Application of DNA Forensic Techniques for Identifying Poached Guanacos (Lama guanicoe) in Chilean Patagonia*

Abstract: Guanaco (Lama guanicoe) is a protected and widely distributed ungulate in South America. A poacher, after killing guanacos in Valle Chacabuco, Chilean Patagonia, transported and stored the meat. Samples were retrieved by local police but the suspect argued that the meat was from a horse. Mitochondrial cytochrome b gene (774 pb), 15 loci microsatellites, and SRY gene were used to identify the species, number of animals and their population origin, and the sex of the animals, respectively. Analysis revealed that the samples came from a female (absence of SRY gene) Patagonian guanaco (assignment probability between 0.0075 and 0.0282), and clearly distinguishing it from sympatric ungulates (E‐value = 0). Based on the evidence obtained in the field in addition to forensic data, the suspect was convicted of poaching and illegally carrying fire arms. This is the first report of molecular tools being used in forensic investigations of Chilean wildlife indicating its promising future application in guanaco management and conservation.     

The Downside of Patriarchal Benevolence: Ambivalence in Addressing Domestic Violence and Socio-Economic Considerations for Women of Tamil Nadu,India

Social values and status cause diverse obstacles for escaping abuse (e.g., belief in the sanctity of marriage vs. financial necessity to stay for survival). India provides a unique opportunity to explore the interplay of status and corresponding patriarchal values in relation to the incidence of domestic violence and how it is viewed, coped with, and psychologically impacting native women. Sixty-four women of Tamil Nadu, India were surveyed. Women of higher status were found to be less likely to acknowledge abuse as a societal problem, accurately identify abuse events, and seek help or report abuse. Women who had more realistic conceptions of abuse were more likely to seek help but also likely to experience more severe psychological distress. All of the women surveyed had symptoms of post-traumatic stress disorder that were exacerbated by unsuspected variables. The implications of these findings are discussed in light of public health strategies.     

Detecting Specialization in Offending: Comparing Analytic Approaches

Offending specialization continues to be a subject of empirical inquiry for scholars interested in criminal careers. Early research consistently spoke to the generality of offending profiles, but more recent work has revealed somewhat mixed findings. These results have emerged alongside newly developed and applied methods that detect and describe offending specialization. To what extent these methods shape divergent conclusions and/or provide overlapping insight remains unclear, however. Therefore, the degree to which more recent inquiries are actually studying the same operational definition of specialization is unknown. In order to consider this issue further, this study utilizes four frequently applied approaches with a single data set. The study indicates when and where findings converge and also describes any unique insights provided by each method. The work concludes with a discussion surrounding the utility of applying multiple strategies in assessing specialization in criminal offending.     

无独立请求权第三人的诉讼地位

无独立请求权第三人是诉讼当事人。我国民事诉讼法关于无独立请求权第三人的立法规定存在着内在冲突,在理论和实务上产生了诸多令人困惑的问题,立法应明确无独立请求权第三人的诉讼当事人地位。当事人申请参加是无独立请求权第三人参加诉讼的适当方式,法院依职权追加和通知无独立请求权第三人参加诉讼,是超越司法被动性原则的体现,立法上应取消这一规定。     

Identification and analyses of female civilian victims of the 1991 war in Croatia from the Glina and Petrinja areas

Introduction

The process of recovering and identifying human remains from individual and mass graves has proven to be the most effective method of resolving the fate of missing individuals in the former Yugoslavia. These efforts have two primary objectives: medicolegal—to identify the cause of death, and humanitarian—to bring closure to living family members, thus supporting the human rights of both the living and the deceased. From 1996 to the present, the remains of 388 individuals killed during the 1991 War in Croatia were recovered from the Glina and Petrinja areas (Sisa?ko–Moslova?ka County). The purpose of this paper is to report on the demographic and taphonomic characteristics of the recovered remains, and identify factors responsible for the discrepancy in the identification ratios.

Methods

To identify factors potentially responsible for the noted discrepancy, data were collected on the taphonomic characteristics of the recovered remains, the types of burials, as well as the factors responsible for positive identification. These factors included: forensic DNA testing, dental criteria, special signs (healed fractures, etc.), clothing and personal artifacts.

Results

The age distributions between males and females in the sample are significantly different. The mean age-at-death for males is 51.6 years, for females 67.0 years. In both sexes the majority of identifications (62.9% in females and 45.9% in males) were established through a combination of biological and non-biological features. In terms of the types of graves from which the bodies were recovered, positive identification in both sexes was lowest in mass graves (61.2% for males, and 84.2% for females). Females from mass graves were, however, better preserved than males—36.6% of males recovered from mass graves were completely skeletonized, while the same was true for only 19.3% of females (χ² = 7.06, P < 0.029). Completely skeletonized remains were identified in only 65.1% of cases, while partially skeletonized and saponified bodies were identified in 80.1% of cases.

Conclusion

Since positive identification in both sexes is strongly correlated with preservation of the bodies, the reason why female identification frequencies in the Glina and Petrinja regions are higher than male frequencies lies in the fact that females recovered from mass graves were better preserved than males recovered from mass graves. Identification of the factors responsible for this is a challenge that needs to be resolved in future studies.     

Forensic application of Y chromosome SNPs in inconclusive cases

Biallelic markers, Single Nucleotide Polymorphisms (SNPs), are nowadays a powerful tool in the analysis of degraded samples. Namely, Y chromosome SNPs allow to determine the gender of the analyzed sample and to establish its haplogroup, making possible to attribute the ethnicity of male individuals. The aim of this study is to obtain Y-SNPs in forensic samples without STRs results, checking methodologies previously used.     

Forensic biological examination--the reality and perspectives

The point of view of authors on the modern condition and priority directions of the development of examination of biological material evidence is set out. The main idea consists in the necessarily of reorganization and correction of organization methodical base of examination of biological material evidence. The role and place in examination of material evidence of traditional serological and new--molecule genetic components are determined in this direction. It is noted that forensic biology must not ever be associated with only one of its part--serological methods of analysis. Under growing influence of molecule genetic component the semantic, and methodical content of forensic examination of material evidence. This is not already auxiliary differentiation of examination objects but conclusive set of their equality or difference. Thus forensic biological department of forensic medical examination Office must become expert compartments of new type due for complex analysis of material evidence where molecule genetic technologies lie in the centre. All other methodical compartments of forensic biology also will be used but as auxiliary and additional methods. In perspective it is necessary to enlarge and centralize of high-tech forensic molecule genetic laboratories based on common science-methodical approach. Unfavorable outcome of medical aid: study of the problem in forensic medicine practice