The peacekeeping capacities of African regional organisations

This paper assesses the various peace and security mechanisms that African regional organisations are establishing and other measures that they are taking to enhance their preparedness. In the mid-1990s, the United Nations (UN) Security Council responded to the widely perceived failures of several UN peacekeeping operations by encouraging regional arrangements and agencies to assume a greater role in the promotion of peace and security. As of December 2001, four African organisations had authorised 17 peacekeeping missions. Most of them have been beset by serious and sustained operational and political shortcomings. Recognising their limitations and the vacuum created by Security Council inaction, these and other organisations have undertaken various initiatives to improve on past performance and to prepare for future engagements. A review of their decision-making processes, staffing, mission planning and support, peacekeeping training and financial resources suggests that, while they have made some progress, most organisations are still far from being able to take on the responsibilities that the international community would like them to assume.     

Development of a human-specific real-time PCR assay for the simultaneous quantitation of total genomic and male DNA

A duplex real-time quantitative PCR assay was developed for forensic DNA analysis, which provides simultaneous quantitation of total genomic human DNA and human male DNA. The assay utilizes two spectrally resolved fluorogenic probes in a 5' nuclease (TaqMantrade mark) assay. Within the range of organisms empirically tested and based upon theoretical specificity using National Center for Biotechnology Information GenBank sequences, primer and probe sequences were shown to be human specific, and the Y-chromosome probe, male-specific. A mixture-challenge study resulted in accurate quantitation of 25 pg male DNA in a mixture of up to 1:5000 (male:female DNA). Additional experimental results include comparisons with the slot blot method and commercial real-time PCR kits. The assay developed addresses the shortcomings of the traditional slot blot method as well as the commercial real-time PCR kits. This method is shown to be specific, relatively simple, rapid, has low limits of detection, and consumes limited sample in addition to reporting both the male and total genomic DNA concentrations present.     

Authors Index Volume 10

Authors Index

Authors Index Volume 10