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811.
Six studies examined why and when respect vs. disrespect influences people’s emotions, self-worth, and behavior. Following relational models of justice, we argued that people use groups to derive information about the social self and as such value respect information because it indicates (a) whether or not they are accepted, and (b) how their status within the group is evaluated. These two identity concerns were operationalized by means of reinforcing people’s desire to belong (i.e., the identity concern of acceptance) and concern for reputation (i.e., the identity concern of one’s status evaluation). In line with predictions, the first three studies demonstrated that respect matters only among those whose concerns to belong are made salient. Studies 4–6 further showed that respect only influenced reactions among those who have strong concerns for reputation. It is concluded that respect communicates information relevant to people’s identity concerns—i.e., inclusion and reputation. 相似文献
812.
Krenke BE Viculis L Richard ML Prinz M Milne SC Ladd C Gross AM Gornall T Frappier JR Eisenberg AJ Barna C Aranda XG Adamowicz MS Budowle B 《Forensic science international》2005,148(1):1-14
Y chromosome-specific short tandem repeat (Y-STR) analysis has become another widely accepted tool for human identification. The PowerPlex Y System is a fluorescent multiplex that includes the 12 loci: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. This panel of markers incorporates the 9-locus European minimal haplotype (EMH) loci recommended by the International Y-STR User Group and the 11-locus set recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Described here are inter-laboratory results from 17 developmental validation studies of the PowerPlex Y System and include the following results: (a) samples distributed between laboratories and commercial standards produced expected and reproducible haplotypes; (b) use of common amplification and detection instruments were successfully demonstrated; (c) full profiles were obtained with standard 30 and 32 cycle amplification protocols and cycle number (24-28 cycles) could be modified to match different substrates (such as direct amplification of FTA paper); (d) complete profiles were observed with reaction volumes from 6.25 to 50 microL; (e) minimal impact was observed with variation of enzyme concentration; (f) full haplotypes were observed with 0.5-2x primer concentrations; however, relative yield between loci varied with concentration; (g) reduction of magnesium to 1mM (1.5 mM standard) resulted in minimal amplification, while only partial loss of yield was observed with 1.25 mM magnesium; (h) decreasing the annealing temperature by 2-4 degrees C did not generate artifacts or locus dropout and most laboratories observed full amplification with the annealing temperature increased by 2 degrees C and significant locus dropout with a 4 degrees C increase in annealing temperature; (i) amplification of individual loci with primers used in the multiplex produced the same alleles as observed with the multiplex amplification; (j) all laboratories observed full amplification with >or = 125 pg of male template with partial and/or complete profiles observed using 30-62.5 pg of DNA; (k) analysis of < or = 500 ng of female DNA did not yield amplification products; (l) the minor male component of a male/female mixture was observed with < or =1200-fold excess female DNA with the majority of alleles still observed with 10,000-fold excess female; (m) male/male mixtures produced full profiles from the minor contributor with 10-20-fold excess of the major contributor; (n) average stutter for each locus; (o) precision of sizing were determined; (p) human-specificity studies displayed amplification products only with some primate samples; and (q) reanalysis of 102 non-probative casework samples from 65 cases produced results consistent with original findings and in some instances additional identification of a minor male contributor to a male/female mixture was obtained. In general, the PowerPlex Y System was shown to have the sensitivity, specificity and reliability required for forensic DNA analysis. 相似文献
813.
Validation of male-specific, 12-locus fluorescent short tandem repeat (STR) multiplex 总被引:1,自引:0,他引:1
Krenke BE Viculis L Richard ML Prinz M Milne SC Ladd C Gross AM Gornall T Frappier JR Eisenberg AJ Barna C Aranda XG Adamowicz MS Budowle B 《Forensic science international》2005,151(1):111-124
Y chromosome-specific short tandem repeat (Y-STR) analysis has become another widely accepted tool for human identification. The PowerPlex Y System is a fluorescent multiplex that includes the 12 loci: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. This panel of markers incorporates the 9-locus European minimal haplotype (EMH) loci recommended by the International Y-STR User Group and the 11-locus set recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Described here are inter-laboratory results from 17 developmental validation studies of the PowerPlex Y System and include the following results: (a) samples distributed between laboratories and commercial standards produced expected and reproducible haplotypes; (b) use of common amplification and detection instruments were successfully demonstrated; (c) full profiles were obtained with standard 30 and 32 cycle amplification protocols and cycle number (24-28 cycles) could be modified to match different substrates (such as direct amplification of FTA paper); (d) complete profiles were observed with reaction volumes from 6.25 to 50 microL; (e) minimal impact was observed with variation of enzyme concentration; (f) full haplotypes were observed with 0.5-2x primer concentrations; however, relative yield between loci varied with concentration; (g) reduction of magnesium to 1mM (1.5 mM standard) resulted in minimal amplification, while only partial loss of yield was observed with 1.25 mM magnesium; (h) decreasing the annealing temperature by 2-4 degrees C did not generate artifacts or locus dropout and most laboratories observed full amplification with the annealing temperature increased by 2 degrees C and significant locus dropout with a 4 degrees C increase in annealing temperature; (i) amplification of individual loci with primers used in the multiplex produced the same alleles as observed with the multiplex amplification; (j) all laboratories observed full amplification with >or = 125 pg of male template with partial and/or complete profiles observed using 30-62.5 pg of DNA; (k) analysis of < or = 500 ng of female DNA did not yield amplification products; (l) the minor male component of a male/female mixture was observed with < or =1200-fold excess female DNA with the majority of alleles still observed with 10,000-fold excess female; (m) male/male mixtures produced full profiles from the minor contributor with 10-20-fold excess of the major contributor; (n) average stutter for each locus; (o) precision of sizing were determined; (p) human-specificity studies displayed amplification products only with some primate samples; and (q) reanalysis of 102 non-probative casework samples from 65 cases produced results consistent with original findings and in some instances additional identification of a minor male contributor to a male/female mixture was obtained. In general, the PowerPlex Y System was shown to have the sensitivity, specificity and reliability required for forensic DNA analysis. 相似文献
814.
