Y-chromosome STR system, Y-PLEX 12, for forensic casework: development and validation

The Y-PLEX 12 system, developed for use in human identification, enables simultaneous amplification of eleven polymorphic short tandem repeat (STR) loci, namely DYS392, DYS390, DYS385 a/b, DYS393, DYS389I, DYS391, DYS389II, DYS 19, DYS439 and DYS438, residing on the Y chromosome and Amelogenin. Amelogenin provides results for gender identification and serves as internal control for PCR. The validation studies were performed according to the DNA Advisory Board's (DAB) Quality Assurance Standards. The minimal sensitivity of the Y-PLEX 12 system was 0.1 ng of male DNA. The mean stutter values ranged between 3.76-15.72%. A full male profile was observed in mixture samples containing 0.5 ng of male DNA and up to 400 ng of female DNA. Amelogenin did not adversely affect the amplification of Y-STRs in mixture samples containing male and female DNA. The primers for the Y-STR loci present in Y-PLEX 12 are specific for human DNA and some higher primates. None of the primate samples tested provided a complete profile at all 11 Y-STR loci amplified with the Y-PLEX 12 system. Y-PLEX 12 is a sensitive, valid, reliable, and robust multiplex system for forensic analysis, and it can be used in human forensic and male lineage identification cases.     

STR DNA typing: increased sensitivity and efficient sample consumption using reduced PCR reaction volumes

Improvements in detection limits/sensitivity and lower sample consumption are potential benefits of reducing PCR reaction volumes used in forensic DNA typing of crime scene samples. This premise was studied first with experimental mixtures and a nine-loci megaplex, which demonstrated stochiometric amplification and accurate detection. Next, adjudicated casework samples were subjected to amplification under 15 different template DNA to PCR reaction volume ratios. Reduction of PCR reaction volume and DNA down to 10 microL and 0.500 ng, respectively, produced identical profiles with the same signal intensity and heterozygous allele peak height ratio (HR). Reduction to 5 microL and 0.063 ng yielded HR values that were slightly affected in one to three STR loci. PCR reaction volume reduction can enhance detection and sensitivity while reducing the consumption of irreplaceable crime scene samples.     

Detecting Deception in Children's Testimony: Factfinders' Abilities to Reach the Truth in Open Court and Closed-Circuit Trials

This study examined the influence of closed-circuit television (CCTV) on jurors' abilities to detect deception in children's testimony. Children ages 7–9 individually played games and made a video movie with a male confederate. In the guilty condition, stickers were placed on exposed body parts (i.e., the child's arm, toes, and bellybutton). In the not-guilty and deception conditions, stickers were placed on the child's clothing rather than on bare skin. Approximately 3 weeks later, mock jurors recruited from the community viewed child participants testify either in a traditional courtroom setting or via one-way CCTV. The mock jurors responded to questions about the child witness and the defendant as well as deliberated to reach a verdict. Children in the deception condition were asked to testify as if the stickers had been placed on exposed body parts rather than on their clothing. Predeliberation, jurors were less likely to convict when a child testified in the deception condition as opposed to the guilty condition. These differences disappeared following deliberation. There was no support for the notion that jurors reach the truth better when children testify in open court versus via CCTV. Implications for jurors' abilities to reach the truth are discussed.     

Use of the MMPI-2 and the Inwald personality inventory to identify the personality characteristics of dropouts from a state police academy

The MMPI-2 and the Inwald Personality Inventory were employed to investigate the personality characteristics of dropouts from a state police academy. A traditional model of training borrowed from military models was used at the academy rather than a police generated model. Sensitive and independent individuals, more compatible with modern community policing methods may have rejected police work as a result of the experience. 15 academy completers and 9 dropouts were used in the sample. Analyses of the scales of the MMPI-2 and the Inwald Personality Inventory identified variables upon which the two groups differed. The hypothesis that more sensitive, empathic and independent individuals were leaving the academy appeared to be supported.     

