Commercialization activities in the SBIR program (part 1)

Telephone surveys were conducted over a three-year period on more than 800 Small Business Innovation Research (SBIR) Phase II projects initiated during the first three years of the program. For most of these projects, respondents were surveyed four years after receiving their Phase II award Five distinct levels of commercialization activity were identified ranging from “commercialization has occurred” (Level 1) to “commercialization is not expected” (Level 5). At the time of the survey, 12% of the projects had achieved commercialization (Level 1) and 18% were experiencing some commercialization success (Levels 1 and 2 combined). Of the participating federal agencies, the Department of Health and Human Services had by far the largest percentage of commercialized projects. For all agencies, over 60% of the respondents attributed nearly all of their projects' success to the SBIR program. Eighty-four percent stated that the technology development effort would not have been pursued without SBIR. Survey responses were also analyzed for a series of factors to determine their influence on the extent of commercialization activity. Factors included in the series are those related to the SBIR company conducting the project, the technology being developed, and marketing the product or service expected to be derived from the SBIR effort.     

Symbolism and the construction of political products: analysis of the political marketing strategies of peruvian President Alejandro Toledo

This paper introduces a discussion on how to construct and systematise symbols constructions that serve to define the guidelines for political discourses, using rhetorical methodology. It analyses the political marketing strategies of current Peruvian President Alejandro Toledo and, more specifically, his efforts to recover popularity after one‐and‐a‐half years in office. The fundamental hypothesis is that Toledo has not been able to construct a solid character (apart from being in opposition to Alberto Fujimori), who would be the protagonist in the ‘story’ of solving the real problems of the people, which, according to the opinion polls, are lack of employment, lack of opportunities, and lack of money for daily expenses. The rhetorical approach, based on Kenneth Burke's Dramatism Theory, is useful for understanding the construction of the political spectacle, the scene where all the marketing strategies and tools are applied. Copyright © 2004 Henry Stewart Publications     

Genetic admixture and diversity estimations in the Mexican Mestizo population from Mexico City using 15 STR polymorphic markers

The 15 AmpFlSTR Identifiler loci D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA were analyzed in a sample of 378 unrelated individuals from Mexico City, Mexico. Significant deviations from HW equilibrium in 14/15 STR loci alleles were not detected. The D18S51 locus had the highest power of discrimination (0.970). Genetic admixture estimations revealed a 69% of Amerindian, 26% of European and 5% of African contribution. Comparative analyses between Mexicans and other neighboring populations reveal significant differences in genetic diversity. Our results are important for future comparative genetic studies in different Latin American ethnic groups, particularly Mexican Mestizos and Amerindians. They should also be helpful in genetics, population evolution, forensic and paternity testing.     

Assignment of paternity in a judicial dispute between two neighbor Holstein dairy farmers

DNA profiling was used as evidence to assign paternity in a dispute between two neighbors in a judicial case of undue appropriation of cattle offspring from five alleged Holstein sires. Five offspring were genotyped using ten genetic markers (nine microsatellites and the BOLA-DRB3 locus). The computer program CERVUS was used to estimate the LOD score values and the confidence of paternity assignments. The results presented here show that three out of five paternity cases were assigned at 95% of confidence to a single sire with a LOD score ranging from 2.53 to 3.55. A fourth male was assigned using its delta value. Finally, all alleged sires were excluded from the paternity of the fifth offspring, probably due to the existence of an non-sampled male in the studied population. We concluded that the likelihood-based approach, included into CERVUS program, was a powerful tool in cattle kinship analysis when dealing with judicial dispute particularly when the dam's genotype was absent, allowing the assignments of paternity at 95% level of confidence in situations usually used by dairy and beef cattle producers in Argentine (e.g., multi-sire pasture mating).     