Axel?MichaelowaEmail author Kristian?Tangen Henrik?Hasselknippe 《International Environmental Agreements: Politics, Law and Economics》2005,5(1):5-24
This article provides a background for the Developing post-2012 scenarios project, an international study which looks at a range of scenarios that countries may wish to consider for a post-2012 framework to tackle climate change. The purpose of the article is threefold. Firstly, it provides a review of the literature for the future climate regime. Secondly, it provides a set of criteria that are used in order to evaluate whether the scenarios provided in the other articles in this Special Edition are likely to be effective. Thirdly, the article spells out some of the more general policy implications rising from these scenarios. 相似文献
815.
Nele?MatzEmail author 《International Environmental Agreements: Politics, Law and Economics》2005,5(3):265-302
Environmental financing is one of the crucial issues of international environmental law and its implementation. From an environmental
perspective a prerequisite for success is that financial resources are used in an environmentally effective way. Whether the
World Bank, the Global Environment Facility (GEF) and the Prototype Carbon Fund (PCF) can be perceived as effective environmental
actors has to be measured by their objectives, their potential to promote accepted environmental standards and their relevant
funding practices. After significant improvements of their environmental policies, the World Bank must be considered the most
important institution for environmental financing due to its involvement in environmental trust funds but also in regard to
its regular lending practices. The GEF remains exceptional due to its institutional structure and scope, whereas the PCF is
an example of public–private partnerships that might be a model for future financing via trust funds. Since acceptance of
institutions can only be created if they are considered to be legitimate, legitimacy is closely tied to effectiveness. The
main criteria for legitimacy are state consent and the equality of states as well as supplementary considerations such as
transparency and public participation. From this perspective the World Bank, GEF and PCF structures of voting and participation
have come a long way, and despite their particularities and deficiencies they reflect to a varying degree elements of legitimate
decision making. 相似文献
816.
817.
Frequency of laryngeal and hyoid bone trauma in nonhomicidal cases who died after a fall from a height 总被引:1,自引:0,他引:1
de la Grandmaison GL Krimi S Durigon M 《The American journal of forensic medicine and pathology》2006,27(1):85-86
A retrospective study was carried out on 93 autopsy cases who died after a fall from a height. For each case, following parameters were reported: age, sex, manner of death, estimated height of fall, ground type, estimated site of primary impact, type of laryngeal and hyoid bone trauma, presence of associated local trauma in the neck and cephalic region. Mean age of the studied population was about 33 years. Sex ratio (men/women) was 1.8. Mean height of fall was about 15 m. Manner of death was, respectively, suicide and accident in 70 and 23 cases. The type of ground was concrete in 94% of the cases. The site of primary impact was, respectively, head, front of the body, back of the body, feet/lower limbs, and lateral body in 31, 26, 19, 12, and 5 cases. Cervical soft tissue bruising without laryngohyoid fractures was found in 5 cases. Laryngohyoid fractures were found in 5 cases. These fractures could be explained by direct impact of the neck structures against the ground. The mechanism of these fractures could also be indirect fractures due to high muscle strains on the hyoid bone or thyroid cartilage due to cervical hyperflexion or hyperextension or secondary to mandible or cervical vertebral column fractures. 相似文献
818.
- This article was initially a reaction to the special issues of the Journal of Public Affairs on the relative lack of theoretical foundations for the research and practice of public affairs (Vol. 1 no. 4, Vol. 2 no. 1, 2002).
- The social‐scientific approach that is known under the name constructionism has interesting implications for the study and the practice of this field. The authors explore some of its central concepts and implications such as framing, priming, typification, claims making, symbolic power, background, sense making and narrative construction. They test some of these concepts for their applicability and usefulness for research, theory building and practice in the field of public affairs.
819.
820.
Following Boyes and Chandler (1992), it is investigated how adolescents’ epistemic understanding relates to the process of identity formation. In a cross-sectional as well as longitudinal study, identity status scores of diffusion, foreclosure, moratorium and identity achievement as assessed by the Extended Objective Measure of Ego Identity Status were analyzed as a function of adolescents’ epistemic stance, while simultaneously taking into account individual differences in identity processing styles. The cross-sectional sample was composed of 200 German adolescents from Grades 7, 9, 11, and 13 (mean age: 16.2 years, SD = 2.41). The longitudinal study consisted of 134 participants (mean age at retest: 16.5 years, SD = 1.7) and covered a time interval of 18 months. Findings confirmed the view that the development of epistemic cognition contributes to adolescent identity formation. Specifically, the development of a multiplistic epistemic stance predicted higher moratorium scores, whereas the more advanced evaluativist epistemic position contributed to identity achievement over time. These effects were not due to individual differences in identity processing style. 相似文献