The use of DNA stabilizing solution to enable room temperature storage and transportation of buccal and trace sample swabs

It is proposed that a DNA stabilizing solution (DNA Genotek Inc.) designed to preserve DNA in saliva samples at room temperature can be extrapolated to the storage of swab heads. The aim of this study was to evaluate the effectiveness of the solution for the preservation of reference swabs (buccal) and trace samples (facial swabs). To this end, the solution was used during a twin-site DNA transfer project assessing background levels of carer DNA present in children. Tubes containing 400 μl of solution were used to store and transport swab heads. At the laboratory, samples were extracted using the QIAamp DNA Mini Kit (Qiagen), quantified using the Quantifiler Duo Kit and profiled using the AmpF?STR^® SGM Plus^® PCR Amplification Kit (both Applied Biosystems). Twenty-eight PCR cycles were applied to all samples. Thirty-four cycles or a longer electrophoresis injection time was applied to trace samples where necessary. All Reference swabs produced high quantities of DNA and full DNA profiles after 28 cycles. Profile morphology indicated good quality DNA with no degradation. Of the trace samples, sufficient profiles were achieved to study the transfer of carer DNA making the solution fit for continued use in this project. DNA stabilizing solution enables the storage and transportation of swabs without freezing. This is convenient, reduces transportation costs and enables instant analysis of samples upon arrival at the laboratory. This is a useful alternative for a multi-site research project as well as a reliable storage tool for use in remote areas.     

Computer‐aided Dental Identification: An Objective Method for Assessment of Radiographic Image Similarity*

Abstract: A pilot study evaluated a computer‐based method for comparing digital dental images, utilizing a registration algorithm to correct for variations in projection geometry between images prior to a subtraction analysis. A numerical assessment of similarity was generated for pairs of images. Using well‐controlled laboratory settings, the method was evaluated as to its ability to identify the correct specimen with positive results. A subsequent clinical study examined longitudinal radiographic examinations of selected anatomical areas on 47 patients, analyzing the computer‐based method in making the correct identification based upon a threshold level of similarity. The results showed that at a threshold of 0.855, there were two false negative and two false positive identifications out of 957 analyses. Based on these initial findings, 25 dental records having two sets of full mouth series of radiographs were selected. The radiographs were digitized and grouped into six anatomical regions. The more recent set of films served as postmortem images. Each postmortem image was analyzed against all other images within the region. Images were registered to correct for differences in projection geometry prior to analysis. An area of interest was selected to assess image similarity. Analysis of variance was used to determine that there was a significant difference between images from the same individual and those from different individuals. Results showed that the threshold level of concordance will vary with the anatomical region of the mouth examined. This method may provide the most objective and reliable method for postmortem dental identification using intra‐oral images.     

Administrative burden in digital public service delivery: The social infrastructure of library programs for e-inclusion

In their efforts to digitize public service delivery, countries increasingly use algorithms based on mathematical models, data and/or a combination of different administrative datasets to issue decisions, but recent studies point towards challenges around citizens' understanding, accessing, and filing objections to such automated decisions. This paper focuses on the social infrastructure supporting citizens that struggle with accessing such services. To address this, we ask: How does the social infrastructure affect administrative burdens associated with digital government services? This is studied in the Dutch context through expert interviews and observations of support programs in libraries. We find that although libraries as primary sites for these services may pose the disadvantage of being more difficult to reach for low-literate citizens, advantages are their organizational structure at the local level as well as their currently changing role to include a growing range of services, including (digital) skills courses.     

‘For good measure’: data gaps in a big data world

Policy and data scientists have paid ample attention to the amount of data being collected and the challenge for policymakers to use and utilize it. However, far less attention has been paid towards the quality and coverage of this data specifically pertaining to minority groups. The paper makes the argument that while there is seemingly more data to draw on for policymakers, the quality of the data in combination with potential known or unknown data gaps limits government’s ability to create inclusive policies. In this context, the paper defines primary, secondary, and unknown data gaps that cover scenarios of knowingly or unknowingly missing data and how that is potentially compensated through alternative measures. Based on the review of the literature from various fields and a variety of examples highlighted throughout the paper, we conclude that the big data movement combined with more sophisticated methods in recent years has opened up new opportunities for government to use existing data in different ways as well as fill data gaps through innovative techniques. Focusing specifically on the representativeness of such data, however, shows that data gaps affect the economic opportunities, social mobility, and democratic participation of marginalized groups. The big data movement in policy may thus create new forms of inequality that are harder to detect and whose impact is more difficult to predict.

