Microbiota Composition and Pulmonary Surfactant Protein Expression as Markers of Death by Drowning

Pathological diagnosis of drowning remains a challenge for forensic science, because of a lack of pathognomonic findings. We analyzed microbiota and surfactant protein in the lungs for a novel diagnosis of drowning. All rats were divided into drowning, postmortem submersion, and control groups. The water, lungs, closed organs (kidney and liver), and cardiac blood in rats were assayed by targeting 16S ribosomal RNA of Miseq sequencing. Lung samples were analyzed by immunohistochemical staining for surfactant protein A. The closed organs and cardiac blood of drowned group have a lot of aquatic microbes, which have not been detected in postmortem submersion group. Furthermore, intra‐alveolar granular staining of surfactant protein A (SP‐A) was severely observed in the drowned group than the postmortem submersion and control groups. The findings suggested that the presence of aquatic microbiota in the closed organs and increased expression of SP‐A could be markers for a diagnosis of drowning.     

Optimization of Headspace Solid‐Phase Microextraction Technique for Extraction of Volatile Smokeless Powder Compounds in Forensic Applications

Smokeless powders are low explosives and are potentially found in cases involving firearms and improvised explosive devices. Apart from inorganic compound analysis, forensic determination of organic components of these materials appears as a promising alternative, especially the chromatographic techniques. This work describes the optimization of a solid‐phase microextraction technique using an 85 μm polyacrylate fiber followed by gas chromatography‐flame ionization detection for smokeless powder. A multivariate experimental design was performed to optimize extraction‐influencing parameters. A 2⁴ factorial first‐order design revealed that sample temperature and extraction time were the major influencing parameters. Doehlert matrix design has subsequently selected 66°C and 21 min as the compromised conditions for the two predetermined parameters. This extraction technique has successfully detected the headspace compounds of smokeless powders from different ammunition types and allowed for their differentiation. The novel technique allows more rapid sample preparation for chromatographic detection of smokeless powders.     

The political economic factors of migration to Bangkok

A new way of urbanization has emerged in China where farmers are urbanizing the rural areas instead of migrating to the large cities. This article addresses the question of why current urbanization takes this direction in which surplus labor has been transferred from agriculture into the industrial and service sectors without leaving the rural areas. The findings indicate that continual population pressure and reform policies in post-Mao China have resulted in strategic development of small towns in the rural areas. In this development, township and village enterprises have been the locomotive of rural urbanization. Institutional constraints could be moved, created, or utilized in a way which can be conductive for social development. The rural urbanization in China could be significant for many developing countries.     

What Money Can’t Buy: The Security Externalities of Chinese Economic Statecraft in Post-War Sri Lanka

ABSTRACT

China’s growing trade, investment, and aid links are commonly believed to constitute a potent instrument of statecraft, generating important security externalities. Yet there is insufficient research tracing the precise mechanisms linking economic relationships between a “sender” and “target” state to actual influence in the security domain. We offer three contributions. First, we map out the theoretical mechanisms of influence in a sender–target relationship. Second, we empirically investigate these mechanisms through a case study of China’s economic influence in Sri Lanka since 2009. Third, we use our findings to generate new insights on the mechanisms of influence in the economic statecraft literature and the dynamics of great-power competition in South Asia. Beijing’s ability to convert its considerable economic resources into strategic influence in Sri Lanka is currently hampered by the poor planning and implementation of infrastructure projects, domestic politics, and Sri Lanka’s relationship with India, a regional competitor and rising power.     

Statistical Evaluation of Alternative Light Sources for Bloodstain Photography

Bloodstain photography is important in forensic applications, especially for bloodstain pattern analysis. This study compares the enhancement effect of bloodstain photography using three different types of light source: fluorescent white light, near‐ultraviolet (UV) light‐emitting diode (LED) light, and 410 nm LED light. Randomized complete block designs were implemented to identify the lighting that would statistically produce the best enhancement results for bloodstains on different types of surfaces. Bloodstain samples were prepared on white cotton, brown carpet, tar road, and wood. These samples were photographed in darkroom conditions using a Canon EOS 50D digital SLR camera, with Canon EFS 60 mm f/2.8 Macro USM lens. Two‐way analysis of variance and Fisher's least significant difference test were used to analyze the contrast of the images. The statistical analysis showed that 410 nm light is the best among the tested lights for enhancing bloodstains on the tested surfaces, where the contrast of bloodstain to background was the highest.     

