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Steinhauer JR Volk A Hardy R Konrad R Daly T Robinson CA 《Journal of forensic sciences》2002,47(1):221-223
Ketosis occurs in ketoacidosis or malnourishment. When either is suspected in relation to a death, it may be important to analyze for ketosis at autopsy. We encountered a case where starvation was suspected in a deceased nursing home resident, where the body had been embalmed prior to autopsy. Gas chromatography (GC) was unable to separate acetone from formaldehyde, a component of embalming fluid. The Acetest is a simple test that can detect acetone and acetoacetate in body fluids. We validated the Acetest with GC on vitreous. The Acetest and GC were consistent except at very low levels of acetone or acetoacetate. The sensitivity of the Acetest for acetoacetate in vitreous was 10 mg/dL, consistent with early starvation. Significant interference from embalming fluid did not occur. The Acetest was negative in the described case. The Acetest is a simple and useful test for the detection of ketosis in embalmed autopsies. 相似文献
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Jon Palfreman 《政策研究评论》2006,23(2):453-472
The suggestion that power line electromagnetic fields (EMFs) cause diseases like cancer has generated dozens of popular articles and television news segments, hundreds of scientific studies, and numerous consensus reports; it has attracted the attention of epidemiologists, biologists, physicists, policymakers and lawyers. This article will examine the evolution of this controversy through a detailed analysis of the arguments that have been used for and against the hypothesis that power line EMFs have adverse health effects. This article argues that the power line EMF issue provides a classic case study for exploring the challenges citizens, scientists, and policymakers face in sorting out a complex science‐based controversy. This story not only brings together many different perspectives and—from popular notions of cancer clusters to complex epidemiological arguments, from to state‐of‐the‐art animal studies to policy instruments such as the precautionary principle—but also reveals the manner in which a heated controversy can be effectively resolved over time. 相似文献
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Jon B. Gould 《Public administration review》2002,62(S1):74-79
The aftermath of September 11th has seen a worrisome rise in invasive surveillance measures. Both adopted by statute and initiated by agencies, these provisions provide unprecedented powers for government agents to investigate suspects and search individuals, whether they are directly involved in terrorism or not. The prevailing wisdom has been that the American people will accept these restrictions as the natural cost of heightened security, and initial evidence suggests the public has been willing to tolerate greater limits on civil liberties. However, over time such support will erode, leaving in place permanent restrictions on civil liberties that not only will concern Americans, but also may turn them against government officials and civic participation. Thus, contrary to many interpretations of September 11th, this article argues that the policy response has only sown the seeds for greater detachment from and dissatisfaction with government as the public becomes increasingly separated from the workings and operations of public policy. 相似文献
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Curbing Corruption: The Elusive Search for a Cure 总被引:1,自引:0,他引:1
Jon S. T. Quah 《Public administration review》2006,66(6):939-943
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During recent years, more state and local governments have made provisions in their budgets for establishing and maintaining contingency reserve funds. A recent study by the National Conference of State Legislatures(NCSL) found that over half of all states now have such funds. The basic purpose of these contingency reserve funds, also referred to as "rainy day" funds, is to insulate governmental budgets from unexpected fiscal disruptions brought about by such factors as unanticipated revenue shortfalls and expenditure overruns. What have received less attention, however, are the criteria which governments need to use in deciding whether to establish such contingency reserves in the first place, and if so, how large these reserves should optimally be. This article explores these two issues, using the State of California as an illustrative case study 相似文献
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Validation of a 21-locus autosomal SNP multiplex for forensic identification purposes 总被引:8,自引:0,他引:8
Dixon LA Murray CM Archer EJ Dobbins AE Koumi P Gill P 《Forensic science international》2005,154(1):62-77
A single nucleotide polymorphism (SNP) multiplex has been developed to analyse highly degraded and low copy number (LCN) DNA template, i.e. <100 pg, for scenarios including mass disaster identification. The multiplex consists of 20 autosomal non-coding loci plus Amelogenin for sex determination, amplified in a single tube PCR reaction and visualised on the Applied Biosystems 3100 capillary electrophoresis (CE) system. Allele-specific primers tailed with shared universal tag sequences were designed to speed multiplex design and balance the amplification efficiencies of all loci through the use of a single reverse and two differentially labelled allele denoting forward universal primers. As the multiplex is intended for use with samples too degraded for conventional profiling, a computer program was specifically developed to aid interpretation. Critical factors taken into account by the software include empirically determined extremes of heterozygous imbalance (Hb) and the drop-out threshold (Ht) defined as the maximum peak height of a surviving heterozygous allele, where its partner may have dropped out. The discrimination power of the system is estimated at 1 in 4.5 million, using a White Caucasian population database. Comparisons using artificially degraded samples profiled with both the SNP multiplex and AMPFISTR SGM plus (Applied Biosystems) demonstrated a greater likelihood of obtaining a profile using SNPs for certain sample types. Saliva stains degraded for 147 days generated an 81% complete SNP profile whilst short tandem repeats (STRs) were only 18% complete; similarly blood degraded for 243 days produced full SNP profiles but only 9% with STRs. Reproducibility studies showed concordance between SNP profiles for different sample types, such as blood, saliva, semen and hairs, for the same individual, both within and between different DNA extracts. 相似文献