     

Cognitive Abilities,Social Adaptation,and Externalizing Behavior Problems in Childhood and Adolescence: Specific Cascade Effects Across Development

Children’s and adolescents’ cognitive abilities, social adaptation, and externalizing behaviors are broadly associated with each other at the bivariate level; however, the direction, ordering, and uniqueness of these associations have yet to be identified. Developmental cascade models are particularly well-suited to (1) discern unique pathways among psychological domains and (2) model stability in and covariation among constructs, allowing for conservative tests of longitudinal associations. The current study aimed to identify specific cascade effects among children’s cognitive abilities, social adaptation, and externalizing behaviors, beginning in preschool and extending through adolescence. Children (46.2?% female) and mothers (N?=?351 families) provided data when children were 4, 10, and 14 years old. Cascade effects highlighted significant stability in these domains. Unique longitudinal associations were identified between (1) age-10 cognitive abilities and age-14 social adaptation, (2) age-4 social adaptation and age-10 externalizing behavior, and (3) age-10 externalizing behavior and age-14 social adaptation. These findings suggest that children’s social adaptation in preschool and externalizing behavior in middle childhood may be ideal intervention targets to enhance adolescent well-being.     

Reflections on “The Politics of Informality”: What We Know,How We Got There,and Where We Might Head Next

Building on the methodological and empirical contributions of the various authors in this special symposium, this concluding reflection acknowledges the important role that informality plays in urban and national politics in the global South, even as it proposes a range of alternative ways, this critical topic could and should be inserted into contemporary scholarship in comparative politics. It begins with a discussion of two decades of research on urbanization and economic globalization, thus introducing a wider set of disciplinary concerns than merely urban servicing into the study of informality, ranging from the transformation of property rights regimes in the context of ascendant neo-liberalization to the recent emergence of more decentralized political structures for claim-making and governance. The essay then suggests that greater historical and contextual specificity in the study of informality, along with the methodological innovations highlighted in the papers, will further help reveal the range of responses to informality seen across the different case studies. Specifically, it proposes that closer attention to divergent urban and national pathways of democratization, attention to institutional variations within and across democratic regimes, political party dynamics at the local and national level, and the existence of urban violence, among other factors, will help explain how and why bureaucrats and elected officials may choose to deal differently with the existence of informality. The essay concludes by arguing that informality should be considered as both a form of governance and a means of enacting citizenship. It thus asks scholars to question the longstanding conceptual dichotomies that permeate much of the literature on informality, including the stark conceptual divide between the formal and informal, and instead to recognize that complex, interactive, and iterative relationships between citizens and the state in the arena of informality are what drive urban servicing and sociopolitical change.     

Lesbian,Gay and Bisexual Populations: The Role of English Local Government

Abstract

The role of local government has recently come under debate, in the context of state retrenchment, public sector cuts and the marketisation of welfare. Recent discussions have centred on local authorities as leaders, on enterprise and on democracy, but there has been little discussion of local authorities in relation to equalities issues and minority groups. This article examines the role of local government in relation to one minority grouping, Lesbian, Gay and Bisexual (LGB) people, drawing on empirical material gathered from 2007 to 2010 as part of a large Economic and Social Research Council research project. It describes the importance of equalities legislation and related implementation mechanisms in driving forward the LGB equalities agenda, explores aspects of welfare delivery to LGB people and addresses democratic processes. The article suggests that a collision of different forces is currently taking place: the legislation supports the protection of the LGB communities, but this support is undermined by the recession-related and ideologically driven public sector cuts. In addition, aggregate approaches to local democracy may override the interests of minority groups such as LGB people.     

Beyond Correlates: A Review of Risk and Protective Factors for Adolescent Dating Violence Perpetration

Dating violence is a serious public health problem. In recent years, the U.S. Centers for Disease Control and Prevention and other entities have made funding available to community based agencies for dating violence prevention. Practitioners who are tasked with developing dating violence prevention strategies should pay particular attention to risk and protective factors for dating violence perpetration that have been established in longitudinal studies. This has been challenging to date because the scientific literature on the etiology of dating violence is somewhat limited, and because there have been no comprehensive reviews of the literature that clearly distinguish correlates of dating violence perpetration from risk or protective factors that have been established through longitudinal research. This is problematic because prevention programs may then target factors that are merely correlated with dating violence perpetration, and have no causal influence, which could potentially limit the effectiveness of the programs. In this article, we review the literature on risk and protective factors for adolescent dating violence perpetration and highlight those factors for which temporal precedence has been established by one or more studies. This review is intended as a guide for researchers and practitioners as they formulate prevention programs. We reviewed articles published between 2000 and 2010 that reported on adolescent dating violence perpetration using samples from the United States or Canada. In total, 53 risk factors and six protective factors were identified from 20 studies. Next steps for etiological research in adolescent dating violence are discussed, as well as future directions for prevention program developers.     

Irish Divorce and Domestic Violence, 1857–1922

Ireland was excluded from the Divorce and Matrimonial Causes Act of 1857, which moved divorce proceedings from parliament to court. Whilst other areas of the Empire were encouraged to follow the 1857 reform, Ireland retained the costlier, lengthier and more socially and gender biased parliamentary process. Louisa Westropp's divorce of 1886 was the first divorce granted by Westminster to an Irishwoman and established legal precedent in applying case law, initially concerning domestic violence, derived in the divorce court to parliament. The result was an increase in Irish parliamentary divorce petitions, particularly from women, who utilised an augmenting definition of marital cruelty to secure a permanent release from spousal abuse.