Development of a Nomenclature System for a Canine STR Multiplex Reagent Kit*†

Abstract: Despite the popularity of dogs in US households, canine DNA evidence remains largely untapped in forensic investigations partially because of the absence of well‐defined forensic short tandem repeats (STRs), lack of standardized and validated PCR protocols, STR reagent kits, and poorly developed nomenclature. A nomenclature system was established based on internationally recognized recommendations for human forensic STRs for a recently developed canine STR reagent kit. Representative alleles were sequenced from each of the 18 STRs and the sex‐typing marker included in the kit. This study also reflects on the impact of point mutations, insertions, and deletions within and outside the STR core repeat structures. An understanding of the STRs’ sequence and repeat structures will enable development of a robust and reliable allele nomenclature and improve the accuracy and precision of allele fragment sizing in canine forensic profiling. The expected allele sizes have been calculated, and their repeat stuctures defined based on sequence information.     

The Effect of Decalcifying Solutions on Hemosiderin Staining

Abstract: To determine whether routine decalcification may reduce the amount of stainable iron that is visible on tissue sections, samples of liver and lung tissue with excessive iron stores were placed in three standard decalcifying solutions (i) formic acid [33%], formaldehyde [4%], and NaCl [0.85%]; (ii) formic acid [30%], formaldehyde [4%], and water; and (iii) nitric acid [5%] for 24, 48, 72, and 96 h. After exposure to the decalcifying solutions, the tissues were stained with Perls stain. The slides were examined blind and the intensity of iron staining was scored semiquantitatively from 0 to 3+. The trend in all samples over the course of the experiment (96 h) was for reduction in the intensity of hemosiderin staining. As the amount of stainable hemosiderin in tissues may be significantly altered by decalcification, the absence of hemosiderin in tissues adjacent to a fracture site does not necessarily indicate that the injury was acute.     

The Need for a Positive Psychological Approach and Collaborative Effort for Improving Practice in the Interrogation Room

The White Paper suggests important reforms that will reduce the likelihood of false confessions resulting from police interrogation. The research underlying these suggested reforms has yielded significant advances in our understanding of factors associated with false confessions. As we move forward, we encourage the development of empirically based approaches that provide a viable alternative to current practice. In doing so, we suggest that researchers pursue a positive psychological approach that involves partnering with practitioners to systematically develop interrogative methods that are shown to be more diagnostic. By taking such an approach, we believe that the recommendations offered in the current White Paper can be supplemented by methods that carry the support of both scientific and law enforcement communities.     

Crime trends through two decades of social changes in Spain

Over the last two decades, the Spanish social fabric has undergone a variety of profound changes, which in turn may explain the rise in police-recorded crime and prison population figures. Although the rise in crime reported to the police has been higher for common offences—misdemeanors—, the mainstream media concentrates overwhelmingly in serious violent crimes—felonies—. Spain does not have an official agency responsible for conducting victimization surveys to measure crime trends as directly experienced by the citizens. Based on this methodology, our study shows that contrary to information gathered from police data, and despite social fabric changes, crime in Spain is decreasing. The present study also reveals the distorted public perception of this trend, which seems to respond to a repetitive coverage of serious crime by the mainstream media.     

Policy learning as complex contagion: how social networks shape organizational beliefs in forest-based climate change mitigation

Policy Sciences - Policy learning can alter the perceptions of both the seriousness and the causes of a policy problem, thus also altering the perceived need to do...     

MEDICALLY ASSISTED HUMAN REPRODUCTIVE TECHNOLOGIES (ART) AND HUMAN RIGHTS — THE EUROPEAN PERSPECTIVE

The present article examines how the progress of science, and in particular, medically assisted human reproductive technologies (ART) have provoked a revolution in the sphere of family relations, generating a series of ethical and legal conflicts. The article focuses on the European perspective, without ignoring the international sphere, given the globalization of the phenomenon. The emerging legal issues are analyzed through the filter of international human rights, not only an important aspect to take into consideration in the context of bioethics in general, but a “passage obligé” given that certain concepts find their explanation and coordinates in international human rights law. It is from this perspective that the relationship between ART and human rights is presented. The applicable international and European legal instruments and principles shall be mentioned, as well as a brief comparison of national legal frameworks in Europe. The emerging bioethical and legal issues are examined in correlation with the response of the European Court of Human Rights through its case law aimed at balancing conflicting rights when faced with issues pertaining to ART. Lastly, the article presents in more detail the particular legal issues under debate in France and Italy, two European countries with specific legislation in the field.     

Comparison Among Manual Facial Approximations Conducted by Two Methodological Approaches of Face Prediction

This study verified the difference between two methods of forensic facial approximation (FFA) regarding recognition and resemblance rates. Three‐dimensional models of skulls were obtained from computerized tomography (CT) scans of two subjects (targets). Two manual FFAs were performed for each target, by applying two different guidelines for the facial structures (what we called “American method” (AM) and “Combined method” (CM)). Unfamiliar assessors evaluated the sculptures by recognition and resemblance tests. The AM was that which allowed more correct responses of recognition and higher resemblance's scores for the male target (p < 0.001). Regarding guidelines for modeling characteristics of the face, the ones that are practical and easily performed for sculptures, such as the length of the anterior nasal spine multiplied by 3 for nose prediction, may offer better results in terms of resemblance.     

Too Many or Too Few? The Increase in Federal Ministerial Advisers 1972–1999

This paper examines recently published figures that show the increase in ministerial staff between 1972 and 1996 (Dunn 1997). A careful examination of the table in Dunn's book reveals it gives a misleading impression of the number of ministerial staff; the number of advisory staff to ministers; and the growth in advisory resources over the Hawke–Keating period. By re-analysing the figures the paper reveals the reality of the growth in advisory staff to ministers in 1972–1999. It provides an account of the number of staff providing policy and political advice to ministers in ministerial offices, rather than total staff numbers. It reveals advisory resources to ministers have grown significantly since the introduction of ministerial advisers in 1972. However, the growth in adviser numbers over the Hawke–Keating period was more modest than is suggested by Dunn's table. The paper provides the base data needed for a discussion of the increase in ministerial staff and whether there are too few or too many federal ministerial advisers.     

Duplications of the Y-chromosome specific loci P25 and 92R7 and forensic implications

In the present study, we demonstrate that two commonly used Y-chromosome single nucleotide polymorphisms (SNPs), P25 and 92R7, are paralogous sequence variants (PSVs) originating from segmental duplications and that at least one of the sequence variants in each group of loci is polymorphic. Several methodologies were used in order to detect the SNP alleles and the PSVs of the loci. All results obtained with the various typing techniques supported the conclusion. The allele distributions of the binary markers were analysed in more than 600 males with seven different haplogroups. For P25, the ancestral allele C was found in several samples from different haplogroups. The derived allele A was always present with an additional C variant. Haplogroup P was defined by the derived allele A at the 92R7 locus. However, the ancestral allele G was always associated with an A variant due to the duplication.