Working at the intersection—a story from Australia

Conceived by nurses in the hospital of a Palestinian refugee camp in Beirut, and inspired by Norwegian People's Aid, the international aid agency of the Australian trade unions was designed to give a genuine material base to solidarity with national liberation struggles. Bridging the difficult division in Australian labour politics between the Catholic right and the social democratic and pro‐Moscow lefts, Australian People for Health, Education and Development Abroad (now Union Aid Abroad APHEDA) was able to channel funds from unions and the Australian government to agriculture, health, and vocational training projects in many countries in the South. Unlike most counterpart organisations in Europe and the USA, its earlier partners were rarely trade unions. Only recently has APHEDA directly supported trade union training in Cambodia, East Timor, and Indonesia, under pressure from Australian unions, who see workers' rights in neighbouring countries as crucial to their own fate. Yet unions in advanced capitalist countries don't spontaneously understand the humanitarian and development needs of countries, such as Papua New Guinea, where waged workers are a small minority of the population. Unionisation is only one part of the solution. The April 2000 Durban congress of the ICFTU called for trade unions to ‘organise the unorganised’, such as informal‐sector workers, and to build alliances with NGOs and civil society around shared values. As a trade union NGO, APHEDA is located in the middle of a challenging intersection. Mandated to educate Australian workers on globalisation issues, APHEDA finds itself often more partisan than other international development NGOs in Australia, sometimes more circumspect. With attacks on union rights and the increasing share of the Australian aid budget delivered through private companies, APHEDA faces decisions about its independence, alliances, direction, and sustainability.     

Detection of thyroglobulin in blood stains as an aid in the diagnosis of mechanical asphyxia

Mechanical forces applied to the neck region are known to release certain amounts of thyroglobulin into circulation. In this experiment, an attempt was made to detect thyroglobulin in bloodstains as an aid in the diagnosis of mechanical asphyxia. Experimental bloodstains containing thyroglobulin at concentrations of 1, 2, 5 and 10 mu g/mL were prepared on a sheet of filter paper. Small pieces of bloodstains, measuring approximately 2.4 cm2 in area, were extracted with 0.1 mL of distilled water and the extracts were tested against an antihuman thyroglobulin serum by precipitation-electrophoresis. Bloodstains containing more than 1 mu g/mL of thyroglobulin formed distinct precipitin lines for up to one month of storage, while bloodstains containing more than 5 mu g/mL of thyroglobulin formed distinct precipitin lines for up to three months of storage. The present results suggest that the bloodstains can be utilized in the diagnosis of mechanical asphyxia.     

A sensitive enzyme-linked immunosorbent assay used to differentiate type AB specimen from a mixture of type A and type B specimens

A sensitive enzyme-linked immunosorbent assay (ELISA) for detection of ABO blood substances in human body fluids was devised. The ELISA plates coated with purified human anti-A or anti-B serum effectively captured the blood substances, and these were then analysed by the combination of rabbit anti-A and goat anti-B. This capture ELISA could differentiate the type AB specimen from a mixture of the type A and the type B specimens, and the method was applied to rape cases to make the ABO typing of the criminal.     

Green pigments in epidermal blisters of decomposed cadavers

A spectrophotometrical method for discrimination between sulfhemoglobin (SHb) and choleglobin was applied to analyses of the green pigments in epidermal blisters of decomposed cadavers. SHb appeared to be a predominant constituent of the pigments, although significant amounts of choleglobin, one third to one sixth of SHb, were also demonstrated. The present results indicate that the greenish discoloration of decomposed cadavers is due to formation of a mixture of SHb, choleglobin and possibly other denatured hemoglobin derivatives.     

Rapid isolation with Sep-Pak C18 cartridges and capillary gas chromatography of triazine herbicides in human body fluids.

A simple and rapid method, for the isolation of eight triazine herbicides from human serum and urine, using Sep-Pak C18 cartridges is presented. After mixing with distilled water, serum and urine samples containing the herbicides, were loaded on Sep-Pak C18 cartridges and eluted with either chloroform only or chloroform/methanol (9:1). The herbicides were detected by capillary gas chromatography with both flame ionization detection (FID) and nitrogen-phosphorus detection (NPD). Separation of eight triazine herbicides from each other and from impurities was generally satisfactory with the use of a non-polar DB-1 capillary column. Recovery of most compounds was excellent for both chloroform and chloroform/methanol (9:1) as elution solvents. Backgrounds were cleaner and evaporation time was shorter for the chloroform only than for the chloroform/methanol (9:1). The NPD gave sensitivity more than 10-20 times higher than that of FID.