14岁青少年手腕骨发育的研究I．男生骨龄标准的制定

目的研究我国当前青少年骨发育状况,制订专用于法医学推断男性刑事责任年龄14岁的骨龄标准。方法以湖南冷水江地区青少年为对象,按照纳入标准,选取14岁±3个月的健康男性中学生103名,拍摄其左手腕部后前位X线片。选择手腕骨15个部位,按照统一标准进行观察、测量,采用最大百分比法确定发育等级标准,建立骨龄评估方法,以单盲法进行验证。结果除第一掌骨、近节第四指骨骨骺发育等级为部分闭合外,其他均为未闭合;对于第四、五掌骨和近三指骨,不足14岁组以未闭合为主,已满14岁组以部分闭合为主。各个观察部位骨骺横径宽于干骺横径。30例的验证结果准确率为80.0%。结论根据发育等级特征及测量学方法建立骨龄评估标准可行,可望得到推广。本文以刑事责任年龄14周岁为对象,制订法医学专业骨龄推断标准,并引入测量学方法,辅助推断年龄。     

浅谈视频监控技术在现场勘查中的作用

视频监控技术是公安机关打击和防控违法犯罪常用的一种技术手段,在刑事现场勘查中也有着举足轻重的作用。正确运用视频监控技术,可以明确现场勘查取证重点,可以有效采取现场紧急措施,可以快速甄别现场痕迹物证,可以提高侦查实验结果的可靠性。     

微小隐孢子虫类钙调蛋白基因真核表达质粒的构建及在Hela细胞中的表达

为了构建微小隐孢子虫类钙调蛋白(calmodulin-like protein,CML)基因的真核表达质粒,并在Hela细胞中实现表达,以微小隐孢子虫卵囊cDNA为模板,通过PCR扩增CML基因,插入到克隆载体pMD18-T中。对经鉴定的pMD-CML重组质粒进行双酶切,将目的基因连接到经同样内切酶双酶切的真核表达载体pVAX1上。重组质粒经双酶切分析和测序鉴定后,用FuGENE HD转染试剂介导的方法,将重组表达质粒转染Hela细胞,用Western-blot技术和间接免疫荧光法检测外源基因的表达。结果显示,成功构建了微小隐孢子虫CML基因的真核表达质粒pVAX-CML,重组质粒在Hela细胞中实现了表达,表达产物具有良好的反应原性,为研究CML的特性和功能、寻找新的防控技术奠定了基础。     

公共服务:行政诉讼群体性纠纷化解新视野——以GT两级法院相关案件为分析样本

行政诉讼群体性案件涉及人数较多、涉及面较广、涉及利益较为复杂,而且表现出较强的有组织性,有的案件甚至表现出对抗性较强、矛盾容易激化的特点,可能存在影响社会稳定的风险。行政诉讼群体性案件已经成为当前行政审判工作的重点和难点,应当引起高度重视,妥善处理该类案件对维护社会稳定至关重要。在行政诉讼群体性案件中,应当依法保障人民群众的合法权益,监督行政机关依法行政;同时,应防范化解相应的维稳风险,发挥行政审判化解矛盾、防范风险、维护稳定的社会治理职能作用。     

A closer look at risk factors for public-private partnerships in Singapore: six case studies

ABSTRACT

Despite its global popularity over the past few decades, the public-private partnership (PPP) has not always led to successful outcomes, due largely to a number of risk factors associated with the projects. To explain how and why PPPs sometimes fail, this study considers the success-failure continuum of Singapore’s recent PPP experience from 2000 to 2019. After taking a critical, close look at the six failed cases, we identify the following latent risk factors: unstable financial capacity during the execution period of a project, force majeure unforeseen problems that arise, a lack of technical and/or financial foresight, poor corporate management (e.g. delays in construction and poor-quality service delivery), and an unfavourable investment environment stemming from the lack of a clear and supportive governance framework. In addition, we find that most risk factors tend to appear during the contract management (pre-operation) and project management (operation) phases. Such risks seem to drive the operational failure and subsequent contract termination of multiple unsuccessful PPPs, simultaneously (and sometimes sequentially) rather than in isolated fashion. All in all, this study offers for policymakers that better risk allocation and proper, mutual coordination between the public and private partners represent essential factors for PPP success.     

Vulnerabilities in China’s Legal System

Social Justice Research -     

猪圆环病毒2型ORF2基因插入猪细小病毒VP2基因3'端对病毒样颗粒形成的影响

以猪细小病毒(PPV)四川分离株SC-1的VP2基因3'端为靶点,在第1215和1216位之间插入长约700 bp的猪圆环病毒2型(PCV2)ORF2基因片段,将重组后的PPV VP2-PCV2 ORF2克隆到真核表达载体pEGFP-C1中,构建了重组质粒pEGFP.VO;经酶切和PCR鉴定后,采用脂质体介导法将重组质粒pEGFP.VO转染COS-7细胞,应用倒置荧光显微镜、电镜、免疫荧光以及生物信息学技术对表达产物进行观察分析.结果显示,重组质粒pEGFP.VO在COS-7细胞中得到表达,但在电镜下没有观察到病毒样颗粒.通过对PPV衣壳蛋白的三维结构分析及免疫荧光检测发现,PCV2 ORF2基因的插入位点位于PPVVP2基因的3'端,相应表达产物则位于VP2蛋白多肽的C端,且被其包裹在内,这可能影响了病毒样颗粒的形成,表明,PPV VP2基因3'端不适合外源基因插入构建重组病毒样颗粒.     

Politikwandel und seine Messung in der vergleichenden Staatstätigkeitsforschung: Konzeptionelle Probleme und mögliche Alternativen

Policy change is an important concept in comparative policy analysis. Despite its central significance, most empirical studies fail to provide clear-cut definitions and measurement of this concept. Against this background, this article evaluates previous scholarship on policy change in the areas of social and environmental policy. We find that most studies use proxies for measuring policy change even though they contradict the basic idea of policy-making activities. Furthermore, studies usually neither capture the complexity of policy change, nor take into the possibility of policy change through dismantling account. Additionally, the empirical focus of most analyses is too narrow, thereby impeding robust statements about causality. In response to these shortcomings, we propose a new conceptual perspective, which captures policy change as a broader empirical phenomenon. We discuss its advantages as well as its disadvantages and show the implications for the research process.     

Does price really converge among provinces in Canada？ A study using unit root tests for cross-sectional panels

This paper examines price convergences across different provinces in Canada using 1983-2006 data. We adopt the new panel unit root tests for analysis that can correct for cross-sectional correlation. Our findings are contrary to previous studies and we conclude that intra-national price convergence does not occur. We also briefly discuss the reasons for the absence of convergence within Canada.