Analyzing Brazilian vehicle documents for authenticity by easy ambient sonic-spray ionization mass spectrometry

Using desorption/ionization techniques such as easy ambient sonic-spray ionization mass spectrometry (EASI-MS), it is possible to analyze documents of Brazilian vehicles for authenticity, providing a chemical profile directly from the surface of each document. A method for the detection of counterfeit documents is described, and the falsification procedure is elucidated. Forty authentic and counterfeit documents were analyzed by both positive and negative ion modes, EASI(±)-MS. EASI(+)-MS results identified the presence of (bis(2-ethylhexyl)phthalate plasticizer and of dihexadecyldimethylammonium biocide in both types of documents. For EASI(-)-MS results, the 4-octyloxybenzoic acid additive ([M + H](+): m/z 249) is present only in counterfeit documents. It was also found that counterfeit vehicle documents are produced via Laserjet printers. Desorption/ionization techniques, such as EASI-MS, offer therefore, an intelligent way to characterize the counterfeiting method.     

Demystifying "oxi" cocaine: Chemical profiling analysis of a "new Brazilian drug" from Acre State

Recent information from various sources suggests that a new illicit drug, called "oxi", is being spread across Brazil. It would be used in the smoked form and it would look like to crack cocaine: usually small yellowish or light brown stones. As fully released in the media, "oxi" would differ from crack cocaine in the sense that crack would contain carbonate or bicarbonate salts whereas "oxi" would include the addition of calcium oxide and kerosene (or gasoline). In this context, this work presents a chemical profiling comparative study between "oxi" street samples seized by the Civil Police of the State of Acre (CP/AC) and samples associated with both international and interstate drug trafficking seized by the Brazilian Federal Police in Acre (FP/AC). The outcome of this work assisted Brazilian authorities to stop inaccurate and alarmist releases on this issue. It may be of good use by the forensic community in order to better understand matters in their efforts to guide local law enforcement agencies in case such claims reach the international illicit market.     

Comparison of methods to determine sex by evaluating the greater sciatic notch: Visual, angular and geometric morphometrics

Sex estimation is the first step for biological profile reconstruction of an unknown skeleton (archaeological or contemporary) and consequently for positive identification of skeletal remains recovered from forensic settings. Several tools have been developed using different osseous structures. With the intention to provide an objective method comparison, we reported the analysis of three different methods (visual, metric and geometric morphometrics) for sex assessment of the greater sciatic notch. One hundred and thirty pelvic bones (45.4% females and 54.6% males) from the National Autonomous University of Mexico Skeletal Collection pertaining to the contemporary Mexican population were analyzed. We used the ROC-analysis to test between desired false positive thresholds (1-specificity) and expected true positive rates (sensitivity) in order to predict the best approach to sex assessment. The comparison of the area under the ROC-curves shows significant differences among visual and metric methods. At the same time, the analysis suggested that higher morphological variation among the sexes is independent of the methodological approach. The results indicate that the metric (angle), with a high percent of indeterminate cases (34.6%), and visual, with 26.2% of the cases allocated as intermediate cases, were poorly accurate; we cannot recommend these techniques for sexing an unknown specimen. On the other hand, the geometric morphometrics approach improves sex estimation in 82.3% of correctly classified individuals with more than 95% of posterior probability. In addition to the method comparison, the major sexual variation of the greater sciatic notch was determined to be located on its posterior border.     

Why is methenamine detected in Goma-2 dynamites originally methenamine free? An interpretation of relevant forensic results

