Development of a real-time PCR assay to control the illegal trade of meat from protected capercaillie species (Tetrao urogallus)

A rapid and highly species-specific real-time polymerase chain reaction (PCR) assay has been developed for the detection of capercaillie DNA (Tetrao urogallus) in meat and meat mixtures. The method combines the use of capercaillie-specific primers, that amplify a 142bp fragment of the mitochondrial 12S rRNA gene, and a positive control primer pair that amplifies a 141bp fragment of the nuclear 18S rRNA gene from eukaryotic DNA. SYBR(?) Green dye or TaqMan(?) fluorogenic probes were used to monitor the amplification of the target genes. Results obtained with the use of TaqMan(?) probes as detection platform increased the specificity of the real-time PCR assay in comparison with the results obtained using SYBR(?) Green. The proposed real-time PCR assay represents a rapid and straightforward method for the accurate identification of capercaillie that could be used by law enforcement agencies as a tool for the control of poaching and illegal trade of meat from this protected species.     

Methadone determination in puparia and its effect on the development of Lucilia sericata (Diptera, Calliphoridae)

This paper describes a sensitive UPLC-MS/MS method for quantification of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in single empty puparial case of Lucilia sericata. Larvae were reared on substrates spiked with different concentrations of methadone (0-4 μg/g). Methadone was quantified in puparia reared on high concentrated substrates (0.8-4 μg/g). The major metabolite of methadone (EDDP) was not detected, confirming rapid elimination of metabolites by the larvae before pupation. The effects of methadone on the development of L. sericata were also investigated. No effect on sex ratio was detected. A significant difference was calculated for emerged adults but no trends could be observed. Concerning the developmental curve, a significant difference was observed between control and high methadone concentrations using the Kolmogorov-Smirnov test.     

Population data for 16 Y-chromosome STRs in four populations from Pyrenees (Spain)

Population frequencies for the eight Y-STR loci included in the "minimal haplotype" from Y-STR Haplotype Reference Database (YHRD) plus other eight Y-STRs (DYS434, DYS435, DYS436, DYS437, DYS438, DYS439, GATA H4 and GATA A10) were obtained for a sample of 133 males from four main geographical areas in the Pyrenees (Spain): Vall D'Aran (Lérida), Cerdanya (Gerona), Alt Urgell (Lérida) and Jacetania (Huesca). Haplotype diversities were estimated in the four populations.     

Soil Fungi: Their Potential use as a Forensic Tool

As a grave is an anomalous environment and differs from its surroundings, criminal investigators employ different techniques for locating, recovering, and analyzing clandestine graves. In this study were identified the fungi found in the soil under corpses in decomposition with an aim at relating the copresence of human remains and different fungal species. Were isolated the fungi in three ways: soil washing, serial dilutions, and moist chamber growth. Dichotomomyces cejpii, Talaromyces trachyspermus, Talaromyces flavus, and Talaromyces sp. were the representative species found—with those belonging to the ammonia group, whose fungi are the first in the succession of cadaver decomposition directly in the ground. The mycobiota found at the present study area clearly differs to mycobiota identified in control sample and from previously described species for other areas of Buenos Aires Province, Argentina. Further forensic examples of this type are needed to develop fully the detailed use of mycology as a forensic tool.     

Early Repolarization,Acute Emotional Stress and Sudden Death

We herein report the case of a 36‐year‐old man who died suddenly after a fight with another man. Forensic investigations included unenhanced computed tomography, postmortem angiography, autopsy, histology, neuropathology, toxicology, and biochemistry and allowed a traumatic cause of death to be excluded. An electrocardiogram recorded some years prior to death revealed the presence of an early repolarization pattern. Based on the results of all investigations, the cause of death was determined to be cardiac arrhythmia and cardiac arrest during an emotionally stressful event associated with physical assault. Direct third party involvement, however, was excluded, and the manner of death was listed as natural. The case was not pursued any further by the public prosecutor.     

