Forensic anthropology and the most probable cause of death in cases of violations against international humanitarian law: an example from Bosnia and Herzegovina

This study presents the results of the analysis of at least 298 predominantly male individuals, between 15 and 75 years, who were recovered from an open cast mine in Bosnia-Herzegovina. Particular attention is paid to identifying the mechanisms of injury and determination of the most probable cause of death based on the assessment of lethal or lethal-if-untreated injuries recorded in the skeleton. It was calculated that at least 38.9% (155/398) of individuals sustained gunshot wounds (GSWs) (plus one shrapnel wound) and may have died as consequence of these injuries. Among individuals who died from GSWs, there were 142 males (91.60%), eight females (5.1%) and five cases that were (3.2%) undetermined. One male individual sustained shrapnel injuries. This study presents an example of the multidisciplinary approach to the effective forensic investigation of violation against International Humanitarian Law, as well as an example of how it is possible to obtain meaningful results to assist the needs of the prosecution in these kind of cases despite the large number of cases and technological constraints.     

Genetic analysis of the Amerindian Kichwas and Afroamerican descendents populations from Ecuador characterised by 15 STR-PCR polymorphisms

Allele frequency data for the 15 STR systems and Amelogenine were determined in a population sample of healthy Amerinidian Kichwas and Blacks individuals. All loci met Hardy-Weinberg expectations and the high discrimination power of combined system showed the forensic efficiency of these genetic markers.     

Determination of several psychiatric drugs in whole blood using capillary gas-liquid chromatography with nitrogen phosphorus detection: comparison of two solid phase extraction procedures

A simple and reliable gas chromatographic method with nitrogen-phosphorus detection without derivatization was developed for the detection of several psychiatric drugs in whole blood as part of systematic toxicological analyses (STA). Drugs included mirtazapine, chlorpromazine, methotrimeprazine (levomepromazine), clothiapine, olanzapine, clozapine, haloperidol, and thioridazine. All drugs were studied at concentrations of 100-2,000 microg/L, except haloperidol that was studied at concentrations of 400-8,000 microg/L. In order to select the best blood purification procedure and therefore increase the signal to noise ratio we have compared two solid-phase extraction (SPE) columns, Chem Elut and Bond Elut Certify, for their recovery, precision, sensitivity and matrix purification efficiency. Recoveries for these drugs using Chem Elut columns at 500 and 2,000 microg/L (2,000 and 8,000 microg/L for haloperidol) were in the range 21-65%, with intra-assay and inter-assay precisions of less than 17% and 19%, respectively. Limits of detection (LODs) and limits of quantitation (LOQs) for mirtazapine, chlorpromazine, methotrimeprazine, clothiapine, olanzapine, clozapine, and thioridazine ranged from 62 to 161 microg/L and from 205 to 531 microg/L, respectively. LOD and LOQ for haloperidol were 442 and 1,458 microg/L, respectively. Recoveries of these compounds using Bond Elut Certify columns at 500 and 2,000 microg/L (2,000 and 8,000 microg/L for haloperidol) were in the range 44-97%, with intra-assay and inter-assay precisions of less than 7% and 14%, respectively. LODs and LOQs for mirtazapine, chlorpromazine, methotrimeprazine, clothiapine, olanzapine, clozapine, and thioridazine ranged from 37 to 66 microg/L and from 122 to 218 microg/L, respectively. LOD and LOQ for haloperidol were 156 and 515 microg/L, respectively. Linearity was observed in the studied range for all compounds with r(2) values of >0.999. The use of the mixed-mode bonded-silica Bond Elut Certify columns showed advantages comparing with Chem Elut columns for the screening of these psychotropic agents such as higher recoveries, cleaner extracts, better sensitivity, better precision and less solvent consumption and subsequent disposal.     

