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Trace DNA: a review,discussion of theory,and application of the transfer of trace quantities of DNA through skin contact 总被引:8,自引:0,他引:8
Wickenheiser RA 《Journal of forensic sciences》2002,47(3):442-450
Advances in STR PCR DNA profiling technology allow for the analysis of minute quantities of DNA. It is frequently possible to obtain successful DNA results from cellular material transferred from the skin of an individual who has simply touched an object. Handling objects, such as weapons or other items associated with a crime, touching surfaces, or wearing clothing, may represent sufficient contact to transfer small numbers of DNA bearing cells, or trace DNA, which can be successfully analyzed. With this minimal amount of contact required to yield a suspect profile comes tremendous crime solving potential, and a number of considerations for prudent application, and the maximization of evidentiary value. Evidentiary materials not previously considered must be recognized and preserved, and the resulting DNA type profiles interpreted in their proper forensic context. 相似文献
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The following analyte/isotope-labeled internal standard (IS) systems are adapted to further study the interference phenomenon previously reported from our laboratory--the intensity ratio of the ion-pair designated for a specific analyte/2H-analog system increases as the solvent used to reconstitute the extraction/derivatization residue is increased: (a) Three analyte/2H-analog pairs with 2H-atoms positioned at allylic sites (butalbital, secobarbital, methohexital); (b) Two analyte/2H-analog pairs without these structural features (pentobarbital, phenobarbital); and (c) Two analyte/13C-analog pairs (butalbital, secobarbital). Major experimental parameters adapted in this study include: (a) Varying reconstitution solvent volume while keeping a constant analyte/IS concentration ratio; (b) Varying analyte/IS concentration ratio; (c) Varying gas chromatograph (GC) injection port temperature; and (d) Varying GC column temperature programming conditions, rendering difference in the degree of overlap of the peaks derived from the analyte and the 2H-analog. This study results in the following observations: (a) Changes in the intensity ratio of the ion-pair designated for a specific analyte/2H-analog system depend on molecular abundance, regardless of whether the 2H-atoms are positioned at active allylic positions or not--thus, ruling out hydrogen/deuterium exchange as the cause of the observed interference phenomenon; (b) Variations in GC injection port temperature do not alter the observed interference phenomenon-thus, ruling out chemical reactions at the injection port as the underlying cause; (c) Variations in peak-overlapping between the analyte and the 2H-analog, facilitated by changing GC column programming conditions, alter the observed interference phenomenon. Abundance of the analyte and the 2H-analog and their overlapping characteristic in the mass spectrometer ion source are believed to be the underlying cause of the observed interference phenomenon. The interference phenomenon observed for a specific analyte/2H-analog system has significant consequences on the linearity of the thereby generated calibration curves. Nonlinear approaches can better describe the calibration data and are needed more in comparison to systems in which 13C-analogs are used as the ISs. 相似文献
84.
We previously wrote critically on the UK's welfare computerization programme of the 1980s and in this article we investigate the latest programme. Our argument is that welfare is being seen and is being understood in a technological context rather than a truly welfare one— that is, that the technology behind the computerization projects model views of how welfare recipients should be processed. In the 1980s, the computer model that was being applied was one of data processing (i.e. the 'business model'). In the 2000s, it is the model of computer-based communication (i.e. the 'Internet model'). But further, the new technological communication model is being effused with an ethical aspect— that those who do not communicate are lacking in socio-ethical responsibility to society, and are deemed not to be truly living up to their role as citizens. 相似文献
85.
In the UK and USA ‘Hate crime’ has become a topic of public controversy and social mobilization around issues of violence
and harassment. This has largely but not exclusively addressed racism, homophobia and gender based violence. This article
has three objectives. First, to situate hate crime legislation within a broad theory of modernity;secondly to examine the
politics of its emergence as a public issue; thirdly to use data from the authors' recent research in Greater Manchester to
illuminate the complexity of the concept of ‘hate crime’. The centrality of ‘hate crime’ to current debates derives from the
importance of rights-based regulation of complex societies and the juridical management of emotional life. Hatred and violence
have become problematic behaviour thrown into relief by a long term civilizing process. Hate crimes have thus acquired powerful
rhetorical focus for mobilization of victim and identity politics. With reference to racist violence in Oldham and elsewhere
in Greater Manchester, we argue that in its application and construction, however, ‘hate crime’ is a complex phenomenon that
might dramatize rather than regulate the problems it seeks to address.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
86.
Helping to sort the liars from the truth‐tellers: The gradual revelation of information during investigative interviews 下载免费PDF全文
Coral J. Dando Ray Bull Thomas C. Ormerod Alexandra L. Sandham 《Legal and Criminological Psychology》2015,20(1):114-128
Research examining detection of verbal deception reveals that lay observers generally perform at chance. Yet, in the criminal justice system, laypersons that have not undergone specialist investigative training are frequently called upon to make veracity judgements (e.g., solicitors; magistrates; juries). We sought to improve performance by manipulating the timing of information revelation during investigative interviews. A total of 151 participants played an interactive computer game as either a truth‐teller or a deceiver, and were interviewed afterwards. Game information known to the interviewer was revealed either early, at the end of the interview, or gradually throughout. Subsequently, 30 laypersons individually viewed a random selection of interviews (five deceivers and five truth‐tellers from each condition), and made veracity and confidence judgements. Veracity judgements were most accurate in the gradual condition, p < .001, η2 = .97 (above chance), and observers were more confident in those judgements, p < .001, η2 = .99. Deceptive interviewees reported the gradual interviews to be the most cognitively demanding, p < .001; η2 = .24. Our findings suggest that the detection of verbal deception by non‐expert observers can be enhanced by employing interview techniques that maximize deceivers' cognitive load, while allowing truth‐tellers the opportunity to respond to evidence incrementally. 相似文献
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