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Isolation of the DNA minisatellite probe MZ 1.3 and its application to DNA 'fingerprinting' analysis 总被引:1,自引:0,他引:1
U Schacker P M Schneider B Holtkamp E Bohnke R Fimmers H H Sonneborn C Rittner 《Forensic science international》1990,44(2-3):209-224
A minisatellite probe, MZ 1.3, detecting hypervariable fragment patterns was isolated from a human genomic library. A repetitive sequence of 27 bp length was identified which is contained in the probe approx. 40 times. The MZ 1.3 repeat shows variable homology of 53-73% to the repetitive sequence of the protein III gene of the bacteriophage M13 genome. Polymorphic restriction fragment patterns were found with MZ 1.3 using the enzymes Hinf I, BstN I, Hae III, Mbo I, PstI/Pvu II, and Rsa I. An average of 18 polymorphic fragments was observed using Hinf I as enzyme. The band sharing frequency after Hinf I digestion among unrelated individuals was determined to be 23.8 +/- 7.2%. An example for the application of MZ 1.3 to paternity testing in an incest case is given. The probe can be used with radioactive or non-radioactive detection systems. An approach is presented to compare polymorphic fragment patterns from individuals obtained by independent gel runs on the basis of relative band positions (RBP) and calculated in a computerized analysis. 相似文献
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We report a case of rapid death from thrombotic thrombocytopaenic purpura (TTP) in a young pregnant lady who developed full blown symptoms soon after caesarean section. Extensive intramyocardial confluent haemorrhages and widespread microthrombi in heart, brain, adrenals and kidney were found at autopsy. Thrombotic thrombocytopaenic purpura is an uncommon condition, which carries a high fatality rate if untreated. Awareness of this syndrome together with its high risk of sudden death underlines the importance of rapid diagnosis and treatment. 相似文献
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This article argues that Korea should not completely liberalize its rice market. The argument begins from the issue of food-security policy to the contradiction between self-sufficiency and the comparative advantage of the importance of agricultural protection, in light of advanced industrialized nations’ heavy subsidies of their agricultural commodities, including rice, and implicit and explicit trade barriers on Korean exports. The argument emphasizes the importance of self-sufficiency policy for rice in Korea, thus recommending not complete but partial liberalization of the agricultural market, as a condition for Korea’s own self-interest. The argument also emphasizes the importance of a diversified foreign and economic policy for Korea, and thus of forming future security relations with other nations besides the United States. 相似文献
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Carol U. Lindquist Lillian E. Sass Denise Bottomley Suzanne M. Katin Jennifer D. Maddox Rose M. Ordonez Craig N. Teofilo 《Journal of family violence》1997,12(1):75-83
Current research suggests that a husband's substance abuse is correlated with severity of physical abuse and the woman's decision to leave a violent situation. Often, only the battered woman's report of abuse is available. This study compares women's reports of their partners' substance use/abuse with their partners' report using a brief measure of polydrug and alcohol abuse, the Substance Abuse Subtle Screening Inventory (SASSI) and the Conflict Tactics Scale (CTS). Data were analyzed for 25 recovery couples and 25 nonrecovery couples. The correlations for all 50 couples between the male's reported use and the female's report of her partner's use on the SASSI and the CTS were significant on all but one of the CTS scales. They ranged on SASSI from .73 to .33 and from .31 to .06 on the CTS. This suggests that the SASSI and possibly the CTS could be used as valuable tools for assessing women's reports of their partner's substance use/abuse. 相似文献
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U.S. National Institutes of Health 《Federal register》1997,62(66):16596-16602