Detection and Identification of Psilocybe cubensis DNA Using a Real‐Time Polymerase Chain Reaction High Resolution Melt Assay,

Psilocybe cubensis, or “magic mushroom,” is the most common species of fungus with psychedelic characteristics. Two primer sets were designed to target Psilocybe DNA using web‐based software and NBCI gene sequences. DNA was extracted from eighteen samples, including twelve mushroom species, using the Qiagen DNeasy^® Plant Mini Kit. The DNA was amplified by the polymerase chain reaction (PCR) using the primers and a master mix containing either a SYBR^® Green I, Radiant? Green, or LCGreen Plus^® intercalating dye; amplicon size was determined using agarose gel electrophoresis. The PCR assays were tested for amplifiability, specificity, reproducibility, robustness, sensitivity, and multiplexing with primers that target marijuana. The observed high resolution melt (HRM) temperatures for primer sets 1 and 7 were 78.85 ± 0.31°C and 73.22 ± 0.61°C, respectively, using SYBR^® Green I dye and 81.67 ± 0.06°C and 76.04 ± 0.11°C, respectively, using Radiant? Green dye.     

Kinematics of Signature Writing in Healthy Aging

Forensic document examiners (FDE) called upon to distinguish a genuine from a forged signature of an elderly person are often required to consider the question of age‐related deterioration and whether the available exemplars reliably capture the natural effects of aging of the original writer. An understanding of the statistical relationship between advanced age and handwriting movements can reduce the uncertainty that may exist in an examiner's approach to questioned signatures formed by elderly writers. The primary purpose of this study was to systematically examine age‐related changes in signature kinematics in healthy writers. Forty‐two healthy subjects between the ages of 60–91 years participated in this study. Signatures were recorded using a digitizing tablet, and commercial software was used to examine the temporal and spatial stroke kinematics and pen pressure. Results indicated that vertical stroke duration and dysfluency increased with age, whereas vertical stroke amplitude and velocity decreased with age. Pen pressure decreased with age. We found that a linear model characterized the best‐fit relationship between advanced age and handwriting movement parameters for signature formation. Male writers exhibited stronger age effects than female writers, especially for pen pressure and stroke dysfluency. The present study contributes to an understanding of how advanced age alters signature formation in otherwise healthy adults.     

Three to Four Year Prospective Evaluation of Personality and Behavioral Risk Factors for Later Disordered Eating in Adolescent Girls and Boys

Findings of a 3- to 4-year prospective investigation of personality, temperament, and behavioral factors predictive of the later development of disordered eating in an adolescent population are presented. The sample consisted of 726 girls and 698 boys who entered the study in grades 7–10 in year 1 or in grade 7 in year 2. Predictors of eating disorder risk score were determined separately by gender. For both girls and boys, the latent variable of negative affect/attitudes determined at study entrance was the only significant predictor of final-year risk score. Semistructured diagnostic interviews confirmed an eating disorder diagnosis in 52.8% of 36 female subjects in the high eating disorder symptom group. A substantial history of lifetime and current comorbidity also was noted in this group. The function of negative affect/attitudes as a generalized psychopathology vulnerability factor and as a specific factor increasing risk for disordered eating is discussed.