Ideology and personality: Aspects of identity formation in adolescents with strong attitudes toward sex-role equalitarianism

Middle-class adolescent boys and girls with strong attitudes for and against the sex-role ideology of the Women's Movement were administered a Q-sort to study two aspects believed to be related to identity formation: flexibility-rigidity and independence-dependence. A significant positive relationship was found, more strongly for independence than for flexibility and more strongly for girls than for boys. More favorable attitudes toward sex-role equalitarianism were associated with flexibility and independence. The strength of the associations varied with the nature of the Q-sort: ideal self, self as ideal member of opposite sex, and self as ideal to each parent.This article is based on the dissertation by Dr. Kirsch in partial fulfillment of the Doctor of Philosophy Degree at the Graduate School of Education, University of Marylandm, College Park, MarylandPsychotherapist in private practice in Potomac, Maryland, with special interest in psychology of women. Received her Ph.D. in human development from the University of Maryland, Graduate School of Education, in 1974.Received his Ph.D. in clinical psychology from Boston University in 1955. Diplomate in clinical psychology (ABPP). Interest is adolescent development with emphasis on innovative programs for optimal growth as well as treatment services.Received his Ed.D. in human development from the University of Maryland in 1961. Current interest is in developing preservice education programs for Middle School personnel.     

Effect of similarity of ego identity status on interpersonal attraction

Similarity of ego identity status, assessed by Marcia's four-category classification system, was related to interpersonal attraction. Using male and female college students as subjects, this study found that (1) while all judges preferred targets who had or who are undergoing a crisis to those who have not had a crisis, (2) diffuse judges preferred targets with no commitments to those with commitments, and (3) judges with commitments preferred a foreclosure target more than judges without commitments. Differential evaluations of the targets' intelligence, knowledge of current events, adjustment, and morality were also found. Results are discussed both in terms of previous research positively relating personality similarity to attraction and Erikson's theory of the relationship between ego identity development and intimacy in interpersonal relations.     

Parental influences on the development of adolescent autonomy and identity

Two studies were undertaken to examine parental influences on autonomy and identity development. In Study 1, 262 adolescents in seventh and eleventh grades were given Kurtines's autonomy measure, Simmons's identity measure, and Elder's questions regarding the adolescents' perceptions of their parents' autocratic, democratic, or permissive parenting styles. Study 2 was a replication with 168 subjects. Across both studies it was found that sex-role socialization is more influential for automony development than is either level of parental power or age. Both age and father's use of democracy were the most influential variables on identity development.Received Ph.D. from the University of Minnesota. Major interests are adolescent social cognition and social development.Major interests are adolescent social cognition and social development.Major interests are adolescent social cognition and social development.Received Ph.D. from the University of Cincinnati. Major interests are cognitive development and theories of personality.     

Australian Aboriginal Property Rights as Issues of Indigenous Sovereignty and Citizenship

Aboriginal Australians have traditionally enjoyed little protection from the law. The matter of land has been at the heart of white settler/Aboriginal relations since the nation was first founded. It is only recently that recognition has been given to the land rights of Australian indigenous people. This recognition was finally made at the property law level in 1992 through the High Court decision in Mabo v. Queensland (n. 2) ([1992] 175 CLR 1). The 1993 High Court decision in The Wik Peoples v. Queensland ([1996] 71 ALJR 173) reinforced that recognition. It did so through the principle that pastoral lessees' and native title holders' rights might co-exist except that, in the event of any inconsistency, the pastoralists' rights were to prevail, provided pastoral activity was being pursued. The most recent legal change is the parliamentary revision of the Native Title Act so that the Wik co-existence principle was put to rest, mainly through permitting the State governments to upgrade pastoral holdings to a form of freehold, thus immunising them from native title claims, and minimising the payment of compensation. In this paper we argue that the country must consider what has been lost in this about-turn from the recognition of native title to land in Mabo . We argue that the nation must consider the emphases in the Mabo judgments upon the significance of international law and the need for the common law not to be locked into a racist past. From that point, we contend for the need to recognise not only native title to land but what lies beyond that: indigenous political and human rights.