献
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《Science & justice》2023,63(1):83-89
The determination of fiber dyes is important in forensic investigations. Although a variety of fiber dyes detection methods have been established, the sensitive and accurate determination of trace fiber dyes remains a challenge due to the possible interferences caused by complex environmental matrix and various fiber additives. Orbitrap mass spectrometry (Orbitrap MS) is a type of high-resolution mass spectrometry with high qualitative accuracy and detection sensitivity which highly meet the identification requirements of fiber dyes in real cases. However, the application of Orbitrap MS in fiber dye analysis is limited. In this regard, this study used polyester fiber, which is the most commonly-found fiber in forensic cases, as a model and established a UHPLC–Orbitrap MS method to analyze disperse dyes on polyester fibers. Using the optimized UHPLC–Orbitrap MS method, nine disperse dyes were accurately identified and well separated, and the limits of detection ranged between 0.1 ng/mL and 5.0 ng/mL. The developed method was applied to analyze actual fiber samples, and dyes from single fibers of 1 mm in length could be accurately detected. The established method is sensitive, accurate, and demonstrates good application prospects. 相似文献
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Amy L. Patton B.S. Krishna C. Chimalakonda Ph.D. Cindy L. Moran B.S. Keith R. McCain Pharm.D. Anna Radominska‐Pandya Ph.D. Laura P. James M.D. Charles Kokes M.D. Jeffery H. Moran Ph.D. 《Journal of forensic sciences》2013,58(6):1676-1680
Limited forensic and clinical experience and the lack of confirmatory testing strategies for synthetic cannabinoids (SC) prevent adequate characterization of SC toxicity and the potential impact on public health. A statewide surveillance system identified a fatality involving a 23‐year‐old man found with a large stab wound to the neck following use of a SC product suspected of containing AM2201. Analytical testing for common SCs, SC metabolites, routine drugs of abuse, and over‐the‐counter medications was performed on heart blood obtained at autopsy. Additionally, assays were performed on the SC raw material and drug paraphernalia found on the decedent. High concentrations of AM2201 were detected in all samples. AM2201 metabolites were detected in postmortem blood. Other than a trace amount of JWH‐073 found in smoke residue, no other substances were detected. Psychiatric complications including self‐induced, lethal trauma can occur after the use of SC products. 相似文献
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Heroin street doses are complex mixtures commonly analyzed in forensic laboratories. Identification of the illicit substance in these street doses is among the primary analytical tasks of a forensic laboratory. We demonstrate that the one-dimensional ID-TOCSY NMR experiment permits identification of heroin in standard mixtures containing up to ten or more different components. This method produces an easily-identified and effective "fingerprint" for heroin within a mixture of other substances. The method has been successfully tested as a tool for identification of heroin in street doses from police casework in Israel. This NMR technique is robust and quick (a measurement can be carried out in 10-15 min), and it does not require any preliminary physical or chemical treatments of the sample to be examined, due to the effective spectroscopic "filtering" of the interfering components. The ID-TOCSY NMR method can potentially be used in combination with additional analytical methods as a routine tool in forensic laboratories to positively identify heroin for court purposes. 相似文献
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《Science & justice》2022,62(6):758-767
Incorporating a simulated crime scene into one’s pedagogy in forensic science undergraduate courses allows students the opportunity to experience a realistic scenario while demonstrating their knowledge and enhancing their critical thinking skills. The purpose of this paper is to examine an active learning approach to using simulated crime scenes to provide an impactful learning experience for students. While potentially challenging for the instructor, constructing a crime scene scenario can provide students with hands-on practical experience while helping to dispel forensic science misconceptions. Through many years of creating crime scenes, best practices for using such high impact activities in forensic science courses are described in detail, including preparatory exercises that culminate in the final crime scene scenario, considerations in preparing and constructing a crime scene activity, and supervising and assessing students once the activity begins. Three major challenges to implementing active learning exercises such as simulated crime scenes in undergraduate forensic science programs include high workload for instructors, limited supplies, and lack of suitable facilities. Workload solutions include instructors and departments considering the cost and benefit of course releases to improve curriculum and student enrollment. Supplies and facilities solutions involve innovation in reuse and repurposing of supplies, and instructor flexibility in using classroom and outdoor spaces. 相似文献
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Weyermann C Mimoune Y Anglada F Massonnet G Esseiva P Buzzini P 《Forensic science international》2011,209(1-3):21-28
A transportable Raman spectrometer was tested for the detection of illicit drugs seized during border controls. In a first step, the analysis methodology was optimized using reference substances such as diacetylmorphine (heroin), cocaine and amphetamine (as powder or liquid forms). Adequate focalisation distance and times of analysis, influence of daylight and artificial light sources, repeatability and limits of detection were studied. In a second step, the applications and limitations of the technique to detect the illicit substances in different mixtures and containers were evaluated. Transportable Raman spectroscopy was found to be adequate for a rapid screen of liquids and powders for the detection and identification of controlled substances. Additionally, it had the advantage over other portable techniques, such as ion mobility spectrometry, of being non-destructive and capable of rapid analysis of large quantities of substances through containers such as plastic bags and glass bottles. 相似文献
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Kovalenko AE Kardonskiĭ DA Eganov AA Grishin DA Salomatin EM 《Sudebno-meditsinskaia ekspertiza》2007,50(2):28-32
A method of analysis of basic narcotic drugs in urine is described. It consists in isolation by solid phase extraction, derivation and identification by gas chromatography with mass-selective detector. The approaches applied in the above method can be used for analysis of narcotic drugs undetectable by preliminary investigations as well as for urine examination for pharmacological or other biologically active substances. 相似文献
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A 79-year-old woman, with Parkinson's disease treated with cabergoline, was admitted to a hospital due to jaundice and weakness. She was found confused, absent minded, and died after 2 weeks. Autopsy showed an extrahepatic bile duct adenocarcinoma with spread to the gall bladder, the liver, and regional lymphnodes. While cleaning the hospital bed after her death, the nurses found several tablets hidden in the bed. Biological samples obtained at the autopsy were screened for common drugs and narcotics. Several drugs such as buprenorphine, codeine, paracetamol, and propranolol were detected in the blood at therapeutic levels. A method to determine cabergoline in whole blood and other forensic matrices was developed, and further investigations determined cabergoline concentrations in whole blood and liver tissue of 94 and 3100 microg/kg, respectively. The blood concentration was 100 times above the therapeutic level reported on cabergoline in plasma and in combination with her symptoms, suggest she took a fatal overdose of cabergoline. 相似文献
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