After the train bombing in Madrid (Spain) on 11 March 2004, methenamine was detected in some of the specimens of Goma-2 ECO dynamite submitted to the forensic laboratories when analyzed by gas chromatography coupled with mass spectrometry detection (GC-MS). Methenamine is synthesized from formaldehyde and ammonia through a condensation reaction. However, neither methenamine nor any of these compounds were used to manufacture Goma-2 ECO dynamite. Four different experiments were designed in order to explain the presence of methenamine detected in the dynamite samples analyzed. In the first one, GC-MS was used to analyze the individual components of Goma-2 ECO provided by the manufacturer and the components mixed in a raw paste. Methenamine was detected in the manufacturer's ammonium nitrate and in the raw paste. The other experiments were designed to find the precursors sources for methenamine generation in Goma-2 ECO. Results revealed that these sources could be ammonium nitrate for ammonia and sawdust for formaldehyde. Under heating conditions, dynamite could produce these precursors, which could condense in the injection port of the GC-MS system and generate methenamine. However, methenamine was not always detected in these dynamites. This was explained by the existence of two opposite effects: (a) dynamite stability makes difficult that ammonium nitrate releases ammonia and (b) there is a gradual loss of formaldehyde in sawdust along the time. Both effects can prevent the formation of an amount of methenamine large enough to be detected.     

Identification process in mass graves from the Spanish Civil War II

The identification process of a mass grave from the Spanish Civil War (1936-1939) is presented. The presumed location of the grave, as well as the presumed number and identities of the persons buried in the grave were obtained exclusively from witnesses' and relatives' testimonies. In agreement with the testimonies, the grave was located at the indicated location and five skeletons were exhumed. Also in agreement with the testimonies, the osteological and DNA study led investigators to propose the identification of two kin groups, a father and his son and a pair of brothers. But the genetic study did not support the identification of a fifth man presumed to have been buried in the grave. The differences and similarities between this case and another case reported earlier are discussed.     

Permanent teeth development in a Spanish sample. Application to dental age estimation

The purpose of this study is to clarify the chronology of different stages of dental development, according to Demirjian, in a sample of Spanish children, which will enable us to build a database that will be used as a reference in regard to the dental development of individuals of our socio-geographic environment. In the same studied sample, a calculation of the dental age according to Demirjian was carried out. This study was conducted in a final sample consisting of 1010 orthopantograms, corresponding to Spanish children (485 boys and 525 girls) ages 2-16. Comparing the age of onset of the different stages among the children, evidence was found that girls had an earlier general development than boys. These differences were only statistically significant in teeth and concrete stages. The canine teeth revealed greater gender dimorphism, with significant differences in all stages compared with the upper canines. The method proposed by Demirjian for dental age calculation resulted in a significant overestimation of dental age in relation to the chronological age in boys (average of 0.87 years) and girls (average of 0.55 years). Data from this study may be used as reference for dental maturity, as well as a standard for estimating age in Spanish children.     

Self-protective behaviors and injury in domestic violence situations: does it hurt to fight back?

Research examining the impact of self-protective behaviors on outcomes in nonsexual assaults involving intimates has focused solely on one mainstream sample (National Crime Victimization Survey) in which violence is a relatively rare event. Using the Women's Experience of Violence (WEV) project which collects data from a sample of incarcerated women, we explored the phenomenon of self-protective behaviors to assess whether their use impacts the probability and severity of subsequent injury during a domestic violence incident. In addition to utilizing a unique sample, we considered an alternative operationalization of self-protective behaviors that separates physical and verbal responses to include whether the behavior involved an element of force. Results suggest some similarities between a mainstream sample and our marginalized sample. Namely, the frequency in which they utilize countermeasures and the effect of "fighting back" appear to be consistent with previous research. However, the more nuanced categorization of self-protective behaviors demonstrates the importance of considering whether the strategy was forceful when examining women's responses to violence.     

Predicting discharge in forensic psychiatry: the legal and psychosocial factors associated with long and short stays in forensic psychiatric hospitals

