The determination of lysergide (LSD) in urine by high-performance liquid chromatography-isotope dilution mass spectrometry (IDMS)

The use of isotope dilution mass spectrometry (IDMS) has been investigated for the forensic confirmation of lysergic acid diethylamide (LSD) in urine by LC-MS. The advantages of using a deuterated analog of LSD as an internal standard over methysergide are discussed. This study includes a comparison of the electrospray mass spectra of LSD, LSD-d3 and methysergide, and discusses the choice of suitable ions for use in selected ion monitoring (SIM) mode. An IDMS method is presented for the LC-MS confirmation of LSD in urine, with a limit of quantification (LOQ) of 0.5 ng/mL, reflecting the forensic requirement at this laboratory. Under some circumstances the LOQ can be improved to 0.1 ng/mL. This method is linear in the range tested (up to 10 ng/mL LSD in urine) and has been validated in terms of accuracy and precision.     

A novel method for STR-based DNA profiling using microarrays

We describe a novel method for rapidly identifying and distinguishing between different DNA sequences using short tandem repeat (STR) analysis and DNA microarrays. The method can be used to deduce identity, length, and number of STRs of the target molecule. We refer to this technique as the "variable-length probe array" method for STR profiling (VLPA). The method involves hybridization of the unknown STR target sequence to a DNA microarray displaying complementary probes that vary in length to cover the range of possible STRs. A post-hybridization enzymatic digestion of the DNA hybrids is then used to selectively remove labeled single-stranded regions of DNA from the microarray surface. The number of repeats in the unknown target is then deduced based on the pattern of target DNA that remains hybridized to the array. This DNA profiling technique is useful for performing forensic analysis to uniquely identify individual humans or other species.     

Book notes

Political Participation and Democracy in Britain by Geraint Parry, George Moyser and Neil Day. Cambridge: Cambridge University Press, 1992. Pp.xviii + 510; index, bibliography, three appendices. £60 (hardback); £17.95 (paperback). ISBN 0 521 33298 2 and 33602 3

Work and Citizenship in the New Europe edited by Harry Coenen and Peter Leisink. Aldershot: Edward Elgar, 1993. Pp.254; index, bibliography. £38.50. ISBN 1 85278 739 2

Capitalist Development and Democracy by Dietrich Rueschemeyer, Evelyne Huber Stephens and John D. Stephens. Cambridge and Oxford: Polity Press, 1992. Pp.387; index, bibliography, appendices. £45 (hardback); £14.95 (paperback). ISBN 0 7456 0398 X

Minority Representation and the Quest for Voting Equality by Bernard Grofman, Lisa Handley and Richard G. Niemi. Cambridge, London and New York: Cambridge University Press, 1992. Pp.174; index, bibliography. £30. ISBN 0 521 39128 8

Peace, Politics and Violence in the New South Africa edited by Norman Etherington. London: Hans Zell Publishers, 1992. Pp.352; index. £45. ISBN 1 873836 75 6

The State in Africa: The Politics of the Belly by Jean‐François Bayart. London and New York: Longman, 1993. Pp.370; index. £14.99 (paperback). ISBN 0 582 06421 X

Russia in Flux: The Political and Social Consequences of Reform edited by David Lane. Aldershot: Gower, 1992. Pp.xvi + 248; index. £45 (hardback); £14.95 (paperback). ISBN 1 85278 680 9 and 713 9

Democracy and Development by Axel Hadenius. Cambridge: Cambridge University Press, 1992. Pp.xi + 218; index, bibliography, appendices. £30. ISBN 0 521 41685 X

Power and Empowerment: A Radical Theory of Participatory Democracy by Peter Bachrach and Aryeh Botwinick. Philadelphia, PA: Temple University Press, 1992. Pp.ix + 211; index, bibliography. ISBN 0 87722 930 9

Democratization in Africa: African Views, African Voices edited by Sahr John Kpundeh. Washington, DC: National Academy Press, 1992. Pp.85. £17.95 (paperback). ISBN 0 309 04797 8

Marxism and Democracy by Joseph V. Femia. Oxford: Clarendon Press, 1993. Pp.204; index, bibliography. £25 (hardback). ISBN 0 19 827494 7

Dirty Politics: Deception, Distraction and Democracy by Kathleen Hall Jamieson. New York and Oxford: Oxford University Press, 1992. Pp.335; index, bibliography, appendices. $25 (hardback). ISBN 0 19 597854 3

Viva ‐ Women and Popular Protest in Latin America edited by Sarah A. Radcliffe and Sallie Westwood (International Studies of Women and Place Series). London and New York: Routledge, 1993. Pp.270; index, bibliography. £40 (hardback); £12.99 (paperback). ISBN 0 415 07312 X

Dimensions of Radical Democracy edited by Chantal Mouffe. London: Verso, 1992. Pp.254; bibliography. £12.99 (paperback). ISBN 0 86091 556 5

Democracy and Discontent: India's Growing Crisis of Governability by Atul Kohli. Cambridge: Cambridge University Press, 1990. Pp.420; index, bibliography. £35 (hardback). ISBN 0 521 39161 X     

Note on the preservation of Persian Fauna

The Venture of Islam. Conscience and History in a World Civilisation. 3 volumes. By Marshall G. S. Hodgson. Chicago and London, Chicago University Press, 1975. Pp. 532,609 and 496. Maps. Index. £30.00 (3 vols.).