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排序方式: 共有427条查询结果,搜索用时 31 毫秒
151.
152.
Krenke BE Viculis L Richard ML Prinz M Milne SC Ladd C Gross AM Gornall T Frappier JR Eisenberg AJ Barna C Aranda XG Adamowicz MS Budowle B 《Forensic science international》2005,148(1):1-14
Y chromosome-specific short tandem repeat (Y-STR) analysis has become another widely accepted tool for human identification. The PowerPlex Y System is a fluorescent multiplex that includes the 12 loci: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. This panel of markers incorporates the 9-locus European minimal haplotype (EMH) loci recommended by the International Y-STR User Group and the 11-locus set recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Described here are inter-laboratory results from 17 developmental validation studies of the PowerPlex Y System and include the following results: (a) samples distributed between laboratories and commercial standards produced expected and reproducible haplotypes; (b) use of common amplification and detection instruments were successfully demonstrated; (c) full profiles were obtained with standard 30 and 32 cycle amplification protocols and cycle number (24-28 cycles) could be modified to match different substrates (such as direct amplification of FTA paper); (d) complete profiles were observed with reaction volumes from 6.25 to 50 microL; (e) minimal impact was observed with variation of enzyme concentration; (f) full haplotypes were observed with 0.5-2x primer concentrations; however, relative yield between loci varied with concentration; (g) reduction of magnesium to 1mM (1.5 mM standard) resulted in minimal amplification, while only partial loss of yield was observed with 1.25 mM magnesium; (h) decreasing the annealing temperature by 2-4 degrees C did not generate artifacts or locus dropout and most laboratories observed full amplification with the annealing temperature increased by 2 degrees C and significant locus dropout with a 4 degrees C increase in annealing temperature; (i) amplification of individual loci with primers used in the multiplex produced the same alleles as observed with the multiplex amplification; (j) all laboratories observed full amplification with >or = 125 pg of male template with partial and/or complete profiles observed using 30-62.5 pg of DNA; (k) analysis of < or = 500 ng of female DNA did not yield amplification products; (l) the minor male component of a male/female mixture was observed with < or =1200-fold excess female DNA with the majority of alleles still observed with 10,000-fold excess female; (m) male/male mixtures produced full profiles from the minor contributor with 10-20-fold excess of the major contributor; (n) average stutter for each locus; (o) precision of sizing were determined; (p) human-specificity studies displayed amplification products only with some primate samples; and (q) reanalysis of 102 non-probative casework samples from 65 cases produced results consistent with original findings and in some instances additional identification of a minor male contributor to a male/female mixture was obtained. In general, the PowerPlex Y System was shown to have the sensitivity, specificity and reliability required for forensic DNA analysis. 相似文献
153.
Validation of male-specific, 12-locus fluorescent short tandem repeat (STR) multiplex 总被引:1,自引:0,他引:1
Krenke BE Viculis L Richard ML Prinz M Milne SC Ladd C Gross AM Gornall T Frappier JR Eisenberg AJ Barna C Aranda XG Adamowicz MS Budowle B 《Forensic science international》2005,151(1):111-124
Y chromosome-specific short tandem repeat (Y-STR) analysis has become another widely accepted tool for human identification. The PowerPlex Y System is a fluorescent multiplex that includes the 12 loci: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. This panel of markers incorporates the 9-locus European minimal haplotype (EMH) loci recommended by the International Y-STR User Group and the 11-locus set recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Described here are inter-laboratory results from 17 developmental validation studies of the PowerPlex Y System and include the following results: (a) samples distributed between laboratories and commercial standards produced expected and reproducible haplotypes; (b) use of common amplification and detection instruments were successfully demonstrated; (c) full profiles were obtained with standard 30 and 32 cycle amplification protocols and cycle number (24-28 cycles) could be modified to match different substrates (such as direct amplification of FTA paper); (d) complete profiles were observed with reaction volumes from 6.25 to 50 microL; (e) minimal impact was observed with variation of enzyme concentration; (f) full haplotypes were observed with 0.5-2x primer concentrations; however, relative yield between loci varied with concentration; (g) reduction of magnesium to 1mM (1.5 mM standard) resulted in minimal amplification, while only partial loss of yield was observed with 1.25 mM magnesium; (h) decreasing the annealing temperature by 2-4 degrees C did not generate artifacts or locus dropout and most laboratories observed full amplification with the annealing temperature increased by 2 degrees C and significant locus dropout with a 4 degrees C increase in annealing temperature; (i) amplification of individual loci with primers used in the multiplex produced the same alleles as observed with the multiplex amplification; (j) all laboratories observed full amplification with >or = 125 pg of male template with partial and/or complete profiles observed using 30-62.5 pg of DNA; (k) analysis of < or = 500 ng of female DNA did not yield amplification products; (l) the minor male component of a male/female mixture was observed with < or =1200-fold excess female DNA with the majority of alleles still observed with 10,000-fold excess female; (m) male/male mixtures produced full profiles from the minor contributor with 10-20-fold excess of the major contributor; (n) average stutter for each locus; (o) precision of sizing were determined; (p) human-specificity studies displayed amplification products only with some primate samples; and (q) reanalysis of 102 non-probative casework samples from 65 cases produced results consistent with original findings and in some instances additional identification of a minor male contributor to a male/female mixture was obtained. In general, the PowerPlex Y System was shown to have the sensitivity, specificity and reliability required for forensic DNA analysis. 相似文献
154.
