An improved method for post-PCR purification for mtDNA sequence analysis

Mitochondrial DNA (mtDNA) analysis of forensic samples typically is performed when the quantity and quality of DNA are insufficient for nuclear DNA analysis or when maternal relatives may be the only reference source. Many of the steps required in the analytical process are both lengthy and labor intensive. Therefore, improvements in the process that reduce labor without compromising the quality of the data are desirable. The current procedure requires purification of the amplicons by centrifugal filtration after PCR and prior to cycle sequencing. Because this method requires several manipulations to perform, alternate cleanup procedures were investigated. These include the use of 1) Qiagen QlAquick PCR Purification columns, 2) Concert Rapid PCR Purification columns, and 3) ExoSAP-IT reagent. When the yield of purified amplicons, quality of the sequence profile, and ease of assay were evaluated, the use of ExoSAP-IT reagent for post-amplification purification was chosen to replace the filtration method.     

Evaluation of some oxygen, sulfur, and selenium substituted ninhydrin analogues, nitrophenylninhydrin and benzo[f]furoninhydrin

Six ninhydrin analogues containing oxygen, sulfur, and selenium substituents at the C-5 position, 5-(4-nitrophenyl)ninhydrin, and benzo[f]furoninhydrin were evaluated as fingerprint development reagents. The analogues all showed good fingerprint color development but were not superior to ninhydrin in this respect. The benzo[f]furoninhydrin complex was strongly luminescent at room temperature following zinc complexation, while the remaining analogues required cooling to -196 degrees C to produce optimum luminescence. The benzo[f]furo, nitrophenyl, and methyl selenide analogues showed the best potential as fingerprint reagents with the benzo[f]furo analogue comparing favorably with DFO.     

Advisory Commission on Intergovernmental Relations in 1996: The End of an Era

On 30 September 1996 the U.S. Advisory Commission on IntergovernmentalRelations (ACIR) closed its doors, ending thirty-seven yearsof advocacy for federalism and intergovernmental relations.A majority of members in the Congress felt that A CIR had becomeirrelevant to the issues facing them and agreed that littlewould be lost by terminating the commission. The Clinton administration,although supportive until near the end, withdrew its'supportout of displeasure with the commission's handling of the unfundedfederal mandates issue. The national associations representingstate and local governments were ambivalent. ACIR was no longerlooked to for solutions to the nation's intergovernmental relationsproblems. With the exit of ACIR, the federal government's lastresource for addressing broad intergovernmental issues—beyondthe confines of individual programs—is gone.     

Blind Alleys: Can We Define Sovereignty?

Two recent works on sovereignty have argued that the term is indefinable and that all we can do is to analyse the definitions of others. The argument rightly centres upon the problematic link between sovereignty and the state. However it does not follow that because the whole notion of state sovereignty is problematic, sovereignty itself cannot be defined. This article suggests that a way out of this blind alley is to detach the idea of sovereignty from the institution of the state, so that an historically sensitive definition of the term becomes possible.     

Moderating effects of adolescent social orientation on the relation between social support and self-esteem

The study assessed whether the impact of social support on self-esteem is moderated by the adolescent's orientation toward the source of aid. Questionnaires were administered to 84 Israeli adolescents regarding self-esteem, perceived level of support from parents and peers, and preference, or orientation, for support from these sources. Regression analyses indicated that the positive effect of social figures' support on self-esteem increased as a function of interest in receiving aid from the specific source. Correlational analyses also revealed that heightened orientation toward parents was associated with higher levels of perceived parental support, whereas heightened orientation toward peers was associated with higher levels of peer support and lower levels of parental aid. These findings were consistent with self-evaluation maintenance and social provision theories, which suggest that the individual has an active role in selectively seeking out and filtering external social influences.His doctorate in educational psychology is from UCLA. Current research interests include stress and coping across the life span, social sources of adolescent self-esteem, and intergroup relations in the junior high school.Her doctorate in clinical psychology is from the Hebrew University in Jerusalem. Her research interests include family relations, social and emotional development in children and adolescents, and developmental psychopathology.Her research interests focus on social support in the family.     

Culture,self, and“URI”: Anti-Americanism in contemporary South Korea

As a much debated phenomenon in contemporary South Korea, anti-Americanism has been seen primarily as a response to the perceived political and economic domination of the United States in Korean affairs. This article suggests that such a view, however, is incomplete without consideration of the cultural and psychological context in which contemporary discourses of cultural nationalism have arisen: specifically, an indigenous cultural psychology characterized by an emphasis onuri (“we”)—a collective sense of socially diffuse yet unified and homogenous selfhood. As one variety of contemporary national cultural discourse, anti-Americanism is a response to certain to certain unwelcome trends in cultural development that have already begun to undermine the collective sense of Korean selfhood, as reflected in part in Korean concern over the Western “cultural invasion,” and Korean critiques of American bias and arrogance in dealings with Korea. However, far from being a static concept concerned only with defensive protection of Korean identity,uri also reflects Korean concern for re-formulating national cultural identity in terms more accommodating to the outside. Ultimately, anti-Americanism needs to be seen in the context of a Korean cultural psychology, which posits the enduring value of a collectively defined selfhood as an alternative to the prevailing individualist representations of the West. Diane M. Hoffman is an anthropologist and independent scholar with research interests in contemporary Korean culture and Korean-American intercultural relations.     

On Correcting Biases in Self-Reports of Age at First Substance Use with Repeated Cross-Section Analysis

Household survey data on age at first use of alcohol, tobacco, marijuana,and hard drugs can be biased due to sample selection and inaccuraterecall. One potential concern is attrition, whereby individuals who getinvolved with substance use at an early age become increasingly less likelyto be surveyed in successive years. A comparison of data from the NationalHousehold Survey on Drug Abuse (NHSDA) with data from a longitudinal studysuggested that attrition might have caused substantially less bias thandid forward telescoping, the inflating of age at first useover time. The evidence of forward telescoping was particularly pronouncedwith respect to age at first use of alcohol. This paper presents a procedurefor correcting the distribution of age at first use for forward telescoping(but not attrition) by viewing a portion of the NHSDA data collected insuccessive years as constituting a cohort study. Results are presented fromapplying this procedure with NHSDA data collected from 1982 to 1995 forrespondents born 1968–1973. The findings suggest that preventionprograms need to be introduced at an earlier age than would be indicatedby uncorrected retrospective data. Other implications are alsohighlighted.