Response of short tandem repeat systems to temperature and sizing methods

In capillary gel electrophoresis (CE), changing run conditions such as temperature can result in minor variations in the size determination of an allele. These effects are caused by secondary structure differences that can occur between the amplified sample and the internal standard. The type of method chosen to generate the sizing curve in STR analysis can influence the relationship between estimated allele size and temperature. To better understand the effects of temperature and sizing method on the reproducibility of DNA migration, two fluorescently labeled allelic ladders, CTTv and Y-PLEX 6 were analyzed using the ABI Prism 310 Genetic Analyzer. The default method on the Genetic Analyzer utilizes an electrophoretic temperature of 60 degrees C and a Local Southern method to generate a sizing curve from the fragment migration times of the internal lane standard. In this work, electrophoresis was conducted at 35-70 degrees C using the commercially available POP 4 buffer at pH 8 and two sizing methods, Global Southern and Local Southern, were compared. The slopes of the regression line between estimated allele size and temperature, using either sizing method, were measured in order to demonstrate the temperature sensitivity of migration time and the importance of the operator-chosen method. Our results indicate that the Global Southern method is a better choice in situations where temperature fluctuations can occur. In addition, the temperature dependence of the DNA size estimates using the POP 4 system were compared to results obtained using an experimental buffer consisting of 3% hydroxyethylcellulose at pH 11. These results demonstrate that secondary structure effects are minimized at an elevated pH, increasing the precision of size estimates obtained.     

Guys and Dolls

The doll has become a figure for the objectification of women, especially in the form of the sex doll, which is routinely taken to be the image of the woman reduced to a condition of pure passivity. But the doll is also, and perhaps even predominantly, a means of ‘guying’ the putative man (the word ‘guy’ entering English with the burning of Guy Fawkes in effigy) and presenting the simplest possible idea of male desire in comically petrified form. Effigies of women indirectly figure the assumed fixation of male desire throughout post-classical European culture. This guying is a repeated feature of stories of the animation of female dolls and statues, from Ovid onwards, which regularly put male protagonists to a kind of mock death, or death by mockery. The author considers the anonymous poem Adollizing: Or, A Lively Picture of Adoll-Worship (1748) alongside the most celebrated instance of doll fetishism in twentieth-century art history—the doll that Oskar Kokoschka had made of his wife Alma Mahler after she left him. The author concludes with a discussion of the contemporary sexual cult of ‘living dolls’, in which men act out the fantasy not of owning, but being a female doll. Here, perhaps, the doll is not only adored as a substitute for some other real object, but is envied as what it is—that is, as a thing, with a thing's power of declining to be subjected to subjecthood. This passion for passivity suggests that there is something that intervenes between the he and she, with swelling erotic force: the it.     

Missing Masculinities: Gendering Practices in Australian Alcohol Research and Policy

The issue of ‘alcohol-fuelled violence’ has been the subject of intense policy debate in Australia. While this debate is warranted, its contours and content have been informed and shaped by a surprisingly narrow range of research resources. Narrow research engagements of this kind warrant scrutiny because they can exclude from consideration crucial issues. In this article we identify one such issue, that of gender. Following a review of the Australian literature on gender, alcohol and violence, our analysis explores four case studies drawn from the Australian research corpus, focusing on large quantitative studies as these tend to receive most attention and citation in policy debate. Such studies consistently erase the contribution of key gender dynamics, namely enactments of particular (often youthful) masculinities, to violence involving alcohol, even where they simultaneously provide strong support in their data for such a conclusion. We show how this research is mobilised specifically in support of claims about the causal role of alcohol in violence and of blanket population-level responses to the problem. There is an urgent need to map the character and scope of the tendency to erase certain gender issues in research on alcohol and violence in order to better inform policy responses.     

Pathways to Institutional Legitimacy in Postconflict Societies: Perceptions of Process and Performance in Nepal

Rebuilding institutional legitimacy is considered essential for stability in postconflict societies, yet the factors that influence citizen perceptions of legitimacy in this context remain underresearched. In this article, we examine citizen evaluations of government legitimacy in terms of instrumental antecedents (service delivery, distributive justice) and procedural antecedents (procedural justice, voice), using data collected in the context of a nationwide study of postconflict governance in Nepal. We find that procedural justice is more strongly associated with citizen perceptions of institutional legitimacy than instrumental outcomes such as service delivery, distributive justice, and outcome favorability. Results indicate that the relationship between service delivery and legitimacy is not as simple as previously assumed. We conclude that procedural justice is crucial for building perceptions of government legitimacy in postconflict societies and discuss implications for policy and practice relating to postconflict governance and institutional trust building.     

