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341.
342.
Andrew J. Schweighardt Ph.D. Amanda Battaglia M.S. Margaret M. Wallace Ph.D. 《Journal of forensic sciences》2014,59(1):15-33
A bead‐based liquid hybridization assay, Luminex® 100?, was used to identify four pathogenic bacteria, Bacillus anthracis, Clostridium botulinum, Francisella tularensis subsp. tularensis, and Yersinia pestis, and several close relatives. Hybridization between PCR‐amplified target sequences and probe sequences (located within the 23S ribosomal RNA gene rrl and the genes related to the toxicity of each bacterium) was detected in single‐probe or multiple‐probe assays, depending on the organism. The lower limits of detection (LLDs) for the probes ranged from 0.1 to 10 ng. Sensitivity was improved using lambda exonuclease to digest the noncomplementary target strand. All contributors in 33 binary, ternary, and quaternary mixtures in which all components were present in a 1:1 ratio were identified with an 80% success rate. Twenty‐eight binary mixtures in which the two components were combined in various ratios were further studied. All target sequences were detected, even when the minor component was overshadowed by a tenfold excess of the major component. 相似文献
343.
Margaret J. Wyszomirski 《国际公共行政管理杂志》2013,36(1-2):45-81
Public administration is no longer simply concerned with the management of governmental organizations. Rather it includes a range of proxy and third-party organizations of both a for-profit and non-profit character. This blurring of public-private boundaries is further compounded by the recognition of a third and non-profit sector engaged in societal purposes and composed of a diversity of actuative organizations. Conceptual and definitional clarification is required to bring the blurred boundaries between the three sectors -- and especially the third sector itself -- into better analytical focus. Using a set of attributes common to all organizations, this article develops a comparative typology of organizations across the three sectors as well as identifies subtypes within each sector. By articulating the structural differences and similarities among organizations, analysis can proceed as to how cooperation and collaboration in addressing the public's business can be better managed. 相似文献
344.
Joli Bregu B.S. Danielle Conklin M.S. Elisse Coronado M.S. Margaret Terrill M.S.F.S. Robin W. Cotton Ph.D. Catherine M. Grgicak Ph.D. 《Journal of forensic sciences》2013,58(1):120-129
Determining appropriate analytical thresholds (ATs) for forensic DNA analysis is critical to maximize allele detection. In this study, six methods to determine ATs for forensic DNA purposes were examined and compared. Four of the methods rely on analysis of the baseline noise of a number of negatives, while two utilize the relationship between relative fluorescence unit signal and DNA input in the polymerase chain reaction (PCR) derived from a dilution series ranging from 1 to 0.06 ng. Results showed that when a substantial mass of DNA (i.e., >1 ng) was amplified, the baseline noise increased, suggesting the application of an AT derived from negatives should only be applied to samples with low levels of DNA. Further, the number and intensity of these noise peaks increased with increasing injection times, indicating that to maximize the ability to detect alleles, ATs should be validated for each post‐PCR procedure employed. 相似文献
345.
Margaret Stephenson 《Commonwealth Law Bulletin》2013,39(3):545-570
In Australia, land rights legislation provides statutory schemes for the transfer of land to Indigenous peoples. The first significant land rights legislation was passed by the Australian Commonwealth government in 1976. This was the Aboriginal Land Rights (Northern Territory) Act (ALRA) 1976 (Cth). In 2006, the Australian Commonwealth government passed significant amendments to the ALRA. One of the key amendments introduced the leasing of Aboriginal ‘township lands’ held under that Act. It is these leasing amendments which are a focus of this article. A primary motivation behind the amendments was to decrease poverty in Indigenous communities and to allow for economic development on Aboriginal lands. This article critiques the township leasing scheme under the amended Act. It questions whether the new leasing arrangements are the most appropriate forms of leasing to achieve economic development on Aboriginal lands and to benefit the Aboriginal communities who hold these lands. In 2008, leasing amendments were passed to Queensland's statutory land scheme in the Aboriginal Land Act 1991 (Qld) and these amendments are subject to review in this article. Furthermore, the article examines alternative forms of leasing used for economic development on Indigenous reserve lands in Canada and whether there are lessons that Australia could learn from these tenures and their modes of leasing. 相似文献
346.
