鹿结核病流行株融合基因Rv3872-Rv3874-Rv3875的原核表达

以本实验室保存的鹿体内分离并鉴定的结核病流行株为模板,利用重叠延伸剪接技术设计融合基因的引物,扩增融合基因Rv3872-Rv3874-Rv3875,构建重组表达质粒pGEX-4T-1-Rv3872-Rv3874-Rv3875;重组表达质粒被转化入大肠杆菌BL21(DE3)后,IPTG诱导表达,并采用谷胱甘肽琼脂糖亲和层析方法对目的蛋白进行纯化。基因测序结果显示,融合基因Rv3872-Rv3874-Rv3875的序列与GenBank中序列的符合率为99.89%。SDS-PAGE分析显示,融合基因在大肠杆菌中成功被诱导表达,获得以可溶形式表达的融合蛋白,该蛋白的分子质量约为60ku。Western-blot鉴定结果显示,纯化好的融合蛋白能与鹿结核病的阳性血清发生反应,说明该融合蛋白具有良好的反应原性,这一结果为它在鹿结核病血清学诊断中的应用奠定了试验基础。     

Alleged Biological Father Incest: A Forensic Approach

Paternal incest is one of the most serious forms of intrafamilial sexual abuse with clinical, social, and legal relevance. A retrospective study was performed, based on forensic reports and judicial decisions of alleged cases of biological paternal incest of victims under 18 years old (n = 215) from 2003 to 2008. Results highlight that in a relevant number of cases: victims were female; the abuse begun at an early age with reiteration; the alleged perpetrator presented a history of sexual crimes against children; sexual practices were physically poorly intrusive, which associated with a forensic medical evaluation performed more than 72 h after the abuse, explain partially the absence of physical injuries or other evidence—these last aspects are different from extrafamilial cases. In conclusion, observations about paternal incest are likely to exacerbate the psychosocial consequences of the abuse and may explain the difficulty and delay in detect and disclose these cases. Few cases were legally prosecuted and convicted.     

Psychological and behavioral adjustment in female youths with high or low psychopathic traits

The aim of the present study was to analyze the role of psychopathic traits in female juvenile delinquency. Using a sample of 236 young females from the Juvenile Detention Centers of the Portuguese Ministry of Justice and schools in the Lisbon area, a group of female youths with high psychopathic traits (n = 118; M = 15.84 years of age; range = 14–18 years of age) and a group of female youths with low psychopathic traits (n = 118; M = 15.77 years of age; range = 14–18 years of age) were formed based on the Portuguese version of the Antisocial Process Screening Device-Self-report (APSD-SR). The results showed that young females with high psychopathic traits start engaging in criminal activities and come into contact with the justice system earlier in life; exhibit higher levels of behavioral problems, conduct disorder, delinquent behaviors and serious criminality; and demonstrate lower levels of self-esteem and pro-social behavior. The importance of some variables in predicting group membership (high versus low psychopathic traits) was established through a binary logistic regression. Our findings reinforce the importance of the psychopathy construct for the early identification of potentially high-risk female youths and for the assessment of female youths who have already come into contact with the judicial system.     

和谐与秩序: 亲属拒证权的理论之维简

刑事诉讼中赋予犯罪嫌疑人、被告人的亲属以拒证权是程序正义的内在要求,也是我国法律文化的优良传统与现代世界各国诉讼立法的普遍做法,但由于历史、政治等原因亲属拒证权一直未见于我国现行的刑事诉讼法。可以通过对东西方容隐法制的比较与分析,抽象出两者共通的和谐与秩序的价值追求,以期为当今中国建立起符合实际的亲属拒证权规则提供可资参评的理论支撑与根本理念。     

可持续发展视角下的地方环境资源保护法制建设研究——以黑龙江省大庆市为例

可持续发展是体现现代文明注重长远发展的一种经济发展模式,为保护地方所辖地域的环境资源,建设生态宜居型城市,可持续发展必须成为地方政府保障城市经济建设的基本战略方针。而针对地方环境资源目前所面临的严峻形势,建立健全与现阶段经济社会发展相适应的生态环境法制体系成为亟待解决的焦点问题。     