Gender Patterns of Research and Licensing Activity of Science and Engineering Faculty 总被引:1,自引:0,他引:1
Jerry?G.?ThursbyEmail author Marie?C.?Thursby 《The Journal of Technology Transfer》2005,30(4):343-353
In this paper we extend our earlier work on science and engineering faculty disclosure and licensing activity to examine a characteristic of faculty ignored in our earlier work – the gender of faculty in our database of over 4500 faculty at 11 major universities. Not surprisingly, women comprise only 8.55% of the faculty in our sample. They are most represented in the biological sciences and the women in the sample are more likely to be younger faculty. The most striking result from highlighting women in the sample is that women are less likely to disclose inventions than men despite the fact that there are no significant differences in publication patterns. While disclosure activity varies by gender, we find that the disclosure activity of women and men converges over the period of our sample.JEL Classification: J16, L3, O3Financial support was provided by National Science Foundation (SES 0094573), the Alan and Mildred Peterson Foundation and the Ewing Marion Kauffman Foundation. 相似文献
155.
156.
157.
158.
Valrie Martin Marie‐Hlne Jobin 《Canadian public administration. Administration publique du Canada》2004,47(3):304-331
Au cours des deux derniéres décennies, plusieurs gouvemements ont voulu réformer leur administration publique en mettant en place un cadre de gestion axée sur les résultats. Jusqu à tout récemment, la documentation scientifique n'abor‐dait que trés peu ce théme et, outre les travaux de l'OCDE, le concept de gestion axée sur les résultats et son contenu n' avaient pas été définis. Nous avons donc voulu éla‐borer une définition générique de la gestion axée sur les résultats et comprendre la façon dont elle s'intègre à différents aspects de la gestion des organisations. Pour ce faire, nous avons exploré le cadre de gestion proposé par les réformes mises de l'avant dans huit juridictions. Les pays et provinces canadiennes choisis ont une approche‐type de gestion anglo‐américaine, soit le Canada, le Québec, l'Ontario, l'Alberta, les États‐Unis, l'Australie, le Royaume‐Uni et la Nouvelle‐Zélande. Les modèles étudiés présentent plusieurs similarités. On observe que la gestion de la performance dans ces juridictions se déploie dans des cycles de gestion composés de plusieurs étapes. Les éléments communs de ces cycles sont la planification stratégique, la planification opérationnelle, la réalisation des plans et des programmes, la mesure ds résultats, la reddition de comptes et l'analyse et l'interpréta‐tion des résultats. Ces processus se concrétisent par la publication de documents. Par ailleurs, ces cadres de gestion ont tendance à s'intégrer aux autres enjeux de la gestion des organisations tels que la qualité du service à la clientèle, la gestion des res‐sources humaines et financières et la vérification. Abstract: Over the last two decades, several governments have wanted to reform their public administration by implementing a results‐based management framework. Until very recently, the scientific literature hardly addressed this issue and, other than the OECD work, the concept of results‐based management and its content had not been defined. We therefore wanted to develop a generic definition for results‐based management and to understand how it fits into various aspects of organizational management. To do so, we explored the management framework put forward through reforms introduced in eight jurisdictions. The countries and Canadian provinces chosen, i.e., Canada, Quebec, Ontario, Alberta, United States, Australia, United Kingdom and New‐Zealand, use the typical Anglo‐American management approach. The models considered have several similarities. We found that performance management in those jurisdictions was implemented throughout several steps of management cycles. Common elements in these cycles include strategic planning, operational planning, plan and program delivery, results measurement, accountability, and results analysis and interpretation. The process is documented through published documents. Moreover, these management frameworks tend to become part of the other organizational management components, such as quality of customer service, management of human and financial resources, and auditing. 相似文献
159.
This article, to be published in two parts, explores the debate between the Bertelsmann Foundation and the Centre for Democracy and Technology about regulation on illegal and harmful content on the Internet. The authors intend to analyze the method, interests and limits of the self regulatory model proposed by the European institutions, bearing in mind the users’ empowerment perspective. 相似文献
160.
In this article, we argue that when patron‐client relations are grounded in economic relationships, such as between landlord and worker, we should expect clientelism to influence not just how public policy, the state, and the political system work, but also how the economy works. We develop a simple model of the economic consequences of electoral clientelism when voting behavior can be observed. Landlords/patrons provide economic rents to workers, and in exchange workers vote for parties favored by landlords. As votes are used by the landlords to accumulate political rents, vote control increases the demand for labor and for land. The model implies that the introduction of the Australian ballot, which destroys this form of clientelism, should lead to a fall in the price of land in those areas where patron‐client relationships are strongest. We test the predictions of the model by examining in detail the evolution of land prices in Chile around May 31, 1958, for which we collected original data. A characteristic of rural Chile at this time were patron‐client relations based on the inquilinaje system, by which a worker, the inquilino, entered into a long‐term, often hereditary, employment relationship with a landlord and lived on his landlord’s estate. We show that the introduction of the Australian ballot in 1958 led to a fall of about 26% in land prices in the areas where these patron‐client relationships were predominant. 相似文献