韩国1998-2001行政改革的基本经验

此次韩国行政改革在政府机构、管理制度、行政文化等相关层面施行了平衡到位相互协调的改革举措 ;改革的切入点是以职能转变为中心的政府机构重组、开放型人事制度的推开、高级公务员年薪制的试行、责任运营制度的建立、绩效管理制度的试验 ,顾客宪章制度的引进 ,以及部分公共管理事务外部委托制的探索 ;其基本经验是 ,机构改革的效度有赖于行政系统内相关要素的全面优化 ,行政改革的效度则有赖于整体改革环境的优化 ,民间专家参与是促进政府改革的重要资源 ,政府间行政架构模式决定了地方机构改革的成效。     

Family Context and Childhood Adjustment Associated with Intrafamilial Unwanted Sexual Attention

Unwanted sexual attention (UWSA) encompasses unsolicited verbal comments, gestures, stares, and other noncontact behaviors made regarding one's sexuality and physical appearance. The present study examined the correlates and impact of such UWSA perpetrated toward girls by family members. The first objective of the study was to explore whether family climate is associated with intrafamilial UWSA. This included looking at three types of dysfunctional parenting styles (unaffectionate, unavailable, and patriarchal) as well as exploring the co-occurrence of UWSA by adult and child relatives. The second objective was to then measure the impact of such intrafamilial UWSA on childhood emotional health, after controlling for parenting style and the experience of more traditional forms of child sexual abuse (CSA). Of the 296 university women (mean age = 19) who participated in the study, 70% (N = 206) reported UWSA from a family member prior to age 18. Whereas each dysfunctional parenting style was related to presence of UWSA, a simultaneous multiple regression analysis indicated that two of the parenting styles, patriarchal and parental unavailability, also predicted frequency of nonphysical UWSA within the family. Presence of UWSA from a child relative was significantly correlated with presence of UWSA from an adult relative. Additionally, a hierarchical multiple regression, entering the three parenting styles simultaneously in the first step, childhood sexual abuse in the second step, and familial UWSA in the last step, indicated that the frequency of UWSA by family members significantly predicted poorer childhood mental health when controlling for the other variables. Overall, results indicate that while the specific parenting styles which co-occur with UWSA may be detrimental in their own right, the impact of UWSA on girls appears to be above and beyond that of either parenting styles or CSA. Clinical implications are discussed.     

欧盟“刑事司法区”述评

自建设欧盟"刑事司法区"理念提出以来,欧盟成员国致力于加强彼此之间的刑事合作。在欧盟宪法性条约的导引下,欧盟及其成员国通过制定与实施一系列重点行动计划及项目,在引渡、刑事互助、刑事判决的相互承认等方面取得了突破,一个欧盟范围内的"刑事司法区"正在形成。     

堆型艾美球虫广东株3-1E基因的克隆与序列分析

根据GenBank中登录的堆型艾美球虫31E基因序列,设计了3条引物,以广东株堆型艾美球虫裂殖子总RNA为模板,利用反转录聚合酶链反应(RTPCR)扩增获得了31E基因部分片段,将这一片段克隆至pGEMTEasy载体中,经PCR、限制性内切酶分析和克隆片段的序列测定、比较,证实了克隆片段的可靠性。序列比较发现,所克隆的基因片段与Eimeriaacervulina美国株(US)、E.acervulinaQH株31EcDNA的核苷酸同源性分别为99.0%和99.2%,推导氨基酸的同源性分别为98.2%和97.6%。     

自行车交通事故推断交通方式1例

自行车交通事故中为明确责任大小,往往需要先明确横穿公路者交通方式是骑行还是推行,由于一些客观条件限制,证人证言、现场痕迹等证据不能明确该关键点,这就需要从尸体上寻找相关证据。笔者曾根据现场车辆碰撞痕迹及尸检所见,运用法医学知识,判断死者交通方式1例,现报道如下。案例资料死者吴某,女,31岁。2003年4月8日11时15分,与其丈夫一前一后骑自行车,当到达一划有人行横道线路口时,吴某先横穿道路,被一辆小货车碰撞,经抢救无效死亡。由于证人证言出现矛盾,推行还是骑行无法确定,如骑行,应负次要或同等责任,如推行,则无责任。现场勘查:自…     

边缘无浆体MSP5蛋白基因的克隆及原核表达

参照已发表的边缘无浆体主要表面蛋白5(MSP5)基因的核苷酸序列,设计了1对特异性引物,以边缘无浆体基因组DNA为模板,采用PCR技术扩增获得了MSP5蛋白基因。将其克隆到pGEM-T Easy载体,并进行测序分析。结果表明,克隆的MSP5基因与GenBank上登录的Florida株MSP5蛋白基因的序列同源性达98.6%,编码氨基酸的同源性为99.0%,并且该序列包含有完整的开放阅读框,大小为633 bp。将该基因亚克隆入原核表达载体pGEX-4T-1,构建了重组原核表达载体。将其转化到DH5α宿主菌中,用IPTG进行诱导表达,实现了融合表达,表达产物的分子质量为45 ku。Western-blot分析表明,此表达产物能够被抗边缘无浆体阳性血清所识别。     

做好火灾保险推动火灾预防

火灾已经成为最经常、最普遍威胁公共安全、经济建设和社会稳定的一种灾害。因此,做好火灾保险与火灾预防是经济和社会发展的必然需求。     

现代性重建进程中的法治变革趋向

随着现代性的时代发展,现代性反思和重建已成为一个必须直面的问题,正是在这一境遇下,现代法治也开始发生了重要变革,形成了法律多元主义的规则秩序、互动平衡的功能取向、反思回应的程序主义诉求等发展走向。因此,我们应立足于现代性反思和重建的时代关怀,积极推进中国的法治进程。     

细菌分型的分子生物学技术研究进展

回顾了传统细菌分型方法的重要作用,并重点介绍了近年发展得比较成熟的脉冲凝胶电泳(PF-GE)、低频限制性切割位点聚合酶链式反应(IRS-PCR)、随意扩增多态性DNA(RAPD)、扩增片段长度多态性(AFLP)、多位点酶电泳(MLEE)和多位点测序分型(MLST)等6种用于细菌分型的分子生物学技术的原理、特点以及应用情况,指出了其他细菌分型技术,如ARDRA、SSCP、DGGE、TGGEI、S等的不足,对基因芯片技术和实时PCR在细菌分型上的应用潜力进行了展望。     

Justice for All: Putting Victims at the Heart of Criminal Justice?

This article considers the claim in the government's White Paper, Justice for All , to put victims and witnesses at the heart of the criminal justice system and argues that there is an unresolved tension within the paper between instrumentalist crime control concerns and intrinsic concerns for the rights of victims and witnesses. It is argued that many of the proposals now contained in the latest Criminal Justice Bill are so preoccupied with rebalancing the system away from offenders that they risk doing injustice to defendants with little tangible benefit to victims and witnesses in terms of rights and remedies.