Dating violence among college aged couples has become a growing concern with increasing prevalence. The current study investigated the interplay among witnessing violence during childhood (both parental conflict and parent to child aggression), attachment insecurity, egalitarian attitude within the relationship, and dating aggression. Participants of this study included 87 couples. Results from the structural equation model indicated that the proposed model provided a good fit to the with a χ2 to df ratio of 1.84. In particular, both female and male participants who reported higher levels of attachment insecurity were more likely to be victim of dating aggression in their relationships. Furthermore, female participants who reported having witnessed parental conflict were more likely to be victimized by their partners. In conclusion, this study provides a comprehensive understanding of intimate relationship violence with dyadic data showing, for both genders, attachment insecurity is a crucial factor in both victimization and perpetration of aggression. 相似文献
347.
Although the long-term effects of cumulative childhood maltreatment (CCM) include a variety of adverse consequences, many individuals are resilient following such experiences. This study explored the role of social support from family and friends in buffering long-term outcome following CCM, examining both main and moderating effects. Participants included 344 college aged men and women. Findings revealed strong promotive (main) effects of social support from family and friends that were associated with a reduction in symptoms of depression/anxiety and anger/hostility, regardless of the severity of abuse experienced. Support generally acted as a buffer (moderator) against negative outcomes for individuals with lower, and not higher, levels of CCM. The role of family support in moderating outcome was complex, particularly with respect to women’s later dating victimization; here family support functioned as a protective factor at low CCM, but as a vulnerability factor at high CCM. 相似文献
348.
Henrik Andershed Margaret Kerr Håkan Stattin 《Journal of Scandinavian Studies in Criminology & Crime Prevention》2013,14(1):31-49
Two ideas concerning the link between bullying in school and violence on the streets are investigated. (1) Bullying and victimization in school is a product of the school situation and people's inability to choose their levels of exposure to others. According to this hypothesis, bullying is largely a phenomenon that is isolated to the school context. (II) Bullying behaviour in school and inflicting damage to others outside school is a reflection of a more general aggressive behaviour pattern and, hence, bullying in school and violence on the streets will, to a great extent, involve the same individuals. The literature offers suggestions that either could be the case. Participants were 2915 14-year-olds in a medium-sized county in Sweden who responded to a self-report questionnaire.Theresults showed that bullying others in school was strongly linked to violent behaviour and weapon-carrying on the streets, both among boys and girls. It was also found that bullying others in school was related to being violently victimized on the streets. The findings remained the same when statistically controlling for loitering and nights spent away from home, which were both related to bullying behaviour. It is concluded that bullying behaviour in school is in many cases a part of a more general violent and aggressive behaviour pattern and that preventive efforts targeting individuals with bullying behaviour in school could, according to the present study, decrease violence among adolescents out in the community as well. 相似文献
349.
Finding transformative fair use, the Second Circuit allowedpublisher Dorling Kindersley to reproduce in a book thumbnail-sizedversions of concert posters owned by the Bill Graham Archives. 相似文献
350.
Ponitz JE Olson BD Jason LA Davis MI Ferrari JR 《Journal of prevention & intervention in the community》2006,31(1-2):95-110
The current study examined medical care need and utilization patterns among a substance abusing and recovering population (n = 876), investigating factors such as employment and drug use categories (e.g., pharmaceutical use, cocaine use, heroin use, alcohol use). It was found that those who were unemployed needed and utilized greater medical care than those who were employed. Results indicated that heroin, cocaine, and/or alcohol use was not predictive of medical care need or utilization, whereas pharmaceutical drug use was predictive of medical care need and utilization. Trauma and risky use of substances were not significant predictors of medical care need or utilization whereas the suicide severity composite was significant. Potential implications for misuse of medical services (e.g., to obtain pharmaceutical drugs of use) and federal medical care expenditure allocation are discussed. 相似文献