中华鳖全血呼吸爆发化学发光检测方法的建立

为进一步研究中华鳖的先天免疫能力及其机理,以酵母多糖为吞噬底物,通过对鲁米诺浓度、全血稀释度和检测温度等条件的优化,建立了中华鳖全血呼吸爆发的化学发光检测方法,并用此方法对放置不同时间的全血和稀释血液进行了呼吸爆发检测效果的比较。结果表明,鲁米诺浓度为1×10-4mol/L,检测温度为31.5℃时,以0.4~0.8μL/mL的中华鳖全血进行检测可获得理想效果。保存6 d的全血仍可检测到呼吸爆发,稀释全血放置30 h后不再产生呼吸爆发。     

环境权:一种新兴的现代人权

人权是最基本的权利,环境权来自人权。生存权位于人权的核心,而环境权作为一项基本人权是不可剥夺、不可放弃和替代的权利。如果没有环境权,传统人权体系中的生存权就不可能得到完善的保障。环境权源于生存权,但超越了生存权,因此它是一种新兴的现代人权。     

加入WTO后的企业领导者角色定位

中国已加入WTO ,企业迎来了一次更大的开放和竞争。企业领导者要想使自己所领导的企业在激烈的国际竞争中立于不败之地 ,就要正确认识加入WTO给企业带来的机遇和挑战 ,熟悉和学会运用WTO的有关规则 ,并采取积极的应对措施     

胶体金标记苯丙胺单克隆抗体免疫试剂盒研究

目的建立一种准确、快速、简便的检测尿液中苯丙胺(AMP)的胶体金免疫层析技术。方法采用柠檬酸三钠还原法制备胶体金颗粒,标记抗AMP单抗,将AMP—BSA抗原固相于硝酸纤维素膜上,制备胶体金免疫层析测试条。通过尿液、血液和唾液中的可能存在的苯丙胺成分与测试条上的苯丙胺-BSA完全抗原竞争结合有限的单抗结合位点,来判定检测结果。结果用ICT法和GC/MS检测217份尿样,本法检测阈值为1000ng/mL,特异性为99.17%,准确性为99.54%。结论ICT法检测尿液中的AMP特异性强,灵敏度高、简便快速、无需特殊仪器设备,具有广泛应用价值。     

Four Lessons for Developing Countries from the Trade Negotiations Over Access to Medicines

After the Agreement on the Trade-Related Aspects of Intellectual Property Rights (TRIPS) came into operation in 1995 developing countries have found themselves in a process of continual negotiation over intellectual property rights and access to medicines. These negotiations have taken place in the World Trade Organization and in the context of free trade agreements. The paper suggests that the only real win for developing countries has been the Doha Declaration on the TRIPS Agreement and Public Health in 2001. What have been the lessons for developing countries in a decade of negotiations over access to medicines? Drawing on themes of rule complexity and regulatory ritualism the paper discusses four key lessons for developing countries. It concludes by arguing that developing countries will do better if they adopt a networked governance approach to negotiation rather than continuing to rely on traditional coalition formation.     

论我国现行立法背景下的物权变动模式

物权的变动模式作为物权法的重要内容,一直是学界讨论的热点。如何正确认识我国现行立法背景下的物权变动模式,对于未来物权立法能否选择一个既与国际接轨又适合我国国情的物权变动模式,有着重要的意义。笔者认为,我国现行法规定的物权变动模式,虽非债权意思主义,但也绝不是学界通说的债权形式主义,而是一种特有的模式——物权意思主义。     

“The Spectacle of Fearsome Acts”: Crime in the Melting P(l)ot in <Emphasis Type="Italic">Gangs of New York</Emphasis>

This paper explores the multi-layered representations of violence and crime in the recent Hollywood film Gangs of New York [Scorcese (2003) Miramax]. We use our exploration of this film to suggest that popular culture, even through its most mainstream products, can be seen as a critical criminological space where alternative views of law, crime and the state are made available. Rather than understanding Hollywood movies simply as vehicles for disseminating conventional mores, we suggest that they can furnish critical (and complex) points of view on law and crime and that the project of a critical criminology can be strengthened by engaging more forthrightly with these ubiquitous cultural forms.     

四川乡镇选举方式改革的分类与反思

四川乡镇选举方式改革有着特定的背景,在乡镇选举方式改革实践中形成了两大类型,即间接选举方式改革和直接选举方式改革,都取得了积极成效,但同时也都存在着大众参与机制不足、制度设计不够完善等不足,需要引起注意。     

一个关于判决、调解和执行关系的实证研究

本文的目的是探讨判决和调解对执行的影响。对判决、调解和执行关系的考察主要侧重于判决和调解相比,哪一种方式结案的案件容易进入执行程序?换句话讲,同调解结案的案件相比,判决结案的案件是不是更容易进入法院的执行程序。拿到判决的当事人,是不是比拿到调解的当事人更容易申请法院强制执行?