An ELISA method for the identification of salivary amylase

An ELISA method for the detection of salivary amylase in dried stains using a monoclonal anti-human salivary amylase antibody was developed. Studies demonstrated the assay to be sensitive down to 0.0002 Sigma units and showed a linear response between absorbance and salivary amylase activity between 0.002 and 0.2 units. The assay showed no cross reactivity with either commercially purchased human pancreatic or bacterial amylase. Sample studies utilizing swabs from several human body fluids showed that 100% of all saliva containing swabs (sixteen of sixteen) and 13% of non-saliva human body fluid swabs (eight of sixty-three) showed a net absorbance with the method. Of these eight non-saliva swabs yielding a net absorbance, none exceeded a salivary amylase activity of 0.003 units. In contrast, only three of the sixteen saliva-containing swabs (swabs produced from saliva diluted 1:5, 1:6, and 1:10, respectively) showed an activity below 0.2 units. Of these swabs, the 1:100 dilution showed the lowest activity (0.048). This value is still more than ten times that of the non-saliva containing swab with the highest activity.     

Prospects for Low-Income Mothers' Economic Survival Under Welfare Reform

This article discusses implications of data on the income andemployment patterns of welfare recipients for the types of programmaticand financial investments that states will need to make forsuccessful welfare reform. Research by the Institute for Women'sPolicy Research found that even before welfare reform, womenworked significant amounts of time and relied heavily on familysupports to survive, when possible. High school education andjob training are important predictors of having welfare andescaping poverty, while work experience alone has relativelylittle effect on leaving welfare. States will be challengedto provide these educational services within the restrictionson job training and education under the new welfare laws. Workingwelfare recipients in the institute's sample spent more thanone third of their income on child care, which speaks to theimportance of increased child-care subsidies for helping womenescape poverty. It is important for states and communities tomonitor the implementation of supportive services, track outcomesfor women who leave welfare, and improve work environments andemployment benefits.     

The government behind insurance governance: Lessons for ransomware

The insurance as governance literature focuses on the ability of private enterprises to collectively regulate, pool, and distribute risks. This paper analyzes how governments support insurance markets to maintain insurability and limit risks to society. We propose a new conceptual framework grouping government interventions into three dimensions: regulation of risky activity, public investment in risk reduction, and co-insurance. We apply this framework to six case studies, describing insurance markets' reliance on public support in more analytically precise terms. We analyze how mature insurance markets overcame insurability challenges akin to those currently presented by extortive cybercrime. Private governance struggled when markets grew too big for informal coordination or when (tail) risks escalated. Government interventions vary widely. Some governments prioritize supporting economic activity while others concentrate on containing risks. Governments also choose between risk reduction and ex post socialization of losses. We apply these insights to the market for ransomware insurance, discussing the merits and potential hazards of current proposals for government intervention.