Interest groups in Brazil: a new era and its challenges

This article provides general and specific insights into Brazil's developing interest group system. In doing so, it presents a theoretical foundation for understanding this group activity, past and present. The general insights of the role of interest groups under limited political participation and authoritarian regimes down to the 1980s plus the period of democracy since then, provide background for the specific insights of the article. The specifics focus on three aspects of Brazil's contemporary interest group activity: (1) utilization of a neo-institutional analytical approach for understanding the interest group environment; (2) an analysis of the types of lobbying activity that takes place in Brazil today, including a case study; and (3) an assessment of the level of development of the group system by placing it in a comparative perspective with both advanced liberal democracies and other Latin American countries. The findings show that Brazil is, indeed, taking on many of the characteristics of a developed interest group system; but its past, its political culture, its political economy, and, paradoxically, its new-found status as an international power, work to present several challenges to its group system and thus to a full democratization of the country. Copyright © 2014 John Wiley & Sons, Ltd.     

量化分级管理制度信息公示的法理分析

量化分级管理制度作为卫生部监督管理餐饮服务行业和公共场所的一项重要举措已取得显著的成效,也成为我国建设法治国家、践行社会主义法治理念的一个具体着眼点。量化分级管理的信息公示作为一个重要的行政管理监管方法,有着其坚实的法理基础。本文从量化分级管理的信息公示与政府信息公开的关系入手,从主权在民和公民知情权这两个重要理论方面分析量化分级信息公示的法理内涵,为加快建立诚信社会,落实我国依法治国、执法为民、公平正义的法治理念做贡献。     

肝纤康A抗小鼠肝纤维化作用的拆方研究

目的：采用均匀设计法对肝纤康A进行拆方研究，筛选方中各药味的最佳配伍比例并确定优化组方(肝纤康F)。方法：以CCl4诱导小鼠肝纤维化模型，按均匀设计法安排实验，通过观察肝纤康A不同配比组方对小鼠肝组织羟脯氨酸(Hyp)含量和血清丙氨酸转氨酸(ALT)、天冬氨酸转氨酸(AST)活力的影响，并经均匀设计软件统计处理后确定最佳配比组方——肝纤康F。结果：通过拆方研究，确定优化组方为肝纤康F(90：75：24)。结论：经筛选优化的最佳配比在显著降低肝纤维化小鼠肝组织(Hyp)和血清(ALT)水平方面比原方更有效。     

Environmental conditions associated with shark attacks against humans: a case report about territoriality

This article describes the autopsy findings of a victim of a shark attack that took place off the coast of Pernambuco, Brazil. The victim was rescued and immediately taken to a nearby hospital but did not survive, even though the lapse of time between the attack and verification of death did not exceed 15 minutes. The presence of a single, large, contused and incised, semiarched wound with a serrated aspect was decisive in elucidating the event. Thus, this study discusses aspects related to the aggressor species, to the autopsy, to the type of wound, and to the causes that have led to a significant increase in this type of event in the area. It was concluded that the lesion was produced while the victim was still alive and that it was the cause of death.     

Mineralization of mandibular third molars can estimate chronological age--Brazilian indices

Forensic age estimation is an important element of anthropological research, as it produces one of the primary sources of data that researchers use to establish the identity of a person living or the identity of unknown bodily remains. The aim of this study was to determine if the chronology of third molar mineralization could be an accurate indicator of estimated age in a sample Brazilian population. If so, mineralization could determine the probability of an individual being 18 years or older. The study evaluated 407 panoramic radiographs of males and females from the past 5 years in order to assess the mineralization status of the mandibular third molars. The evaluation was carried out using an adaptation of Demirjian's system. The results indicated a strong correlation between chronological age and the mineralization of the mandibular third molars. The results indicated that modern Brazilian generation tends to demonstrate an earlier mandibular third molar mineralization than older Brazilian generation and people of other nationalities. Males reached developmental stages slightly earlier than females, but statistically significant differences between the sex were not found. The probability that an individual with third molar mineralization stage H had reached an age of 18 years or older was 96.8-98.6% for males and females, respectively.