番鸭细小病毒弱毒株VP2基因的分子克隆与序列测定

将番鸭细小病毒(MDPV)强毒Q株经番鸭胚连续传代,获得致弱株MDPV-26.根据已发表的MDPV的全基因序列,设计了1对引物LHMP7/LHMP8,同时在这2条引物中分别加入2种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点.应用PCR技术扩增了MDPV-26株的VP2基因片段.将扩增后的基因片段重组到pMD18-T质粒载体上,并对插入片段进行序列测定.测序结果表明,弱毒株MDPV-26与强毒株MDPV-Q的VP2基因序列相同率达99.7%,与国外分离株的同源性为98.3%,说明强、弱毒株的VP2基因序列变化不大.     

吞吴失策遗恨千古--浅析杜诗《八阵图》所咏诸葛亮的"遗恨"

杜甫《八阵图》诗所咏诸葛亮的“遗恨”, 隐括了三国时期蜀、吴联合抗魏的统一战线遭致破坏的历史事件及诸葛亮为此而生的千古遗恨。诸葛亮之所以事前不谏刘备东伐吴国,实因有其难言之隐。这也是诸葛亮抱恨终生的底蕴所在。     

当代世界文化格局、态势与中国文化发展战略

当代世界文化格局主要由中华、欧美、阿拉伯、印度、俄罗斯及东欧这五大文化区构成。其演变态势,一是互动加剧并趋于良性,二是欧美文化区中的强势国家对其他文化区有扩张、挤压、排斥和利用之举,三是五大文化区之间力量对比和各自内部处在此消彼长和新的分化组合之中,分别显现为“龙虎斗”、“亢龙有悔”、“潜龙勿用”、“见龙在田”。我们要认清自身的文化方位,增强使命感和文化自觉意识,大力将先进文化建设的战略部署落到实处。     

Tweets That Matter: Evidence From a Randomized Field Experiment in Japan

Although election campaigns are increasingly utilizing social media, only a few studies have investigated their effects experimentally. To fill this gap in the literature, we conducted a field experiment to examine the effects of a campaign that used Twitter during the 2013 House of Councillors election in Japan. The treatment was exposure to tweets from Tōru Hashimoto, the mayor of Osaka and co-leader of the Japan Restoration Party, who has the largest number of Twitter followers among Japanese politicians. Participants assigned to the treatment group followed Hashimoto and the two placebos, whereas those assigned to the control condition followed only the two placebos. They followed the politicians continuously for approximately one month. Pre- and posttreatment measures were collected using online surveys, and treatment compliance was continuously checked via Twitter application programming interface (API). Following Hashimoto on Twitter during the election campaign had a positive impact on feelings toward Hashimoto. This effect was not mediated by issue knowledge or the evaluation of Hashimoto’s personal traits, and no effects were observed on voting. These findings suggest that repeated exposure to a politician’s messages on Twitter may only result in a mere exposure effect, which nevertheless generates favorable overall attitudes about the politician.     

论党员监督权

党员享有监督权是党员进行党内民主监督的前提。党员监督权包括批评权、检举权和要求罢免权。党员监督权具有工具性、综合性、重合性等特征,具有评价、免疫、善事、防变等功能。保障党员有效行使监督权,对于反腐防变具有重要意义。     

我国省域循环经济发展的综合评价、类型分析与路径探讨

受资源环境、地理区位、经济发展、技术创新等综合因素影响,我国省域循环经济发展水平有较大差异。基于2005—2013年的数据,通过综合评价可将我国省域循环经济划分为领先型、挑战型、追赶型和后进型四大类型。不同类型地区循环经济发展的基础条件和存在问题各不相同,应分别采取创新驱动、制度突破、产业循环、政府引导的差异化提升路径,协调推进我国省域循环经济发展和生态文明建设。     

Opening Remarks at the Second Plenary Session of the 10~(th) Executive Council of CAFIU

Mr.Wang Jiarui,Minister of the International Department of CPC Central Committee,Vice-Presidents of CAFIU,Council Members of CAFIU,Colleagues and friends,On such a beautiful spring day,I am so glad to be here to attend the second plenary session of the 10th Executive Council of CAFIU.I would like to start by extending warm welcome to all of you,who have made time out of your busy schedule to be present today.I also want to thank you for your trust and support to me in my past two years as president of CAFIU.     

故国莺花 前朝风月——《金诗人王寂描写阜新的那些诗》连载之一

阜新地区明确而具体地被载八的史册是元朝修的《辽史》，这同周边城市如朝阳、北镇相比至少晚了一千多年。但是，阜新一经见诸载记就是个发达地区，其文明程度可能远超中原偏僻州县。原因简单，当初的阜新是靠内地移民发展起来的。契丹贵族对中原王朝频频用兵的直接目的就是掠夺财富，而俘获汉族人口是财富之大宗。     

Building A Platform for Understanding and Establishing Channels for Cooperation——Notes on the 2014 “Understanding & Cooperation” Dialogue

正From June 16 to 18,the 2014"UnderstandingCooperation"Dialogue and Forum on Rethinking Asia,co-hosted by CAFIU and FES and jointly organized with China Foundation for Peace and Development,was held in Tutzing,a beautiful and spacious manor located at 30km southwest of Munich.Nearly 60 delegates from more than 10Asian and European countries participated in the event,including China,Germany,Korea,Japan,