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131.
A collaborative exercise was carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG) in order to evaluate the performance of two Y-chromosome STR PCR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The participating laboratories were asked to type three samples for the eight markers, using a specific amplification protocol. In addition, two control samples, with known haplotypes, were provided. The results obtained by the 13 different participating laboratories were identical, except for two laboratories that failed to type correctly the same two samples for GATA C4. By sequence analyses, two different GATA C4 allele structures were found. One control sample (allele 21) and two questioned samples (allele 22, correctly typed by all the laboratories, and allele 25) presented the following repeat structure: (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)n, but different from the one found for allele 26 in one sample included in this exercise, as well as in the second control sample (allele 23), namely (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)2(TGTA)2(TCTA)n. The collaborative exercise results proved that both Y-tetraplexes produce good amplification results, with the advantage of being efficiently typed using different separation and detection methodologies. However, since GATA C4 repeat presents a complex structure, with alleles differing in sequence structure, efficient denaturing conditions should be followed in order to avoid typing errors due to sizing problems.  相似文献   
132.
Alleles and haplotypes frequencies for 10 Y-chromosome STR loci (DYS19, DYS385 I/II, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS438 and DYS439), included in the Y-Plex6 and Y-Plex5 kits were determined for a Tunisian population sample of 100 male individuals.  相似文献   
133.
This paper contrasts the recent experiences of two different institutions in providing credit and savings facilities in the countryside of Tamil Nadu, southern India. Ever since the late-1960s, there has been a rising demand for credit to finance investment in agriculture. This has been reinforced by Government policy over the last two decades, and especially since 1980, to provide credit both to priority activities, including agriculture, and to disadvantaged rural groups. Part of that policy has been to encourage the commercial banks to offer services in the countryside, alongside the previously well-developed network of cooperatives offering formal rural credit. The commercial banks have had considerable success in expanding their network of branches, and in increasing bank deposits and loans in rural areas. Operating as corporate bureaucracies, the banks have been able to expand without being crippled in the process. On the other hand, their institutional strength has meant that Government credit policy has been implemented cautiously. The agricultural credit cooperatives, no newcomers to the countryside, have still to fulfil their long-declared function of providing short-term credit for crop production to the majority of agricultural households. Loan recovery by the cooperatives has been weak. The cooperatives have suffered partly from the internal contradictions inherent in any cooperative structure imposed upon the peasantry, from major flaws in the organizational structure of Tamil Nadu cooperatives, as well as from the increasing appropiation of the cooperatives for party political ends. The contrasting experiences of the two different institutions in providing rural credit illustrate how policy applied through different institutions can produce quite different outcomes. They also demonstrate how changes in the socio-political environment, neither immediately obvious nor predictable, can critically affect policy results.  相似文献   
134.
This article argues that for a number of reasons, the Russian state has had limited success in tackling the growing problem of HIV/AIDS. It begins with brief discussions of the health context of HIV/AIDS in the Russian Federation and the development of institutions in Russia post‐1991. It then discusses the attempts that have been made thus far to tackle the HIV/AIDS epidemic, both on the part of the federal government and with the aid of international donor funding. It is argued that a number of factors have contributed to Russia's lack of success in tackling HIV/AIDS. Firstly, the infection has been interpreted as a health problem rather than as a phenomenon which impacts on all aspects of public life. Secondly, there have been weak incentives at the federal level, which has resulted in a lack of a coordinated approach to HIV/AIDS at the national level. Thirdly, there have been regional variations in the autonomy and independence from the federal state impacting on the struggle against HIV in the Russian regions. Evidence from research undertaken by DFID is drawn upon to illustrate these variations in tackling HIV/AIDS. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   
135.
136.
Youth workers routinely experience moments in their professional practice with young people when, despite their experience and training, they are simply at a loss for what to do, how to respond, and how to be helpful to the youth. These experiences of not-knowing are seldom shared with other youth workers, which contributes to a climate of shame and humiliation. Professional supervision seldom attends to how youth workers experience these moments and their personal and vocational costs. This study presents a phenomenological investigation of how youth workers experience moments of not knowing what to do, identifies five central themes of the experience, and makes recommendations for improved youth work practice and supervision. This chapter presents the research question and its significance to the field of American youth work.  相似文献   
137.
This chapter provides a context for the concept of not-knowing, including a discussion of how the concept was framed. The experience of not-knowing in professional youth work is framed in relationship to other concepts explored by the social work and therapeutic literature (including vicarious trauma, helplessness, secondary trauma, and burnout), as well as those offered by the limited youth work and nursing literature discussing similar concepts (disruption and hurt, suffering, commitment in spite of conflict, and the struggle to go along when you do not believe). The standing of youth work in the professions and its own struggles to professionalize are explored, with attention to how not-knowing affects and is affected by these efforts.  相似文献   
138.
SUMMARY

Among youth workers who experience moments of not-knowing what to do, many often describe their thoughts and reactions to the phenomenon in vocational and existential terms. They ask what right they have to work in the helping professions if they find themselves simply unable to be helpful. In many cases, the vocational crises following experiences of not-knowing contribute to burnout and youth workers' decisions to leave the field altogether. This chapter describes the fourth of five themes associated with youth workers' experiences of not knowing what to do: questions of vocation. In addition to presenting the dominant theme, this chapter discusses the three variations on it, as described by youth worker participants: (a) What am I supposed to do? (b) Who am I to deal with this? and (c) Maybe the problem is me.  相似文献   
139.
140.
The dispossession of agricultural producers from the land has long been considered a condition of successful capitalist development. The main contention of this paper is that such dispossession has in fact become the source of major developmental handicaps for at least some and possibly many countries of the global South. We develop our argument by focusing on the South(ern) African experience as a paradigmatic outlier case of accumulation by dispossession—that is, as one of its extreme instances capable of highlighting in almost ideo-typical fashion its nature and limits. After reconstructing interpretations of capitalist development in Southern Africa that in the early 1970s established the region as a paradigm of accumulation by dispossession, we discuss how useful these interpretations are for understanding the more recent developmental trajectory of South Africa. We then suggest ways in which these interpretations from the 1970s should be reformulated in light of subsequent developments. We conclude by briefly examining the theoretical and policy implications of the analysis.  相似文献   
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