Effectiveness of Coupled Application of AmpFℓSTR Yfiler Kit and Reduced Size Y‐chromosomal Short Tandem Repeat Analysis for Archeological Human Bones

The AmpF?STR Yfiler PCR Amplification (Yfiler) kit continues to be improved for a better analytical efficiency in cases of highly degraded DNA. The authors endeavored to determine whether coupling of the Yfiler kit with supplemental multiplex amplification of some Y‐STR loci is a more efficient analytical mode for poorly preserved human femurs (n = 15) discovered at Korean archeological sites. To reveal locus profiles not easily obtained by Yfiler analysis, custom‐designed primers were adopted for the DYS390, DYS391, DYS392, DYS438, DYS439, and DYS635 loci. The success rate for 16 Y‐STR locus profiles obtained from the 15 femurs was improved from 18.33% (in the use of Yfiler kit only) to 49.17% (the coupled use of Yfiler and custom‐designed primers). In this study, the authors established that the custom‐designed primers offer a markedly improved success rate for obtainment of Y‐STR profiles from degraded aDNA not easily identified by sole use of the Yfiler assay.     

Application of mRNA Expression Analysis to Human Blood Identification in Degenerated Samples that were False‐negative by Immunochromatography,,

Forensic laboratories are often faced with cases in which methamphetamine hydrochloride‐mixed blood is unable to be identified as human blood by immunochromatography against human hemoglobin A0. The application of mRNA expression analysis to samples that showed a false‐negative with immunochromatography was investigated as an alternative approach that did not depend on the antigen–antibody reaction. Real‐time PCR was used to examine the expression levels of blood markers such as glycophorin A, spectrin beta, and hemoglobin beta. Hemoglobin beta was the only marker that was specifically detected in blood, while glycophorin A was useful for determining human specificity. Hemoglobin beta showed good detection sensitivity and was detectable in 37‐year‐old blood stains. Hemoglobin beta was exclusively detectable in methamphetamine hydrochloride‐mixed blood stains. Detergents and disinfectants did not significantly influence mRNA markers. The proposed mRNA expression analysis was suitable for human blood identification as an alternative method to immunochromatography.     

Multilateral CBMs on the Korean Peninsula: Making a virtue out of necessity

Abstract

As its economy has become near to collapse, North Korea has tried to avoid direct contacts with South Korea because of the ‘absorption phobia’. Instead, the North has made continuous efforts to improve its relations only with the United States, seeking a guarantee for its survival. Given this circumstance, this paper argues that useful multilateral approaches such as KEDO and Four‐Party Talks will contribute to improving inter‐Korean relations. Thus, it would be sensible to explore every possible way (even through multilateral mechanisms) until both Koreas make a breakthrough for the deadlocked inter‐Korean CBMs. But the multilateral CBMs constitute a transitional and complementary role as South and North Korea should be primarily responsible for addressing major problems such as reunification. Among the multilateral approaches, the Four‐Party Talks will be a most useful mechanism which will enable the two Koreas to resume dialogue for the peace and reunification on the Korean Peninsula. In this peace process, more positive roles of major powers are also requested.     

Socioeconomic development,infant mortality and fertility: A cross‐sectional and longitudinal analysis of 63 selected countries

Data from 63 selected countries of the world at two different points in time, 1958 and 1968, are used to test a path model in which infant mortality acts as an intermediate variable between two composite indicators of economic and social development and the fertility rate. The model is tested with both cross‐sectional and longitudinal data. In order to ascertain the appropriate time dimension in which the independent variables influence the trends of fertility, the results from an instantaneous model are compared with those from a lagged effect model.     

Population data of nine miniSTR loci in Koreans

For highly degraded DNA samples of forensic casework, new miniSTR systems have been developed to supplement the current STR systems. In the present study, nine miniSTR loci were analyzed in 300 unrelated Koreans using three multiplex PCR systems (multiplex I: D10S1248, D14S1434 and D22S1045; multiplex II: D1S1677, D2S441 and D4S2364; and multiplex III: D3S3053, D6S474 and D20S482), and allele frequencies and forensic parameters were calculated. These data demonstrated that D10S1248, D2S441, D22S1045, D14S1434, and D6S474 are as highly informative as the CODIS STRs suggesting that the miniSTRs could be useful for forensic analysis of degraded DNA.     

Legislative voting in the pork-dominant parliament: evidence from the Philippine House of Representatives

In this paper I investigate how legislators behave in extremely pork-oriented, or pork-dominant, systems where virtually no party-line voting takes place and politicians strive to deliver individual/local benefits instead of national public policies. I argue that, in the pork-dominant context, most legislators vote with the president, who controls access to the pork pipeline, irrespective of their party affiliations. Thus, the president’s party legislators should have little incentive to vote against the president, regardless of voter demands for pork or policy; however, sometimes opposition-party legislators elected particularly from more-developed districts (where voters often desire policy over pork) should have strong incentives to vote against the president. These arguments are supported with quantitative studies of the post-authoritarian Philippine House of Representatives. It is found that, in the highly homogeneous legislature dominated by pork-seeking politicians, (1) most bills are passed with unanimous or near-unanimous consent, (2) governing-party legislators almost always vote with the majority of the members of the parliament, regardless of voters’ socio-economic conditions, and (3) opposition-party legislators, especially in more-developed areas, tend to vote against the parliamentary majority. These findings indicate that voters’ high socio-economic status promotes programmatic accountability, leading their representatives to undermine voting unity within the extremely cohesive pork-dominant legislature.     

State,Society, and Economic Development in Sports Life Cycles: The Case of Boxing in Korea

This study documents the rise and fall of boxing in Korea since its introduction in 1912. The participation of amateur athletes in boxing has decreased sharply since the 1980s. Also, the popularity of professional boxing among sports fans has diminished in recent decades. I consider boxing as a “product” that is “consumed” by individuals as participants and fans. I apply product life-cycle theory in analyzing the changing popularity of boxing. I argue that changes in the tastes of sports fans are closely linked to economic development, industrialization and standard of living. I present the rise in the popularity of soccer, baseball, and golf as illustrations of the changing tastes of consumers of sports that have accompanied economic development and social changes as well as the changing government policies on sports.

Selection of twenty-four highly informative SNP markers for human identification and paternity analysis in Koreans

A number of DNA marker types suitable for human identification and parentage testing have been developed, of which single nucleotide polymorphisms (SNPs) merit attention as they are abundant, genetically stable, and amenable to high-throughput automated analysis. In this regard, 24 highly informative SNP markers representing each 22 autosome and both sex chromosomes were selected, and the allele and genotype frequencies of these SNPs were determined in a group composed of 30 unrelated Koreans. Based on frequency data from this group, the estimated probability of identity (P(I)) and probability of paternity exclusion (P(E)) with 22 autosomal SNP loci were 1.905x10(-10) and 98.9%, respectively. The SNPs in this study offer a small but highly accurate database that will be an essential reference for SNP-based forensic application in the future.