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11.
Investigating the Postmortem Molecular Biology of Cartilage and its Potential Forensic Applications
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Shawna N. Bolton M.Sc. Michael P. Whitehead Ph.D. Jayesh Dudhia Ph.D. Timothy C. Baldwin Ph.D. Raul Sutton Ph.D. 《Journal of forensic sciences》2015,60(4):1061-1067
This study investigated the postmortem molecular changes that articular cartilage undergoes following burial. Fresh pig trotters were interred in 30‐cm‐deep graves at two distinct locations exhibiting dissimilar soil environments for up to 42 days. Extracts of the metacarpophalangeal (MCP) and metatarsophalangeal (MTP) joint cartilage from trotters disinterred weekly over 6 weeks were analyzed by Western blot against the monoclonal antibody 2‐B‐6 to assess aggrecan degradation. In both soil conditions, aggrecan degradation by‐products of decreasing molecular size and complexity were observed up to 21 days postmortem. Degradation products were undetected after this time and coincided with MCP/MTP joint exposure to the soil environment. These results show that cartilage proteoglycans undergo an ordered molecular breakdown, the analysis of which may have forensic applications. This model may prove useful for use as a human model and for forensic investigations concerning crimes against animals and the mortality of endangered species. 相似文献
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F. G. Bolton Jr. 《Journal of family violence》1987,2(1):67-80
Concern regarding the risk of child maltreatment in adolescent parent-child dyads has moderated in recent study. Concern persists, however, in those relationships where demographic or dynamic aspects of the relationship predict greater stress. While the majority of research targeting these high-risk adolescent parent-child dyads has focused on the adolescent mother, there has been little attention directed to the possible role played by the father in the adolescent parenting situation. This paper examines the characteristics of 145 fathers in adolescent mother-child relationships previously determined to be “high-risk” for child abuse. The hypothesis that the fathers in these high-risk relationships may bring elements to the environment and relationship which exacerbate the existing risk (e.g., occupational, educational, and financial problems) was supported. In addition, speculation was off ered that certain personal characteristics of the fathers such as alcohol/substance abuse and criminal records would serve to predict greater rates of ultimate relationship failure. 相似文献
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Ivor Lucas Colin Shindler David Heard Gerd Nonneman Alan Mackie Hugh Arbuthnott 《亚洲事务》2013,44(1):113-127
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