Identification of N,N-dimethylamphetamine formed by methylation of methamphetamine in formalin-fixed liver tissue by multistage mass spectrometry

Methamphetamine is methylated in the presence of unbuffered formalin solutions within hours at room temperature. The product, N,N-dimethylamphetamine, is also found in human liver exposed to methamphetamine followed by incubation with formalin. In the present study, a direct mass spectrometric method was developed to identify N,N-dimethylamphetamine in human liver before and after treatment with formalin. Human liver samples were obtained from four deaths that were investigated by the West Virginia Office of Chief Medical Examiner. Full toxicological analysis was conducted on samples from the decedents and methamphetamine was among the positive findings in each case. The method used to expose liver tissue to formaldehyde involved treating a small piece of liver from each case with formalin solution (20% v/v) for 24 h at room temperature. The formalin treated tissues were homogenized and the resulting suspension was sonicated for 5 min, and then centrifuged. Supernatant aliquots were directly analyzed by electrospray ionization (ESI) mass spectrometry without chromatographic isolation. Positive ion multistage mass spectra recorded in MS, MS/MS and MS/MS/MS (MS3) modes were used to confirm the presence of N,N-dimethylamphetamine and methamphetamine in the mixture. Liver tissue not treated with formalin did not contain a detectable level of N,N-dimethylamphetamine. Decreases in methamphetamine concentrations in liver tissue resulting from treatment with formalin were measured using deuterium-labeled methamphetamine as internal standard. The method can be completed in less than 2 h on thawed tissue. The results suggest that the process of fixing tissues with formalin may lead to false negative findings for methamphetamine.     

Participatory numbers for integrating knowledge and actions in development

This article discusses the opportunities and challenges associated with the use of quantitative participatory methods and participatory numbers for integrating locals’ and outsiders’ knowledge, as well as actions from the bottom-up and top-down, in development. It places emphasis on the contribution of quantitative participatory methods and participatory numbers to foster dialogue between a wide range of stakeholders, for example, local people, NGOs, government agencies and scientists, who usually seldom directly engage with each other. The article draws on case studies that discuss remittances and disasters in Samoa, and disaster risk reduction in the Philippines.     

Terrorism and Terrorist Leaders: Insights from Developmental and Ecological Psychology

A recent increase in terrorist actions where the terrorist's death is planned and intentional has raised interest in the psychological functioning, motivation, and reasoning of those who engage in terrorism and those who support it. No consensus exists among Western ¹ 1. That is, American or European. psychologists regarding terrorists who plan to die in their attack, and no Western psychological perspective has thus far contributed substantively to explaining or predicting it. Although most agree that groups engaging in such actions typically have a wider network of support, the psychology of supporters is also unexplained. This article proposes a developmental psychological model of the conditions that favor focused terrorist actions that have publicly discernible goals ascribed to a political or religious cause. The article describes a common pattern of cognitive complexity among terrorist leaders, using Osama bin Laden as a model, where entrenched cognitive simplicity in one key ideological domain (religious or political) is coupled with behavior reflecting the capacity for far greater complexity in other domains (organizational skills, planning, problem-solving.) This pattern, in specifiable historical and ecological context, makes terrorist tactics, including those in which the attacker intentionally dies, more likely, and increases the challenges associated with attempts at diplomacy or negotiated peace.