Quantitative analysis of multiple illicit drugs in preserved oral fluid by solid-phase extraction and liquid chromatography–tandem mass spectrometry

We present a validated method for the simultaneous analysis of basic drugs which comprises a sample clean-up step, using mixed-mode solid-phase extraction (SPE), followed by LC–MS/MS analysis. Deuterated analogues for all of the analytes of interest were used for quantitation. The applied HPLC gradient ensured the elution of all the drugs examined within 14 min and produced chromatographic peaks of acceptable symmetry. Selectivity of the method was achieved by a combination of retention time, and two precursor-product ion transitions for the non-deuterated analogues. Oral fluid was collected with the Intercept^®, a FDA approved sampling device that is used on a large scale in the US for workplace drug testing. However, this collection system contains some ingredients (stabilizers and preservatives) that can cause substantial interferences, e.g. ion suppression or enhancement during LC–MS/MS analysis, in the absence of suitable sample pre-treatment. The use of the SPE was demonstrated to be highly effective and led to significant decreases in the interferences. Extraction was found to be both reproducible and efficient with recoveries >76% for all of the analytes. Furthermore, the processed samples were demonstrated to be stable for 48 h, except for cocaine and benzoylecgonine, where a slight negative trend was observed, but did not compromise the quantitation. In all cases the method was linear over the range investigated (2–200 μg/L) with an excellent intra-assay and inter-assay precision (coefficients of variation <10% in most cases) for QC samples spiked at a concentration of 4, 12 and 100 μg/L. Limits of quantitation were estimated to be at 2 μg/L with limits of detection ranging from 0.2 to 0.5 μg/L, which meets the requirements of SAMHSA for oral fluid testing in the workplace. The method was subsequently applied to the analysis of Intercept^® samples collected at the roadside by the police, and to determine MDMA and MDA levels in oral fluid samples from a controlled study.     

Postmortem vitreous humor beta-hydroxybutyrate: its utility for the postmortem interpretation of diabetes mellitus

Ketoacidotic coma is one of the most serious complications arising from diabetes mellitus, especially type I, and may be the cause of sudden death especially in diabetes type I. Since beta-hydroxybutyrate (beta-OHB) serum concentrations might provide more information on the severity of ketoacidosis, the aim of this study was to evaluate the concentrations of beta-OHB in vitreous humor and its correlation with other biochemical parameters during postmortem examination. We intended to ascertain the sensitivity and the specificity of these markers for diagnosing diabetes mellitus and the presence of ketoacidosis. This study involved 453 cadavers with a mean age of 57.6 years (S.D. 20.7) and a mean postmortem interval of 17.8 h (S.D. 9.6, range 2-61 h). Cases were assigned to two diagnostic groups according to the antemortem diagnosis of diabetes mellitus, based on the patients' medical records. In vitreous humor statistically significant differences were found in biochemical marker concentrations between the two diagnostic groups, the highest values being obtained in the group of subjects with a previous diagnosis of diabetes mellitus. The measurement of beta-OHB in vitreous humor may be a useful alternative to using blood during postmortem analysis. The presence of high levels of beta-OHB may help interpret the cause of death in diabetics when the autopsy result is negative.     

Adaptation of the Narcissistic Personality Inventory among a Portuguese sample of incarcerated juvenile offenders

The aim of the present study was to assess the psychometric properties of the Narcissistic Personality Inventory (NPI) and two of its short forms (NPI-16 and NPI-13) among a Portuguese sample of male incarcerated juvenile offenders (N?=?221). Of these, only the NPI-13 short version demonstrated a good fit in terms of its factor structure. The NPI-13 also showed promising psychometric properties in terms of its internal consistency, convergent validity, discriminant validity, and criterion-related validity. Statistically significant associations were found with age of crime onset, crime seriousness, conduct disorder symptoms, alcohol abuse, and cannabis use. These findings justify the use of the NPI-13, especially its Entitlement/Exploitativeness dimension, among incarcerated male youths.     

The Syntax of Principles: Genericity as a Logical Distinction between Rules and Principles

Much has been said about the logical difference between rules and principles, yet few authors have focused on the distinct logical connectives linking the normative conditions of both norms. I intend to demonstrate that principles, unlike rules, are norms whose antecedents are linguistically formulated in a generic fashion, and thus logically described as inclusive disjunctions. This core feature incorporates the relevance criteria of normative antecedents into the world of principles and also explains their aptitude to conflict with opposing norms, namely that their consequents are fulfilled to varying extents more frequently than those of rules. I conclude that the property of genericity should be predicated to the norm antecedent of principles, more precisely to the hypothetical action. This is of paramount importance to explain, in terms of logical implication and exclusion, the expansibility of competing principles, in contrast with the exclusive character of conflicting rules.