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201.
SNPs in forensic genetics: a review on SNP typing methodologies 总被引:17,自引:0,他引:17
There is an increasing interest in single nucleotide polymorphism (SNP) typing in the forensic field, not only for the usefulness of SNPs for defining Y chromosome or mtDNA haplogroups or for analyzing the geographical origin of samples, but also for the potential applications of autosomal SNPs. The interest of forensic researchers in autosomal SNPs has been attracted due to the potential advantages in paternity testing because of the low mutation rates and specially in the analysis of degraded samples by use of short amplicons. New SNP genotyping methods, chemistries and platforms are continuously being developed and it is often difficult to be keeping up to date and to decide on the best technology options available. This review offers to the reader a state of the art of SNP genotyping technologies with the advantages and disadvantages of the different chemistries and platforms for different forensic requirements. 相似文献
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Suraje?DessaiEmail author E.?Lisa?F.?Schipper Esteve?Corbera Bo?Kjellén María?Gutiérrez Alex?Haxeltine 《International Environmental Agreements: Politics, Law and Economics》2005,5(2):105-124
From 1 to 12 December 2003, the Ninth Session of the Conference of the Parties to the United Nations Framework Convention took place in Milan, Italy. This conference continued the laborious effort of developing an international climate regime by preparing for the Kyoto Protocol’s entry into force. Some two dozen decisions were adopted on a wide range of options for responding to climate change. This paper assesses the progress achieved at the conference on a number of issues. Among these were operational details for implementing forestry projects under the Convention’s Clean Development Mechanism, and guidelines for reporting on greenhouse gas emissions and removals from agriculture, forestry and land-use change. Parties also decided on rules with respect to two funds, the Special Climate Change Fund and the Least Developed Country Fund. With respect to developing countries, Parties continued discussions on rules for building response capacity in light of the expected adverse effects of climate change and transferring environmentally sound technology. They also discussed how to incorporate scientific advice from the Third Assessment Report of the Intergovernmental Panel on Climate Change into the negotiations. Although Russia did not ratify the Kyoto Protocol prior to the conference, Milan demonstrated momentum and interest among Parties to support the climate regime. Nevertheless, it is doubtful whether the detailed discussions were able to contribute to preparing for the long term. To this end, this paper concludes that more discussion and leadership is required to bridge the North/South gap if a post-2012 climate regime is to stand. 相似文献
204.
Gamero JJ Romero JL González JL Carvalho M Anjos MJ Real FC Vide MC 《Forensic science international》2002,125(1):86-89
The Y-chromosome polymorphism of eight STRs (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392; DYS393, DYS385) were studied in 111 unrelated individuals from the population of southwest Spain. The most common haplotype was shared by 3.6% of the sample, while 99 haplotypes were unique. The gene diversity was 0.9977. 相似文献
205.
Parreira KS Lareu MV Sánchez-Diz P Skitsa I Carracedo A 《Forensic science international》2002,126(3):261-264
Haplotype, allele frequencies and population data of nine Y-chromosome STR loci, DYS385I, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, were determined from a sample of 69 unrelated Greek male individuals. 相似文献
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Qualitative data from a case study of the Jamaica social investment fund reveal that the social fund process is elite-driven and decision-making tends to be dominated by a small group of motivated individuals. However, there is broad-based satisfaction with the outcome. Quantitative data from 500 households mirror these findings by showing that, ex-ante, the social fund does not address the expressed needs of the majority of individuals in the majority of communities. By the completion of the project, however, 80 per cent of the community expresses satisfaction with the outcome. An analysis of the determinants of participation reveals that better educated and better networked individuals dominate the process. Propensity-score analysis demonstrates that JSIF has had a causal impact on improvements in trust and the capacity for collective action, but these gains are greater for elites. 相似文献
209.
José María Rosales 《Journal of Political Ideologies》2016,21(3):225-241
This article explores the practices of democracy in Spain through the lens of its 20th-century constitutional moments, namely, those around the 1931 and the 1978 constitutions, with the aim of elucidating its changing ideological significance. Whereas in 1931, supporting democracy had a strongly partisan meaning, as every endorsement of the democratic ideal entailed a conflicting understanding of the Republic’s democratic character, in 1978 it gave rise to an integrative form of pluralism. Even if the former has left a deep imprint on Spanish politics, anticipating a kind of seemingly irreconcilable opposition between left and right, the latter has proved instrumental in the consolidation of the new democratic regime. After the institutional distortion of democracy during the four-decade dictatorship’s ‘organic democracy’, since 1977 the ideal of democracy was thoroughly recast into a pluralist ideology. Unlike views describing it as a consensual regime, the article argues that the politics of consensus tested since the 1977 constitutional debates paved the way for the accommodation of democratic pluralism; furthermore, the culture of pacts it contributed to set up has continued to inspire a new form of adversarial, parliamentary politics well beyond the transition years. 相似文献
210.
Improved estimation of postmortem interval based on differential behaviour of vitreous potassium and hypoxantine in death by hanging 总被引:7,自引:0,他引:7
Muñoz Barús JI Suárez-Peñaranda J Otero XL Rodríguez-Calvo MS Costas E Miguéns X Concheiro L 《Forensic science international》2002,125(1):67-74
Many formulae are available to estimate the relation between the potassium ([K+]) and hypoxantine ([Hx]) concentration in the vitreous humour and the postmortem interval (PMI). Typically these have been based on a correlation test and linear regression using the postmortal interval as the independent variable and [K+] or [Hx] as the dependent variable in order to estimate the confidence interval. However, a recent study has shown that a more precise measurement of PMI can be obtained if [K+] is used as the independent variable. The regression lines obtained from the most recent deceased subjects with forensic relevance received for autopsy in the Institute of Legal Medicine are [K+] = 5.589 + 0.174PMI and [Hx] = 26.459 + 3.017PMI, by changing the variables, we obtain PMI=3.967[K+] - 19.186 (R2 = 0.688, P < 0.001) and PMI = 0.172 [Hx] + 0.170 (R2 = 0.518, P < 0.001). In this paper we propose the cause of death as an extra factor which modifies the relationship and gives even greater precision in estimating PMI. In cases of death by hanging the results are considerably improved with [K+] = 5.224 + 0.225PMI and [Hx] = 15.161+4.957PMI, respectively, and consequently, PMI = 3.631[K+] - 17.334 (R2 = 0.818, P< 0.001) and PMI = 0.153[Hx] - 0.368 (R2 = 0.757, P < 0.001): the slope is less and the precision is obviously enhanced. 相似文献