In Germany, both the number of patients treated in forensic psychiatric hospitals and the average inpatient treatment period have been increasing for over thirty years. Biographical and clinical factors, e.g., the number of prior offences, type of offence, and psychiatric diagnosis, count among the factors that influence the treatment duration and the likelihood of discharge. The aims of the current study were threefold: (1) to provide an estimate of the German forensic psychiatric patient population with a low likelihood of discharge, (2) to replicate a set of personal variables that predict a relatively high, as opposed to a low, likelihood of discharge from forensic psychiatric hospitals, and (3) to describe a group of other factors that are likely to add to the existing body of knowledge. Based on a sample of 899 patients, we applied a battery of primarily biographical and other personal variables to two subgroups of patients. The first subgroup of patients had been treated in a forensic psychiatric hospital according to section 63 of the German legal code for at least ten years (long-stay patients, n=137), whereas the second subgroup had been released after a maximum treatment period of four years (short-stay patients, n=67). The resulting logistic regression model had a high goodness of fit, with more than 85% of the patients correctly classified into the groups. In accordance with earlier studies, we found a series of personal variables, including age at first admission and type of offence, to be predictive of a short or long-stay. Other findings, such as the high number of immigrants among the short-stay patients and the significance of a patient's work time before admission to a forensic psychiatric hospital, are more clearly represented than has been observed in previous research.     

The Influence of the Relationship and Motivation on Inequity Aversion in Dogs

One crucial element for the evolution of cooperation may be the sensitivity to others?? efforts and pay-offs in comparison with one??s own costs and gains. If an individual responds to a disadvantageous reward distribution, then it would likely increase its relative fitness compared with those who do not (Brosnan & de Waal, Nature 428:140, 2004). Recent experimental research indicates that sensitivity toward unequal reward distribution is not a uniquely human trait; non-human primates react to inequity if they witness a conspecific that obtains a more attractive reward for the same effort. However, primates are not the only species that cooperate and thus would benefit from inequity aversion. Canids also frequently cooperate with group members (e.g., wolves, wild dogs, etc.) or humans (domestic dogs), allowing for the possibility that they also are sensitive to the reward distribution. We report the findings of two studies of dogs?? responses to inequity and the social factors that mediate such responses. In the first study, we investigated whether domestic dogs showed a response to the inequity of rewards received for the same action in pairs of familiar dogs. We found that dogs showed significant behavioral differences when tested without food rewards when in the presence of a rewarded partner compared to the baseline and asocial control situations. This indicated that the presence of a rewarded partner matters (Range, Horn, Virányi, & Huber, Proc Natl Acad Sci USA 106(1):340?C345, 2009). In contrast to primates, dogs did not react to differences in the quality of food or effort. In the second study, we analyzed whether individual (motivational or personality) characteristics determined the response of each subject to unequal reward distributions or whether the subject??s responses depended on the specific relationship they had with its partner. We found that individual motivation and the quality of the relationship influenced the dogs?? performance in test conditions, but that these factors varied across the control and reward inequity conditions. Overall, our results suggest that inequity aversion in dogs is conditional on their and their partner??s rewards. Whether inequity aversion is based on the same mechanisms in both humans and non-human primates is still unclear.     

DEVELOPMENTAL PATTERNS OF ALCOHOL USE IN RELATION TO THE PERSISTENCE AND DESISTANCE OF SERIOUS VIOLENT OFFENDING AMONG AFRICAN AMERICAN AND CAUCASIAN YOUNG MEN*

This study examined the association of alcohol use with the persistence and desistance of serious violent offending among African American and Caucasian young men from adolescence into emerging adulthood. Five violence groups were defined: nonviolent, late‐onsetters, desisters, persisters, and one‐time offenders. We examined alcohol use trajectories for these groups spanning 12 through 24/25 years of age using a four‐piecewise linear growth model s 12–14, 14–18, 18–21, and 21–24/25 years of age. The persisters and desisters reported the highest levels of drinking at 13 years of age. From 14 to 18 years old, however, the late‐onsetters showed a higher rate of increase in drinking, compared with the persisters and desisters. Starting at 18 years of age, the desisters’ drinking trajectory started to resemble that of the nonviolent group, who showed the highest rate of increase in drinking during emerging adulthood. By 24/25 years of age, the persisters could not be distinguished from the late‐onsetters, but they were lower than the nonviolent and one‐timer groups in terms of their drinking. At 24/25 years old, the desisters were not significantly different from the other violence groups, although they seemed most similar to the nonviolent and one‐timer groups. We found no evidence that the association between drinking and violence differed for African Americans and Caucasians. The findings suggest that yearly changes in alcohol use could provide important clues for preventing violent offending.