An integrated exploration of factors associated with psychological distress among older prisoners

This paper compares the contributions of socio-demographic, health, mental health, social and environmental factors in explaining variations in psychological distress among older prisoners in two Australian states. One hundred and seventy-three prisoners (aged 50+ ) from 8 Australian prisons were interviewed using the Kessler Psychological Distress (K10) Scale. Three regression analyses were conducted to compare different models of 14 variables significantly associated with psychological distress. Independent variables most prominently associated with variations in psychological distress among older inmates were self-reported levels of social support, self-reported safety and ease of health care access. Difficulties in the built environment and mental health history were significantly explanatory of variations in older prisoner distress in two of the three models. The findings suggest that modifiable situational factors evident in current prison contexts are reasonably explanatory of variations in prisoner distress among older inmates.     

Identifying Predictors for Children Witnessing Intimate Partner Violence

Research has shown that intimate partner violence (IPV) prevalence and severity is higher and IPV duration is longer among couples that have children. Women frequently report that their children are the reason why they stay, leave, or return to an IPV relationship. Our study used results from a two-wave telephone survey to determine what IPV-associated factors were significant predictors of respondents’ children witnessing IPV, as well as estimating prevalence of children’s exposure to violence. We found that an increase in respondents’ age was significantly associated with increased odds of a child being exposed to violence. We also found that children witnessing violence were almost twice as likely to have mothers who reported leaving abusers. We hypothesize that increasing age corresponds to improved confidence in help-seeking behaviors. Our findings represent an important first step for future research on understanding how children influence IPV victims’ decision-making in seeking out service providers for help.     

Police interviews with suspected child sex offenders: does use of empathy and question type influence the amount of investigation relevant information obtained?

The use of the criminal justice system to force offenders to receive psychological treatment is one of the most controversial aspects of service provision for offenders. Coerced treatment needs to be distinguished from pressured treatment, both having objective and subjective dimensions. In this paper some arguments for and against coerced offender rehabilitation are discussed. We suggest that coercing offenders into attending rehabilitation programmes (or placing legal pressure on them to attend) is unlikely by itself to lead to poorer outcomes. Rather, the individual's perception of coercion will be more influential in determining how an offender approaches treatment. Even when offenders perceive they are being coerced, it is likely that pre-treatment anti-therapeutic attitudes can change over the course of a programme, such that therapeutic gains (risk reduction) can occur. Coercion and its effects on treatment engagement and rehabilitation outcomes require further empirical research and conceptual analysis.     

The impact of “empirical facts” on legal scholarship and legal research training

Lawyers have traditionally viewed law as a closed system, and doctrinal research has been the research methodology used most widely in the profession. This reflects traditional concepts of legal reasoning. There is a wealth of reliable and valid social science data available to lawyers and judges. Judges in fact often refer to general facts about the world, society, institutions and human behaviour (“empirical facts”). Legal education needs to prepare our students for this broader legal context. This paper examines how “empirical facts” are used in Australian and other common law courts. Specifically, the paper argues that there is a need for enhanced training in non-doctrinal research methodologies across the law school curriculum. This should encompass a broad introduction to social science methods, with more attention being paid to a cross-section of methodologies such as content analysis, comparative law and surveys that are best applied to law.     

Testing the Use of Pigs as Human Proxies in Decomposition Studies

Pigs are a common human analogue in taphonomic study, yet data comparing the trajectory of decomposition between the two groups are lacking. This study compared decomposition rate and gross tissue change in 17 pigs and 22 human remains placed in the Forensic Investigation Research Station in western Colorado between 2012 and 2015. Accumulated degree days (ADD) were used to assess the number of thermal units required to reach a given total body score (TBS) (1) which was used as the measure of decomposition. A comparison of slopes in linear mixed effects model indicated that decomposition rates significantly differed between human donors and pig remains χ²₍₁₎ = 5.662, p = 0.017. Neither the pig nor the human trajectory compared well to the TBS model. Thus, (i) pigs are not an adequate proxy for human decomposition studies, and (ii) in the semiarid environment of western Colorado, there is a need to develop a regional decomposition model.