Usefulness of dura mater in providing DNA samples for identifying cadavers

We examined the usefulness of the dura mater in identifying human remains. Dura mater was collected from 50 cadavers, including drowned, charred, and mummified remains. The STR genotype using the AmpFlSTR Identifiler Kit could be typed at 15 STR and amelogenin loci in 30 samples of 33 cases. Furthermore, the ABO genotype and amelogenin using gel-based methods could be typed in 44 samples of 50 cases. In cases with successful typing of STR, ABO-DNA, and amelogenin, the longest time after death was from 12 to 26 days in a drowned body. The minimum quantity of dura mater required for DNA extraction was about 2.5 mg, dried and fixed by ethanol, in a cadaver 15 h after death. The state of the DNA from the dura mater from the calvaria may be better than that from the basis cranii interna. We found that DNA from dura mater is one of the most useful samples for forensic identification.     

Age estimation by measuring the racemization of aspartic acid from total amino acid content of several types of bone and rib cartilage: a preliminary account

Age estimation has been often performed based on the rate of aspartic acid racemization using bones. In this study, we investigated various kinds of bone and a cartilage for suitability to estimate age by racemization. Ten male cadavers aged 22 to 77 years at death were selected, and bone specimens and cartilage were taken from seven sites in each individual. The rate of racemization of aspartic acid among total amino acid contained in each specimen was analyzed by gas chromatography. The correlation coefficient between the rate of racemization and chronological age was relatively high in the sternum, skull, and femur. The rates of aspartic acid racemization were high in the costal cartilage, femur, and skull. In addition, we found that the rate of racemization was only slightly lower after the second irrigation than after the first irrigation in femur and skull bone specimens, but those of others were significantly lower. These findings showed that among the six different bones and rib cartilage, the skull and femur might be used most effectively for age estimation using total amino acid fraction.     

Peer Reactions,Peer Behavior,Student Attitudes,and Student Deviance: a Comparison of College Students in Japan and the USA

This study presents evidence on the cross-cultural generalizability of differential association/social learning theory by testing whether the causal processes of learning attitudes toward deviance, posited by the theory, are equally applicable, and the causal links, specified by the theory, are equally strong in two diverse cultures—the USA and Japan. Drawing on the literature concerning cultural variability in individualism-collectivism, we predicted that the effects of peer reactions to deviance and peer deviance on a person’s attitudes toward deviance should be stronger in Japan than in the USA, and that the mediating effect of a person’s attitudes on the relationship of peer reactions and peer deviance to a person’s deviance should be weaker among Japanese than among Americans. Analyses of comparable survey data from college students in the USA (N?=?625) and Japan (N?=?591) provide generally supportive, but somewhat mixed, evidence regarding our predictions. In both countries, peer reactions to deviance predicted student attitudes toward deviance more strongly than did peer deviance. Peer deviance strongly predicted student deviance, while peer reactions to deviance predicted less strongly, and the effects were mediated by student attitudes in both countries. Contrary to the hypotheses, peer reactions and peer deviance did not predict student attitudes more strongly in Japan than in the USA. Also, peer deviance predicted student deviance more strongly in the USA than in Japan. In agreement with the expectations, the relation between student attitudes and student deviance was stronger in the USA than in Japan.

     

Sudden Death from Cardiopulmonary Arrest on Arrival of a Patient with Pulmonary Tuberculosis: A Case Diagnosed by Postmortem CT and Autopsy

Sudden death due to massive hemoptysis during management of tuberculosis occurs in a considerable number of patients. However, when massive airway hemorrhage occurs in a patient in whom tuberculosis has not been confirmed and a blood is not apparent externally on the face/body, it is difficult to immediately identify the cause of death as airway obstruction by tuberculous bleeding in the airway. We encountered an 83‐year‐old Japanese woman with her medical history included treatment of tuberculosis in her 20s who was in cardiopulmonary arrest on arrival (CPAOA), and the cause of sudden death could not initially be identified. Postmortem CT (PMCT) and autopsy revealed that the cause of sudden death was airway obstruction/asphyxia by tuberculous massive airway hemorrhage. Identification of the cause of death facilitated a subsequent active contact investigation and led to prevention of secondary tuberculosis infection.