Detection of orosomucoid 1 phenotypes in semen and semen stains

Orosomucoid 1 phenotypes were detected in seminal plasma by isoelectric focusing and immunoprinting. The orosomucoid 1 phenotypes in seminal plasma correlated with the types found in the corresponding serum specimens. Semen stains stored for ten days could be typed for orosomucoid 1. The present work revealed that orosomucoid 1 is a useful genetic marker for the medicolegal grouping of semen stains.     

Determination of salvinorin A and salvinorin B in Salvia divinorum-related products circulated in Japan

Two major salvinorins, salvinorin A (SalA) and salvinorin B (SalB), in three Salvia divinorum dried leaf products and nine of its “concentrated extract” products circulated in Japan were determined. These ingredients were extracted twice with acetonitrile and decolored with graphite carbon powder. SalA and SalB were confirmed by liquid chromatography–tandem mass spectrometry in product ion scan mode, and quantified by high-performance liquid chromatography with UV detection (for SalA) and by mass spectrometry in single ion monitoring mode (for SalB). The SalA/SalB contents (μg/mg) were in the range of 3.2–5.0/0.10–0.17 in the dried leaf products and 4.1–38.9/0.26–2.42 in the “concentrated extract” products. These findings would be useful for analysis of S. divinorum-related products circulated in the drug market.     

The Effects of Risk Preferences on the Adoption of Post-Harvest Technology: Evidence from Rural Cambodia

We investigate how rice farmers’ risk preferences affect the adoption of post-harvest technology in Cambodia, focusing on moisture meters. We find that risk-averse farmers are more likely to adopt moisture meters, although the degree of farmers’ loss aversion or the extent to which they tended to overweight small probabilities do not affect the adoption. In the setting of our study, the effects of farmers’ risk preferences on agricultural technology adoption can be described by using expected utility theory. However, controlling for loss aversion and probability weighting improves the precision of examining the effects of farmers’ risk preferences on adoption.     

Does the Heterogeneity of Project Implementers Affect the Programme Participation of Beneficiaries?: Evidence from Rural Cambodia

This study employs the dataset collected for the assessment of a post-harvest technology project in rural Cambodia and focuses on the heterogeneous preferences of project implementers, frequently overlooked in the literature on programme evaluation studies. We focus on the ‘implementer effect’ on the programme participation of the treated farmers. We demonstrate that the heterogeneous programme participation of ordinary farmers could be induced due to heterogeneity in the characteristics of the project staff. In particular, we indicate that the baseline altruism of the project staff, measured by the dictator game, consistently increases the participation rate and the number of participations in the training sessions of beneficiaries. This type of heterogeneity in project staffs’ preferences across treatment sites could be a source of treatment heterogeneity for programmes conducted at a certain cluster level. While few studies have focused on the heterogeneity of programme implementers, our empirical results indicate that the preference of implementers could be a source of treatment heterogeneity and imply the importance of implementation of an actual project.     

Pulmonary bone marrow embolic phenomena. Antemortem reactions in traumatic immediate death

This study is based on an examination of lung tissue from 11 cases of traumatic immediate death with fracture. According to our investigation, pulmonary bone marrow embolic phenomena, such as megakaryocytes associated with fat globules in pulmonary arterioles and a megakaryocyte embolism in a pulmonary capillary, develop in most traumatic immediate death cases with fracture. From the results, we suggest that these pulmonary bone marrow embolic phenomena can be certain evidence of antemortem violence in traumatic immediate death cases.     

Molecular analysis of the human esterase D gene ESD(*)Q0(yonago) responsible for incompatibility in a Japanese paternity case

In a Japanese paternity test, an alleged father was excluded only by reverse homozygosity of esterase D (ESD) phenotypes (mother, ESD 1; child, ESD 1; alleged father, ESD 2) out of 43 classical and DNA markers investigated. To solve the aberrant inheritance of the ESD phenotypes observed between them, fragments for all eight coding exons amplified by polymerase chain reaction (PCR) were subjected to DNA analysis. The child and alleged father shared a null allele, originating from ESD(*)1. It was characterized by having TGA for the stop codon instead of TCA for serine at codon 63. Thus, the sharing of a rare null gene, ESD(*)Q0(yonago), increased the probability of paternity.     

Swimming and loss of consciousness

Under certain circumstances, even a good swimmer may drown during swimming exercise. Two cases of drowning, a survivor and a dead, during swimming exercise in swimming-pool are described. These cases and experimental researches with dogs indicate that the initial aspiration of water may cause extremely low heart rate and low blood pressure by reflex vagal inhibition, which deprive a good swimmer of his consciousness and make him drown.     

Development of a Library Search‐Based Screening System for 3,4‐Methylenedioxymethamphetamine in Ecstasy Tablets Using a Portable Near‐Infrared Spectrometer

This is the first report on development of a library search‐based screening system for 3,4‐methylenedioxymethamphetamine (MDMA) in ecstasy tablets using a portable near‐infrared (NIR) spectrometer. The spectrum library consisted of spectra originating from standard substances as well as mixtures of MDMA hydrochloride (MDMA‐HCl) and diluents. The raw NIR spectra were mathematically pretreated, and then, a library search was performed using correlation coefficient. To enhance the discrimination ability, the wavelength used for the library search was limited. Mixtures of MDMA‐HCl and diluents were used to decide criteria to judge MDMA‐positive or MDMA‐negative. Confiscated MDMA tablets and medicinal tablets were used for performance check of the criteria. Twenty‐two of 27 MDMA tablets were truly judged as MDMA‐positive. Five false‐negative results may be caused by compounds not included in the library. No false‐positive results were obtained for medicinal tablets. This system will be a useful tool for on‐site screening of MDMA tablets.     

Do Female Gender Role Attitudes Affect Labour Market Participation in Egypt?

We analyse the inter-generational relationship of attitudes towards gender roles and their impact on individuals’ labour market participations in Egypt. We find a strong correlation between mothers’ and daughters’ perceptions of gender roles. Unlike the existing literature, perceptions are not associated with the labour market status even if we control for endogeneity issues using an instrumental variable regression. Furthermore, higher-educated females are more likely to be unemployed in Egypt. These results imply that perceptions among females are not translated into their labour market behaviour and the supply-side policies alone may not be sufficient for improving young women’s employment opportunities.     

Orosomucoid 1 phenotyping from human urine by isoelectric focusing

Orosomucoid 1 polymorphism was revealed in human urine by isoelectric focusing and immunoprinting on polyacrylamide gels. The orosomucoid in urine samples was recovered and concentrated by using immobilized allo A lectin. The orosomucoid 1 phenotypes of 60 urine samples correlated with those of the corresponding serum samples.