Gang affiliation,aggression, and violent offending in a sample of youth offenders

Abstract

Gang affiliation, aggression, and violent offending were examined in case files of 390 youth offenders aged between 16 and 18 years. Results indicated that youth offenders who were gang members and those who were not gang members but exposed to friends in gangs had a significantly higher likelihood of violent offending compared with a reference group of youth offenders who had neither gang affiliation nor friends in gangs. Additionally, youth offenders who had friends in gangs but were themselves not gang members had a lower likelihood of violent offending than youth offenders who were gang members. Finally, results showed that a history of aggressive behavior was significantly associated with violent offending. Implications such as the need to address the influence of delinquent peers and need to address the management of anger and aggression in youths will be discussed. Also, findings point towards the need for prevention and early intervention work.     

Crossing the river that divides the Korean Peninsula: an evaluation of the Sunshine Policy

This paper sheds light on aspects of functionalism and neo-functionalismin the Sunshine Policy and evaluates its contribution to inter-Koreanrelations through statistical analyses. On the whole, the policycould increase the trade volume and diversify trade structuresencompassing primary and secondary industries, as well as processing-on-commission-basedtrade in economic sectors. Our research results demonstratethat the policy has contributed to the progress of inter-Koreanrelations in socio-economic areas. Notwithstanding the increasingeconomic gap between the North and South, inter-Korean tradehas expanded in the context of the economic urgency of the Northand the mutual benefits of inter-Korean cooperation. In socialsectors, the Kim Dae Jung government in comparison to previousgovernments encouraged personal exchanges, and these personalcontacts should enhance mutual understanding between the Northand South Korean people. In political and military sectors,the Sunshine Policy has been less positive in the current militarybuild-up by both Koreas. Since the security issue of the twoKoreas is the most vital factor for survival, broad and long-termperspectives based on mutual reliance and trust are needed.     

Standardization of method for the analysis of dextromethorphan in urine

Dextromethorphan (DMP), an antitussive, is one of the most popular drugs among the younger generation in Korea. It usually is taken for its hallucinogenic properties and overdoses have been responsible for the fatalities that have been reported frequently. To control the abuse of DMP, the authorities restricted its use through classifying it as a controlled drug on October 2003. The purpose of this study is to provide a standard method for the analysis of DMP and its main metabolite, dextrorphan (DTP) in biological specimens. At first we established a standard operating procedure (SOP) for DMP/DTP in urine, and a method validation was performed. We also quantified DMP from 16 drug abuser's urine samples all of which were positive in the screening test for DMP. For the detection of DMP/DTP, urine samples were adjusted with 6N NaOH (pH 11) and extracted with ethylacetate. Thin layer chromatography was used as the screening test, and the final identification for DMP/DTP was used by GC/MS. The ions (m/z 271 for DMP, m/z 257 for DTP and m/z 86 for lidocaine as internal standard) were extracted from the full scan mass spectrum and were used for quantification. The selectivity, linearity of calibration, accuracy, within- and between day precision, limit of detection and quantification, recovery and stability were examined as parts of the method validation. Extracted calibration curves were linear from 100 to 2000 ng/mL for DMP and DTP with correlation coefficients better than 0.999. Limit detection was 50 ng/mL for DMP and DTP. Within-run precision (%CV) for DMP and DTP at three different concentrations (100, 500 and 1000 ng/mL) was 6.10-18.85%, and between-run precision was 1.70-7.86% for DMP and DTP. Absolute recovery for DMP and DTP was 57-74%, and relative recovery (extraction efficiency) was 80-89%. For 16 drug abuser's urine samples, the concentrations of DMP and DTP were 0.16-52.63 and 0.41-23.75 microg/mL, respectively. Method validation is an important requirement in the practice of chemical analysis, and it will be particularly useful in verifying the reliability of analytical results in the field of forensic science.