Results of the 2003-2004 GEP-ISFG collaborative study on mitochondrial DNA: focus on the mtDNA profile of a mixed semen-saliva stain

We report here a review of the seventh mitochondrial DNA (mtDNA) exercise undertaken by the Spanish and Portuguese working group (GEP) of the International Society for Forensic Genetics (ISFG) corresponding to the period 2003-2004. Five reference bloodstains from five donors (M1-M5), a mixed stain of saliva and semen (M6), and a hair sample (M7) were submitted to each participating laboratory for nuclear DNA (nDNA; autosomal STR and Y-STR) and mtDNA analysis. Laboratories were asked to investigate the contributors of samples M6 and M7 among the reference donors (M1-M5). A total of 34 laboratories reported total or partial mtDNA sequence data from both, the reference bloodstains (M1-M5) and the hair sample (M7) concluding a match between mtDNA profiles of M5 and M7. Autosomal STR and Y-STR profiling was the preferred strategy to investigate the contributors of the semen/saliva mixture (M6). Nuclear DNA profiles were consistent with a mixture of saliva from the donor (female) of M4 and semen from donor M5, being the semen (XY) profile the dominant component of the mixture. Strikingly, and in contradiction to the nuclear DNA analysis, mtDNA sequencing results yield a more simple result: only the saliva contribution (M4) was detected, either after preferential lysis or after complete DNA digestion. Some labs provided with several explanations for this finding and carried out additional experiments to explain this apparent contradictory result. The results pointed to the existence of different relative amounts of nuclear and mtDNAs in saliva and semen. We conclude that this circumstance could strongly influence the interpretation of the mtDNA evidence in unbalanced mixtures and in consequence lead to false exclusions. During the GEP-ISFG annual conference a validation study was planned to progress in the interpretation of mtDNA from different mixtures.     

Radiographic evaluation of third molar development in Spanish children and young people

Third molar development was evaluated in 786 young people aged between 4 and 20 years, all patients at the School of Dentistry of the University of Granada. The development of third molars and of mandibular second premolars and second molars was determined according to the stages proposed by Nolla. The onset of mandibular third molar formation was observed at very variable ages in this series, ranging from 5.86 to 14.66 years. The first developmental stages of maxillary third molars are not usually visible radiographically, which can lead to an incorrect diagnosis of agenesis. We found no significant relationship between the gender or age of the patient and the presence or absence of third molars. However, the presence/absence of the third molar can be predicted in 82.82% of cases when there is some degree of calcification of mandibular second molars and second premolars.     

DNA mixtures in forensic casework: a 4-year retrospective study

Occasionally interpretation guidelines from validation studies are difficult to apply to real forensic casework, especially in the case of mixed samples. Exogenous contamination, an unknown number of contributors or unbalanced proportion of each one in the sample and a varied degree of degradation of the biological materials, contribute to the difficulties in the interpretation of sample profiles. In this paper we have reviewed all the mixed genetic STR profiles encountered in our laboratory over 4 years (1997-2000) and evaluated the problems in the interpretation of the results. From 1547 criminal cases with 2424 samples typed, 163 showed a mixed profile (6.7%). We have observed that occasionally, a mixture appeared in the same sample with one multiplex amplification kit (e.g. Blue) and not with another (e.g. Green). From our results, it can be suggested that technical characteristics of the different fluorochrome groups in the multiplexes override the molecular characteristics of each STR in their capacity to detect mixtures.     

Globalization and Migration: The Impact of Family Remittances in Latin America

Taking as its point of departure the relationship between migration and globalization, this article highlights the salience of remittances in the national economies of Latin America, especially Mexico, Central America, and the Caribbean. It looks at the various actors that participate in the transfer of remittances and suggests that incorporating migrant labor dynamics as a category of economic integration will reveal a distinct landscape in the economies of Latin America.     

