Use of Embryos Extracted from Individual Cannabis sativa Seeds for Genetic Studies and Forensic Applications

Legal limits on the psychoactive tetrahydrocannabinol (THC) content in Cannabis sativa plants have complicated genetic and forensic studies in this species. However, Cannabis seeds present very low THC levels. We developed a method for embryo extraction from seeds and an improved protocol for DNA extraction and tested this method in four hemp and six marijuana varieties. This embryo extraction method enabled the recovery of diploid embryos from individual seeds. An improved DNA extraction protocol (CTAB3) was used to obtain DNA from individual embryos at a concentration and quality similar to DNA extracted from leaves. DNA extracted from embryos was used for SSR molecular characterization in individuals from the 10 varieties. A unique molecular profile for each individual was obtained, and a clear differentiation between hemp and marijuana varieties was observed. The combined embryo extraction–DNA extraction methodology and the new highly polymorphic SSR markers facilitate genetic and forensic studies in Cannabis.     

Travar el saber: Travesti-Centred Knowledge-Making and Education

This article focuses on what we call, following Argentinean thinker and activist Lohana Berkins, travar el saber. Travar el saber is travesti theorising; rooted in Berkins's conceptualisation of travesti as a political identity, it works to understand, question, and transform the tenets and practices that uphold and reproduce exclusionary dynamics. Our analysis draws on efforts taking place at the Bachillerato Popular Travesti y Trans Mocha Celis, the first high school created to meet the needs of travesti and trans students in Buenos Aires. The school seeks to create a travesti-centred transformative educational framework and praxis.     

The Adaptation of the FSLN: Daniel Ortega's Leadership and Democracy in Nicaragua

This article explores the capacity of the Frente Sandinista de Liberación Nacional (FSLN) to adapt to a changing Nicaraguan political environment over the last three decades. It focuses on the FSLN's transformation from the 1980s until its recent return to power. The analysis uses the tools offered by studies on the transformation and adaptation of political parties in adverse contexts. It concentrates on the four key stages of the FSLN's transformation: the 1980s, the five‐year period following the FSLN's defeat in the elections (1990–1995), the following decade in opposition (1996–2006), and the return to government. The key elements of the FSLN's adaptation relate to the centralization of party resources around the undisputed leadership of Daniel Ortega.     

Capital Punishment and Deterrence: Understanding Disparate Results

Objectives

Investigate how different model assumptions have driven the conflicting findings in the literature on the deterrence effect of capital punishment.

Methods

The deterrence effect of capital punishment is estimated across different models that reflect the following sources of model uncertainty: (1) the uncertainty about the probability model generating the aggregate murder rate equation, (2) the uncertainty about the determinants of an individual’s choice of committing a murder or not, (3) the uncertainty about state level heterogeneity, and (4) the uncertainty about the exchangeability between observations with zero murder case and those with positive murder cases.

Results

First, the estimated deterrence effects exhibit great dispersion across models. Second, a particular subset of models—linear models with constant coefficients—always predict a positive deterrence effect. All other models predict negative deterrence effects. Third, the magnitudes of the point estimates of deterrence effects differ mainly because of the choice of linear versus logistic specifications.

Conclusions

The question about the deterrence effect of capital punishment cannot be answered independently from substantive assumptions on what determines individual behavior. The need for judgment cannot be escaped in empirical work.     

Building Bond Repayment Capacity in Developing Countries: A Study on Property Tax Collections and Debt Affordability in Mexico

This article uses a multinomial regression model to analyze the bond repayment capacity of issuers of municipal bonds in Mexico. The study emphasizes the role that property and land-based taxes have in the enhancement of repayment capacity, as these are highly underutilized levies with important revenue raising potential. The findings show that there is no statistically significant link between these taxes and the chosen proxy for repayment capacity. This follows from an institutional and legal framework that creates an artificial environment of fiscal solvency. The Mexican case is instructive on how not to create a subnational bond market.