全文获取类型
收费全文 | 45篇 |
免费 | 3篇 |
专业分类
各国政治 | 5篇 |
工人农民 | 1篇 |
世界政治 | 1篇 |
外交国际关系 | 2篇 |
法律 | 34篇 |
政治理论 | 4篇 |
综合类 | 1篇 |
出版年
2022年 | 2篇 |
2020年 | 2篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2015年 | 1篇 |
2014年 | 1篇 |
2013年 | 12篇 |
2012年 | 1篇 |
2011年 | 2篇 |
2010年 | 1篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2004年 | 2篇 |
2003年 | 3篇 |
2002年 | 2篇 |
1999年 | 1篇 |
1997年 | 1篇 |
1994年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1979年 | 1篇 |
排序方式: 共有48条查询结果,搜索用时 15 毫秒
1.
2.
3.
A bioinformatic tool was developed to assist with the victim identification initiative that followed the Swissair Flight 111 disaster. Making use of short tandem repeat (STR) DNA typing data generated with AmpFlSTR Profiler Plus (PP) and AmpFlSTR COfiler(CO) kits, the software systematically compared each available STR genotype with every other genotype. The matching algorithm was based on the search for: (i) direct matches to genotypes derived from personal effects; and (ii) potential kinship associations between victims and next-of-kin, as measured by allele sharing at individual loci. The software greatly assisted parentage analysis by enabling kinship evaluation in situations where complete parentage trios were unavailable and, in some situations, with distantly related relatives. Exclusion of fortuitous kinship associations (FKA) was made possible through the recovery at the disaster site of at least one remains for every sought-after victim, and was incorporated into the kinship software. The data from the 13 combined STR loci produced 6 and 23 times fewer FKAs when compared with PP alone and AmpFlSTR Profiler (PR) alone, respectively. Identification leads or confirmations of identification were obtained for 218 victims for which DNA reference samples (personal effects and kin) had been submitted. Confirmation of an inferred kinship association was sought through frequency and likelihood calculations, as well as corroborative data from other identification modalities. The use of a simple, yet powerful, automated genotype comparison approach and the use of megaplexes with high power of discrimination (PD) values extended considerably the identification capabilities in the case of the Swissair disaster. The DNA typing identification modality proved to be a valuable component of the large arsenal of identification tools deployed in the aftermath of this disaster. 相似文献
4.
To assist the interpretation of STR DNA typing results from forensic casework samples containing mixtures, the range of heterozygous allele peak height and peak area ratios (HR) and stutter percentages (stutter %) for the loci comprised in the AmpFlSTR Profiler Plus (PP) kit were assessed on 468 database and 275 casework single source samples. Stutter % medians were similar for database and casework samples, ranging from 2% to 7%. The upper limit of the stutter value range was 16%, calculated as median +3 SD, although lower locus-specific values could be used. HR medians were 93 +/- 6.5% for database samples, 88 +/- 12% for casework samples. For casework samples, the maximum signal imbalance noted was 52%, calculated as median -3 SD. No significant difference was observed between peak height and peak area calculated values. This study shows the importance of selecting the proper reference database for the establishment of HR threshold values. 相似文献
5.
Leclair B Sgueglia JB Wojtowicz PC Juston AC Frégeau CJ Fourney RM 《Journal of forensic sciences》2003,48(5):1001-1013
Improvements in detection limits/sensitivity and lower sample consumption are potential benefits of reducing PCR reaction volumes used in forensic DNA typing of crime scene samples. This premise was studied first with experimental mixtures and a nine-loci megaplex, which demonstrated stochiometric amplification and accurate detection. Next, adjudicated casework samples were subjected to amplification under 15 different template DNA to PCR reaction volume ratios. Reduction of PCR reaction volume and DNA down to 10 microL and 0.500 ng, respectively, produced identical profiles with the same signal intensity and heterozygous allele peak height ratio (HR). Reduction to 5 microL and 0.063 ng yielded HR values that were slightly affected in one to three STR loci. PCR reaction volume reduction can enhance detection and sensitivity while reducing the consumption of irreplaceable crime scene samples. 相似文献
6.
Chantal Bourassa 《Journal of family violence》2007,22(8):691-701
This study analyzed the impact of the co-occurrence of parental and interparental violence on the behavior of adolescents.
Results reveal that the co-occurrence of interparental violence and child physical abuse has a significantly greater negative
impact on behavior than does exposure to interparental violence only. Moreover, participants, who are both abused and exposed
to interparental violence, exhibit internalized and externalized symptoms falling within the clinical range more frequently.