Book Reviews

Guy Edwards and J. Timmons Roberts, A Fragmented Continent: Latin America and the Global Politics of Climate Change. Cambridge: MIT Press, 2015. Table, figure, notes, bibliography, index, 274 pp.; hardcover $55, paperback $30, ebook $21. Owen Logan, John‐Andrew McNeish, and Axel Borchgrevink, eds., Contested Powers: The Politics of Energy and Development in Latin America. London: Zed Books, 2015. Tables, figures, bibliographies, index, 366 pp.; hardcover $95, paperback $28.95, ebook. Barbara Fritz and Lena Lavinas, eds., A Moment of Equality for Latin America? Challenges for Redistribution. Burlington: Ashgate, 2015. Tables, figures, bibliography, index, 284 pp.: hardcover $95.96, ebook. Mala Htun, Inclusion Without Representation in Latin America: Gender Quotas and Ethnic Reservations. New York: Cambridge University Press, 2016. Tables, figures, acronyms, appendixes, bibliography, index, 226 pp.; paperback $29.99. Koonings, Kees, and Dirk Kruijt, eds., Violence and Resilience in Latin American Cities. London: Zed Books, 2015. Figures, tables, 208 pp.; paperback $29.95, ebook. Miguel Carter, ed., Challenging Social Inequality: The Landless Rural Workers Movement and Agrarian Reform in Brazil. Durham: Duke University Press, 2015. Photographs, maps, tables, figures, abbreviations, bibliography, index, 544 pp.; hardcover $109.95, paperback $31.95. J. Patrice McSherry, Chilean New Song: The Political Power of Music, 1960s–1973. Philadelphia: Temple University Press. 2015. Notes, index, 229 pp.; hardcover $97.50, paperback $44.95, ebook $44.95. Bruce M. Bagley and Jonathan D. Rosen, eds., Colombia's Political Economy at the Outset of the Twenty‐First Century: From Uribe to Santos and Beyond. New York: Lexington Books, 2015. Figures, tables, notes, bibliography, index, 354 pp.; hardcover $100, ebook $99.99. Amy Reynolds, Free Trade and Faithful Globalization: Saving the Market. New York: Cambridge University Press, 2014. Tables, abbreviations, bibliography, index, 198 pp.; hardcover $93, ebook $72.     

UNFCCC negotiations (pre-Kyoto to COP-9): what the process says about the politics of CDM-sinks

One of the most contentious issues in the negotiations aimed at operationalizing the Kyoto Protocol was the treatment of sinks and, particularly, the eligibility of sinks projects in the Clean Development Mechanism (CDM). This paper attempts to analyse the politics underlying these negotiations, drawing on methods of process tracing, key informant interviews, negotiating texts and secondary literature. Tracing the sinks debate and highlighting key lessons about the nature of global environmental agreements and their institutional arrangements is the first step to recounting the history of the politics of one of the major contemporary international environmental debates. The paper shows that the Kyoto Protocol negotiations on sinks and CDM-sinks were multilaterally supported as a practical solution, but went ‘off track’ due to actors’ interests and tradeoffs. As regards future negotiations on forest sinks in developing countries under the framework of the UNFCCC, the paper argues that these are likely to be influenced by similar constraints, and also by the conservation and development agenda of its supporters; as well as the experience gathered on the CDM and the interests and concerns of developing countries. We broadly frame the paper within the literature on global environmental politics.

Bausteine einer Theorie der Netzwerkgesellschaft

Dieser Aufsatz präsentiert Bausteine einer Theorie der Netzwerkgesellschaft. Sie ist das Ergebnis empirischer und kulturvergleichender Untersuchungen und beschreibt in einer ersten Annäherung die das Informationszeitalter charakterisierende Gesellschaftsstruktur. In einer Vielzahl kultureller und institutioneller Erscheinungsweisen prägt die Netzwerkgesellschaft die meisten Gesellschaften dieser Erde auf ähnliche Weise wie die Industriegesellschaft die Gesellschaftsstruktur des Kapitalismus und der Planwirtschaft über weite Strecken des 20. Jahrhunderts geprägt hat. Gesellschaftsstrukturen organisieren sich um Verhältnisse von Produktion und Konsum, Macht und sozialer Erfahrung. Deren jeweilige raum-zeitliche Konfigurationen begründen Kulturen, die von sozialen Akteuren geschaffen, von ihnen reproduziert und durch sie transformiert werden. Ein zentrales Charakteristikum der Gesellschaftsstruktur im Informationszeitalter ist, dass sie auf Netzwerken beruht, die die soziale Morphologie kennzeichnen. Im Gegensatz zu früheren Formen stützen sich Netzwerke heute auf die neuen Informations- und Kommunikationstechnologien, so dass sie sowohl den Erfordernissen flexibler Dezentralisierung als auch jenen effektiver Entscheidungsfindung gerecht werden können. Dieser Aufsatz untersucht die spezifische Interaktion zwischen der Netzwerkmorphologie und den Verhältnissen von Produktion und Konsum, Macht und sozialer Erfahrung sowie der Kultur im Prozess einer neu entstehenden Gesellschaftsstruktur zu Beginn des 21. Jahrhunderts.