Postmortem vitreous humor beta-hydroxybutyrate: its utility for the postmortem interpretation of diabetes mellitus

Ketoacidotic coma is one of the most serious complications arising from diabetes mellitus, especially type I, and may be the cause of sudden death especially in diabetes type I. Since beta-hydroxybutyrate (beta-OHB) serum concentrations might provide more information on the severity of ketoacidosis, the aim of this study was to evaluate the concentrations of beta-OHB in vitreous humor and its correlation with other biochemical parameters during postmortem examination. We intended to ascertain the sensitivity and the specificity of these markers for diagnosing diabetes mellitus and the presence of ketoacidosis. This study involved 453 cadavers with a mean age of 57.6 years (S.D. 20.7) and a mean postmortem interval of 17.8 h (S.D. 9.6, range 2-61 h). Cases were assigned to two diagnostic groups according to the antemortem diagnosis of diabetes mellitus, based on the patients' medical records. In vitreous humor statistically significant differences were found in biochemical marker concentrations between the two diagnostic groups, the highest values being obtained in the group of subjects with a previous diagnosis of diabetes mellitus. The measurement of beta-OHB in vitreous humor may be a useful alternative to using blood during postmortem analysis. The presence of high levels of beta-OHB may help interpret the cause of death in diabetics when the autopsy result is negative.     

Lung embolism with liquid silicone

A lung embolism was reported in a case involving death following repeated injections of liquid silicone for aesthetic reasons. The liquid extracted from the sites of injection was identified as methylsilicone using infrared spectrophotometry, and the presence of silicone in vacuoles in the lung was verified by scanning electron microscopy with energy dispersive X-ray analysis (EDXA). A study has been carried out with rats after intravenous and subcutaneous injections of methylsilicone.     

Can experts judge elections? Testing the validity of expert judgments for measuring election integrity

Expert surveys have been used to measure a wide variety of phenomena in political science, ranging from party positions, to corruption, to the quality of democracy and elections. However, expert judgments raise important validity concerns, both about the object being measured as well as the experts. It is argued in this article that the context of evaluation is also important to consider when assessing the validity of expert surveys. This is even more important for expert surveys with a comprehensive, worldwide scope, such as democracy or corruption indices. This article tests the validity of expert judgments about election integrity – a topic of increasing concern to both the international community and academics. Evaluating expert judgments of election integrity provides an important contribution to the literature evaluating the validity of expert surveys as instruments of measurement as: (1) the object under study is particularly complex to define and multifaceted; and (2) election integrity is measured in widely varying institutional contexts, ranging from electoral autocracies to liberal democracies. Three potential sources of bias are analysed (the object, the experts and the context), using a unique new dataset on election integrity entitled the ‘Perceptions of Electoral Integrity’ dataset. The data include over 800 experts in 66 parliamentary and presidential elections worldwide. It is found that validity of expert judgments about election integrity is increased if experts are asked to provide factual information (rather than evaluative judgments), and if they are asked to evaluate election day (rather than pre‐election) integrity. It is also found that ideologically polarised elections and elections of lower integrity increase expert disagreement about election integrity. The article concludes with suggestions for researchers using the expert survey data on election integrity on how to check the validity of their data and adjust their analyses accordingly, and outlines some remaining challenges for future data collection using expert surveys.     

Economic Crisis and Support for Democracy in Europe

The study of the impact of the economic crisis on attitudes toward democracy tends to be focused on satisfaction with specific democratic institutions. This article expands upon previous research to explore how the current economic crisis can affect core support for democracy as a regime. Based on European Social Survey data for the Eurozone countries, the findings are twofold. It is shown, firstly, that perceptions of the state of the economy have an impact both on satisfaction with and support for democracy, and, secondly, that citizens’ support for democracy is greater in bailed-out countries. In countries that have experienced intervention, the more critical citizens and those less satisfied with the outputs of democracy are the stronger advocates of democracy. The article argues that this is connected with the tendency of critical citizens in bailed-out countries to blame external agents for the economic situation while increasing the saliency of democratic rules as a reaction to the imposition of unpopular measures.     

Y chromosome STR haplotypes in the Caribbean city of Cartagena (Colombia)

Haplotype data were obtained from a sample of 173 unrelated male individuals from Cartagena (Colombia), for 16 Y-chromosome STRs (DYS19, DYS385, DYS389 I, DYS389 II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS460, DYS461, DYS635, GATA H4 and GATA A10). No shared haplotypes were observed, demonstrating the usefulness and informative power of these Y-STRs in male lineage identification in Cartagena. Genetic distances were calculated using previously published haplotype data and the lowest values were found for the comparisons with samples of Iberian origin.