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71.
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Eleven Y specific microsatellites, previously studied in humans, were typed for fragment length and sequenced in chimpanzees (Pan troglodytes).The primers described by Ayub et al. (Nucleic Acids Res. 28, 2000, 2) for amplifying DYS434, DYS435, DYS436, DYS437, DYS438, DYS439 and those described by White et al. (Genomics, 57, 1999, 433) for GATA A10, A7.1, A7.2, C4, and H4, were used to amplify DNA samples from chimpanzees.Primers described for Y GATA A4 were found to amplify the same region as reported for DYS439. Moreover, the GATA A4 forward primer only matches the repeat flanking region in 14 of the 28bp, being responsible for a very weak amplification. Therefore, this system was not included in this study.The analysis of the repeat and sequence structure observed in chimpanzee and human Y chromosomes allowed evolutionary comparisons as well as the basis for improving Y STR nomenclature and therefore, a unified nomenclature for these novel STRs is proposed to the scientific community following ISFG recommendations.  相似文献   
73.
Abstract: Guanaco (Lama guanicoe) is a protected and widely distributed ungulate in South America. A poacher, after killing guanacos in Valle Chacabuco, Chilean Patagonia, transported and stored the meat. Samples were retrieved by local police but the suspect argued that the meat was from a horse. Mitochondrial cytochrome b gene (774 pb), 15 loci microsatellites, and SRY gene were used to identify the species, number of animals and their population origin, and the sex of the animals, respectively. Analysis revealed that the samples came from a female (absence of SRY gene) Patagonian guanaco (assignment probability between 0.0075 and 0.0282), and clearly distinguishing it from sympatric ungulates (E‐value = 0). Based on the evidence obtained in the field in addition to forensic data, the suspect was convicted of poaching and illegally carrying fire arms. This is the first report of molecular tools being used in forensic investigations of Chilean wildlife indicating its promising future application in guanaco management and conservation.  相似文献   
74.
The current environmental crisis calls for a sustainable style of development and draws attention to a new ecological dimension in social life that poses hitherto unforeseen challenges to the social sciences and to the everyday concerns of citizens, governments and private interests. Consequently, it is appropriate to attempt a critical overview of governance issues around the formation and implementation of environmental policies designed under the framework of sustainable development. After a brief introduction on how global perceptions about sustainable development have evolved, this article subsequently focuses attention on the main thrust of the emerging international environmental regime, and the multilateral environmental treaties embodied in it. Specific governance issues pertaining to environmental policies are also critically reviewed, particularly interrelations with the trade regime. The article concludes by spelling out some comments on research issues for the future, with a view to charting the environmental governance agenda lying ahead of us.  相似文献   
75.
What distinguishes studies of government (gouvernamentalité) from histories of administration, historical sociologies of state formation and sociologies of governance is their power to open space for critical thought. According to Michel Foucault, studies on government are studies of a particular stratum of knowing and acting, of the emergence of particular ‘regimes of truth’ concerning the conduct of conduct, ways of speaking truths and the costs of so doing, and of the inventing and assemblage of particular apparatuses and devices for exercising power and intervening in particular problems. The key point of this paper is that in the analytics of governmentality political freedom no longer depends on the systemic logic of the balance between government and governed, but on subjects’ obstinate and wild desire to live freely and on the ethos of those who intend to govern themselves and their like autonomously, which obstructs that logic even with extreme consequences. This capacity of resistance comes from life, from the sum of its functions that are useful in resisting death and no longer from a core of subjective rights, or from the will of individuals who oppose the state or the market.
Roberto CiccarelliEmail:
  相似文献   
76.
Taphonomy is the study of many variables involving decomposition, preservation, dispersal, erosion, burial or exposition of dead organisms. Forensic Taphonomy examines how biotic or abiotic variables can change evidences in legal investigations. Many insects are closely associated with decomposition processes. The scavenger dung-beetle, Coprophanaeus lancifer (Linnaeus, 1767), may be important biotaphonomically in the decomposition process of carcasses. Man-size pig carcasses were used as models to examine the decomposition process in the Adolpho Ducke Forest Reserve in central Amazonia. The scavenger dung-beetle has great potential in decomposition and production of post-mortem injuries, including dismemberment and the removal of soil beneath the carcass thereby causing a change in its position.  相似文献   
77.
Psychological pain has been reported to be major contributing factor for completed suicide, and classical suicidology based its wisdom on this construct. In this paper we report the case of a patient whose family experienced a high number of suicides apparently due to an escalation of psychological pain in the family members after the successive suicides. We also briefly discuss the problem of helping survivors after the suicide of loved one.  相似文献   
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79.
ABSTRACT

This article is a comparative analysis of two internationally awarded cases of Participatory Budgets in Canoas (Brazil) and Cascais (Portugal). Our main argument is that the success of these PBs relies on political/administrative, societal, and geographic drivers. This conceptual model has been adopted to analyse and discuss the success of the two case studies. The provision of an original conceptual model for comparative analysis and the empirical knowledge from the two cities are supported by the review of main literature in this field of study, and aim to contribute, with original findings, to the international debate on participatory budgeting.  相似文献   
80.
The Journal of Technology Transfer - Universities have become key elements in building regional innovation systems. However, even though academic research is important when firms choose...  相似文献   
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