A D19S433 primer binding site mutation and the frequency in Japanese of the silent allele it causes

Short tandem repeat studies are powerful tools for parentage analysis and for identification of missing persons, victims of murder, and victims of mass fatalities when reference samples are unavailable. The primer in the Identifiler kit failed to amplify an allele at the D19S433 locus, producing a silent ("null") allele. The causal mutation is a base change (G>A) 32 nucleotides downstream from the 3' end of the AAGG repeats. The silent alleles are problematical in parentage analysis because when transmitted, they can cause a parent-child inconsistency that is unrelated to Mendelian genetics. The inconsistency is sometimes termed an "apparent opposite homozygosity" and it produces false evidence of nonparentage. Alternative primers were designed to amplify the D19S433 locus alleles and they detect the silent allele. Frequencies of the (no longer) silent allele were determined to be 0.0114 in 176 people from Shizuoka (Honshu) and 0.0128 in 156 people from Okinawa.     

Analysis of prostitution in Japan

Before World War II, we witnessed the purification movement, on which Christian groups embarked as moral entrepreneurs. After the war, female groups took the initiative in a movement for anti‐prostitution law. The movement resulted in the enactment of the Prostitution Prevention Law in 1956, which made prostitution a punishable criminal offense. On the other hand, in the United States in the 1960s, some criminologists advocated the necessity of de‐criminalization of victimless crimes, one of which was prostitution. Some groups of feminists also insisted on the de‐criminalization of prostitution to emancipate women toward a freer and more liberated sexuality. New ideas regarding the control, or lack thereof, of prostitution were then introduced into Japan — a country where decriminalization was not realized in the dimension of criminal laws.

With its rapid economic growth, Japan became a consumer's society, in which the demand for prostitution increased. In response to this trend, more prostitutes committed financially compensated sexual acts in disguised brothels or in other various call‐girl systems. However, the total number of offenses of the Prostitution Prevention Law exposed by the police has decreased drastically during the past thirty years. In essence, we now see the de‐criminalization of prostitution in the practices of law enforcement agencies.