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181.
Morphine-3-D glucuronide stability in postmortem specimens exposed to bacterial enzymatic hydrolysis
Carroll FT Marraccini JV Lewis S Wright W 《The American journal of forensic medicine and pathology》2000,21(4):323-329
Medical examiners frequently rely on the finding of free morphine present in postmortem specimens to assist in certifying deaths associated with narcotics. In vitro hydrolysis of morphine-3-D glucuronide (M3DG) to free morphine was studied using variable specimen pH, initial degree of specimen putrefaction, storage temperature and time, and the effectiveness of sodium fluoride (NaF) preservation. Reagent M3DG was added to opiate-free fresh blood and urine and to autopsy-derived blood specimens. Reagent bovine glucuronidase was also added to certain specimens. Freshly collected and refrigerated NaF-preserved blood produced minimal free morphine, whereas four of five autopsy blood specimens produced free morphine from M3DG. Increased storage time, temperature, and initial degree of putrefaction resulted in greater free morphine generation despite the absence of viable bacteria. Hydrolysis occurring during specimen storage can generate free morphine from M3DG and may result in erroneous conclusions in certifying narcotic deaths. 相似文献
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Trucking deregulation, in the interest of competition and efficiency,removes rate controls and grants free entry to the market. Whenthe United States deregulated trucking in 1980, it opened theinterstate market to Canadian carriers. Heavy volumes of tradeby trucking between the two countries make entry conditionsan important bilateral issue. Deregulatory symmetry betweenthe two countries would produce a de facto regime of free tradein trucking services. In 1987 the Canadian federal governmentadopted deregulatory measures similar to those of the UnitedStates, but with more complex and problematic results. The reasons,broadly characteristic of the evolution of Canadian federalism,lie in the ability of the provinces to thwart federal initiatives.Trucking regulation is under provincial control, and to achievea practical effect, the federal government depends upon theprovinces to bring their practices into accord with its policy.Because of differing provincial views about deregulation andpressures from Canadian truckers for continued protection fromAmerican competition, much diversity and contention have delayedand compromised the federal governments purpose. Regulatoryreciprocity is also a question of the provinces and states,with a mixture of strict and easy entry policies complicatingthe achievement of bilateral balance and equity. The largerimplications of deregulation and transborder trucking for Canadalie in the ability of a decentralized federal system to pursuenecessary initiatives coherently. 相似文献
184.
Abstract: The argument presented in this article is that the appointment of an ad hoc expert commission to carry out governance is unlikely to depoliticize difficult restructuring issues or to deflect blame from governments dealing with such problems in Westminster-style polities. Unlike in American-style presidential systems and parliamentary systems with proportional representation experiencing frequent minority governments, such commissions can never be truly independent as there are no serious checks on the government's ability to remake the agency, its mandate, its composition, nor even any barriers to the government's premature termination of an ad hoc expert commission's authority. When governments in a Westminster-style polity seek to establish the appearance that such a commission is an independent agency of governance they must work at cross-purposes to the basic rules for insuring accountability by giving such a body a very vague mandate. This will almost certainly lead to disputes between political actors and the commission over its powers and refocus blame on the government. The ministers of a government employing this strategy must also be extraordinarily careful so as not to engage in any activities that would undermine the ad hoc expert commission's already fragile claim to autonomy, otherwise the blame focused on the government will magnify even further. The difficulties involved in employing an ad hoc expert commission as a means to depoliticize decisions and as a blame-avoidance strategy for governments in Westminster-style polities are illustrated in the Ontario government's experience with the Health Services Restructuring Commission (hsrc). 相似文献
185.