Are bar associations anticompetitive? An empirical analysis of recommended prices for legal services in Spain

The European Commission Report on Competition in Professional Services found that recommended prices by professional bodies have a significant negative effect on competition since they may facilitate the coordination of prices between service providers and/or mislead consumers about reasonable price levels. Professional associations argue, first, that a fee schedule may help their members to properly calculate the cost of services avoiding excessive charges and reducing consumers’ searching costs and, second, that recommended prices are very useful for cost appraisal if a litigant is condemned to pay the legal expenses of the opposing party. Thus, recommended fee schedules could be justified to some extent if they represented the cost of providing the services. We test this hypothesis using cross-section data on a subset of recommended prices by 83 Spanish bar associations and cost data on their territorial jurisdictions. Our empirical results indicate that prices recommended by bar associations are unrelated to the cost of legal services. Therefore, we conclude that fee schedules are not playing the role of providing useful cost information to practitioners and therefore this efficiency justification is weak.     

Participatory communication for development in practice: the case of community media

Since it emerged, the field of communication for development has undergone a constant process of redefinition. Since the 1990s, the importance of participation in social and communicative processes has been stressed, and studies carried out during those years focused on participation as an important component to be considered. The so-called community media are privileged forces driving the participatory communication for development approach. Since their emergence and up to the recent studies, community media have been characterised by the centrality of citizenship participation in the creation of widespread messages, and in the processes of social change that they promote.

Communication participative pour le développement dans la pratique : le cas des médias communautaires

Depuis son apparition, le domaine de la communication pour le développement a traversé un processus constant de redéfinition. Depuis les années 1990, l'importance de la participation aux processus sociaux et de communication a été soulignée, et des études effectuées durant cette période-là se sont concentrées sur la participation comme élément important à prendre en compte. Ces « médias communautaires » sont des forces privilégiées qui impulsent l'approche participative pour la communication pour le développement. Depuis leur naissance et jusqu'aux récentes études effectuées, les médias communautaires se sont caractérisés par la position centrale de la participation citoyenne à la création de messages généralisés, et aux processus de changement social qu'ils promeuvent.

Comunicación participativa para el desarrollo en la práctica: el caso de los medios comunitarios

Desde que inició, el ámbito de la comunicación para el desarrollo ha experimentado un proceso constante de redefinición. A partir de los años noventa, ha comenzado a insistirse en la importancia de participar en los procesos sociales y comunicacionales. Los estudios realizados durante esa década, se centraron en la participación como componente primordial que debía ser tenido en cuenta. Los llamados medios comunitarios constituyen fuerzas privilegiadas que impulsan el enfoque de la comunicación participativa para el desarrollo. Desde que iniciaron sus actividades y hasta las últimas investigaciones realizadas al respecto, los medios comunitarios se caracterizaron por la significación otorgada a la participación ciudadana en la creación de mensajes de amplia difusión y en el proceso de cambio social promovido por dichos mensajes.

Comunicação participativa para o desenvolvimento na prática: o caso da mídia comunitária

Desde que surgiu, a área de comunicação para o desenvolvimento tem passado por um processo constante de redefinição. Desde a década de 1990, a importância da participação em processos sociais e comunicativos tem sido ressaltada e estudos têm sido realizados durante estes anos tendo como foco a participação como componente importante a ser considerado. A chamada mídia comunitária constitui-se em forças privilegiadas que dirigem a comunicação participativa para a abordagem de desenvolvimento. Desde o seu surgimento até estudos recentes, a mídia comunitária tem se caracterizado pela ênfase na participação dos cidadãos na criação de mensagens disseminadas e nos processos de mudança social que elas promovem.     

Identification of Human Remains by DNA Analysis of the Gastrointestinal Contents of Fly Larvae

Dipterous fly larvae (maggots) are frequently collected from a corpse during a criminal investigation. Previous studies showed that DNA analysis of the gastrointestinal contents of maggots might be used to reveal the identity of a victim. However, this approach has not been used to date in legal investigations, and thus its practical usefulness is unknown. A badly burned body was discovered with its face and neck colonized by fly larvae. Given the condition of the body, identification was not possible. Short tandem repeat (STR) typing was performed using the gastrointestinal contents of maggots collected from the victim and was compared to STR profiles obtained from the alleged father. The probability of paternity was 99.685%. Thus, this comparative DNA test enabled the conclusive identification of the remains. This is the first reported case of analysis of human DNA isolated from the gastrointestinal tract of maggots used to identify a victim in a criminal case.     

STR data for nine Y-chromosomal loci in Guinea Equatorial (central Africa)

Allele and haplotype frequencies for nine Y-specific STR loci (DYS19, DYS389-I, DYS389-II, DYS390, DYS391, DYS393, DYS434, DYS437, and DYS439) were obtained from a sample of 57 males from Guinea Equatorial.