Exposure exclusively to interparental violence also has definite impact; for example, teens who are only exposed to interparental
violence exhibit internalized and externalized symptoms more frequently than do those who have been neither subject to physical
abuse from a parent nor exposed to interparental violence. Implications of the findings are discussed. 相似文献
7.
Of ‘Strong’ Leadership,Crisis Communication,and Pooper Scoopers: Change in the Queensland Public Service Under Newman 下载免费PDF全文
Deanna Chantal Cristina Grant‐Smith Linda Katurah Colley 《Australian Journal of Public Administration》2018,77(2):236-252
Governments have historically offered their workforce a public service bargain founded on stable pay and conditions and job security. However, while the Westminster system aims for public service employment to be protected from the whims of government, public servants are nonetheless affected by the political environment in which they operate and changes to this bargain can occur with a new government. This paper focuses on a Queensland public service change process that resulted in large‐scale forced redundancies, non‐renewal of temporary contracts, and legislative changes that nullified the provisions in job security, organisational change, and redundancy policies. Using communication as a lens through which to understand leadership, it examines how the government communicated with the public service about this organisational change, immediately before and after the 2012 election. In particular, through analysis of public and media commentary, speeches and Hansard records, it examines Premier Newman's change leadership and communication by contrasting pre‐election messages to the public service with post‐election messages about the public service and the justifications for change during this period of downsizing. This analysis is used to reflect on political leadership, communication, fair treatment, and trust in public service change. 相似文献
8.
An Accelerated Analytical Process for the Development of STR Profiles for Casework Samples 下载免费PDF全文
Significant efforts are being devoted to the development of methods enabling rapid generation of short tandem repeat (STR) profiles in order to reduce turnaround times for the delivery of human identification results from biological evidence. Some of the proposed solutions are still costly and low throughput. This study describes the optimization of an analytical process enabling the generation of complete STR profiles (single‐source or mixed profiles) for human identification in approximately 5 h. This accelerated process uses currently available reagents and standard laboratory equipment. It includes a 30‐min lysis step, a 27‐min DNA extraction using the Promega Maxwell®16 System, DNA quantification in <1 h using the Qiagen Investigator® Quantiplex HYres kit, fast amplification (<26 min) of the loci included in AmpF?STR® Identifiler®, and analysis of the profiles on the 3500‐series Genetic Analyzer. This combination of fast individual steps produces high‐quality profiling results and offers a cost‐effective alternative approach to rapid DNA analysis. 相似文献
9.
Alexa Villavicencio-Queijeiro Chantal Loyzance Zoraida García-Castillo Jiro Suzuri-Hernndez Alejandra Castillo-Alanís Patricia Lpez-Olvera Fernanda Lpez-Escobedo 《Journal of forensic sciences》2022,67(1):217-228
In a context of rising violence and long-lasting impunity, in 2008, Mexico's criminal justice system underwent a radical change from an inquisitorial model to an adversarial one, to make it more effective, transparent, and expeditious. The new system tasked judges with publicly determining the admissibility of forensic evidence, as well as assessing its technical quality and probative value—tasks for which they currently receive little to no training. With the aim of contributing to the consolidation of the adversarial model, a comparative framework—in the form of a checklist—of the analysis of fingerprints, DNA samples, and voice recordings was created. To do so, a review of the academic literature, published reports, and guidelines was performed. The collected data were synthesized and submitted to a panel of Mexican judges, who provided feedback on its adequacy. The framework focuses on the steps on which quality assurance of forensic evidence depends, organized in five discrete stages that span from the collection of samples at the scene of a crime to the presentation of evidence at trial, specifying the main technical criteria experts should state to allow a decision maker to examine its accuracy and reliability. Differences and commonalities among the three methods were identified, particularly in terms of how qualitative and quantitative analyses are performed in each. Besides its potential usefulness as an aid for judicial decision-making, the checklist could be a valuable resource for training programs aimed at judges, as well as quality assurance programs. 相似文献
10.
Chantal Stebbings 《The Journal of legal history》2013,34(1):25-35
This essay examines some of the contexts in which women appear especially in non-royal deeds from the kingdom of Scotland in the years between roughly 1150 and 1350. It is based on a survey of several thousand charters written on behalf of both men and women, native, Anglo-Norman and European. It considers how charter evidence may be used to illuminate the extent to which women both used and shaped the laws and customs that governed the conveyance of land in medieval Scotland, and examines changes in women's legal capacity over time. Recent studies on literacy in later medieval Europe have shown that women participated actively in the literate culture of the period and that they exerted a sometimes profound influence on written texts themselves, and this theme is explored at some length in the context of Scottish charter evidence. Also examined is the extent to which traditional Gaelic customs in respect of women's property rights shaped and influenced the early common law of Scotland. 相似文献