The volunteer movement in criminal justice settings is burgeoning. It has been estimated that by the end of 1982 there will be over one million volunteers working in criminal-justice-related functions. The volunteer role that appears to be increasing most rapidly is that of the one-to-one counseling relationship with delinquent and young adult offenders on formal or informal probation. While the demand for volunteer probation officers is increasing rapidly, few of our courts have attempted to develop and validate screening instruments capable of assessing and predicting the general competency of the volunteer. The need for such instruments has been emphasized recently by Judge Keith J. Leenhouts, Director of Volunteers in Probation, a division of the National Council on Crime and Delinquency. The present study attempts to assess the usefulness of one such potential screening device, the Critical Incident Response Test (CIRT) developed by Traitel (1972) and others at the Oakland County Juvenile Court in Pontiac, Michigan. It is hoped that this study will motivate other probation departments and court volunteer programs to develop and validate similar selection instruments. 相似文献
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Schubert GD 《Journal of forensic sciences》2005,50(6):1411-1416
Many automobile manufacturers began installing airbags on the driver's side in the late 1980's. Passenger side airbags followed in the early 1990's. Most airbags use a solid-propellant type of material that produces a hot gas to inflate the airbags. The gas in the driver side airbag leaves the inflator at a temperature as hot as 600 degrees C. The hot gas escapes through the vent holes after deployment, but it can also leak through the stitching seams in the front and singe a pattern on the occupant's clothing characteristic of the seam pattern. The singe patterns from the driver and passenger side airbags will be different. Cornstarch, which is used as a lubricant in some driver side airbags, can transfer to the driver's shirt. Hairs, fibers and make-up can transfer from the driver or passenger to the surface of the deployed airbags. Two cases are presented, illustrating singe patterns and particle transfers, and how they helped determine who a driver or passenger were. 相似文献
190.
Metal and metalloid multi-elementary ICP-MS validation in whole blood, plasma, urine and hair. Reference values 总被引:5,自引:0,他引:5
Goullé JP Mahieu L Castermant J Neveu N Bonneau L Lainé G Bouige D Lacroix C 《Forensic science international》2005,153(1):39-44
Four multi-elementary metal and metalloid quantification methods using inductively coupled plasma mass spectrometry (ICP-MS) were developed and validated in human whole blood, plasma, urine and hair by means of a single preparation procedure for each sample. The ICP-MS measurements were performed using a Thermo Elemental X7CCT series and PlasmaLab software without a dynamic reaction cell. With this procedure 27-32 elements can be simultaneously quantified in biological matrices: Li, Be, B, Al, V, Cr, Mn, Co, Ni, Cu, Zn, Ga, Ge, As, Se, Rb, Sr, Mo, Pd, Ag, Cd, Sn, Sb, Te, Ba, W, Pt, Hg, Tl, Pb, Bi, U. Whole blood, plasma and urine samples (0.4 ml each) were diluted with purified water, acid, triton X100 and butanol. Rhodium was used as internal standard. The urine sample results were corrected for enzymatic creatinine determination. Twenty-five milligrams hair samples were acid mineralized after a decontamination procedure and diluted as previously described for biological fluids. To be validated, each element had to show linearity with a correlation coefficient higher than 0.99. The intra-assay and inter-assay inaccuracy, measured as the variation coefficient, were below 5 and 10% respectively. Global performance was assessed by a quality control program. Our laboratory is a registered participant of the Institut National de Santé Publique du Québec (Sainte-Foy, Canada) inter-laboratory comparison program for whole blood, urine, and beard hair of non-occupationally exposed individuals spiked with selected elements. In our study multi-element metal and metalloid analysis was assessed for 27 elements in whole blood, 27 elements in plasma, 30 elements in urine and 32 elements in hair, from 0 to 25, or 250 to 1000 ng/ml, depending on the element. Quantification limits ranged from 0.002 ng/ml (U) to 8.1 ng/ml (Al) for whole blood, from 0.002 ng/ml (U) to 7.7 ng/ml (Al) for plasma, from 0.001 ng/ml (U) to 2.2 ng/ml (Se) for urine, and from 0.2 pg/mg (Tl) to 0.5 ng/mg (B) for hair. Normal values were determined in whole blood (n=100), plasma (n=100), urine (n=100), and hair (n=45) of healthy volunteers, leading to approximately 10,000 analyses. All results are presented and discussed. Clinical toxicology and forensic toxicology applications are also reported. ICP-MS has made significant advances in the field of clinical biology, particularly in toxicological analysis. This is due to the use of extremely effective equipment that permits better clinical and forensic toxicological analysis of metal and metalloid status of each individual patient. 相似文献