New alleles and mutational events at 14 STR loci from different German populations

The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci.     

An Experiment in “Practice to Theory” in Conflict Resolution

Negotiation Journal -     

What might be expected from the APEC meetings in Osaka

The purpose of the third APEC “summit,” taking place in Osaka on November 19, 1995, is to begin securing agreement on an action plan for achieving the “Bogor goals” of free and open trade and investment in the Asia-Pacific region by 2020, with the developed economies doing so by 2010. With pragmaticism as the hallmark of APEC deliberations, the leaders are expected (1) to focus on the principles of an action plan, leaving aside more difficult issues, (2) to conclude certain “business facilitation” agreements, and (3) to agree to accelerate the implementation of certain Uruguay Round agreements.     

Screening Biological Stains with qPCR versus Lateral Flow Immunochromatographic Test Strips: A Quantitative Comparison using Analytical Figures of Merit

Biological fluid identification is an important facet of evidence examination in forensic laboratories worldwide. While identifying bodily fluids may provide insight into which downstream DNA methods to employ, these screening techniques consume a vital portion of the available evidence, are usually qualitative, and rely on visual interpretation. In contrast, qPCR yields information regarding the amount and proportion of amplifiable genetic material. In this study, dilution series of either semen or male saliva were prepared in either buffer or female blood. The samples were subjected to both lateral flow immunochromatographic test strips and qPCR analysis. Analytical figures of merit—including sensitivity, minimum distinguishable signal (MDS) and limit of detection (LOD)—were calculated and compared between methods. By applying the theory of the propagation of random errors, LODs were determined to be 0.05 μL of saliva for the RSID? Saliva cards, 0.03 μL of saliva for Quantifiler^® Duo, and 0.001 μL of semen for Quantifiler^® Duo. In conclusion, quantitative PCR was deemed a viable and effective screening method for subsequent DNA profiling due to its stability in different matrices, sensitivity, and low limits of detection.     

Assessing the impact of EU governmentality in post-conflict countries: pacification or reconciliation?

European Union (EU) interventions in conflict countries tend to focus on governance reforms of political and economic frameworks instead of the geopolitical context or the underlying power asymmetries that fuel conflict. They follow a liberal pattern often associated with northern donors and the UN system more generally. The EU's approach diverges from prevalent governance paradigms mainly in its engagement with social, identity and socio-economic exclusion. This article examines the EU's ‘peace-as-governance’ model in Cyprus, Georgia, Palestine and Bosnia and Herzegovina. These cases indicate that a tense and contradictory strategic situation may arise from an insufficient redress of underlying conflict issues.     

Philemon and Baucis Deaths: Case Reports and Postmortem Biochemistry Contribution

The death of a person as a reaction to the death of another is known as Philemon and Baucis death and may represent a difficult challenge for pathologists, especially due to the decompositional changes that often characterized these situations. In this article, we describe two cases of Philemon and Baucis deaths, the first concerning a married couple and the second concerning a father and son. Postmortem investigations including radiology and biochemistry were performed. Based on the results of all investigations, deaths were attributed to natural causes. Third‐party involvement could be excluded in both situations. In one case, biochemical analysis results revealed potentially contributing causes of death. The reported cases emphasize the importance of correlating findings from different postmortem investigations to identify causes of death, precipitating conditions and predisposing disorders, thus allowing the conclusion of natural death to be reached and third‐party involvement to be excluded.     

Sequence variation and allele nomenclature for the X-linked STRs DXS9895, DXS8378, DXS7132, DXS6800, DXS7133, GATA172D05, DXS7423 and DXS8377

X-linked DNA markers are increasingly used in forensic kinship testing. This paper presents sequencing data of the short tandem repeats (STRs) DXS9895, DXS8378, DXS7132, DXS6800, DXS7133, GATA172D05, DXS7423, DXS8377 and proposes an allele nomenclature. Alleles were assigned according to the recommendations of the International Society of Forensic Genetics (ISFG) Commission.     

Peer Interaction and Engagement Through Online Discussion Forums: A Cautionary Tale

Following an very inspiring presentation from the REAP (Re-Engineering Assessment Practices in Scottish Higher Education) team, I attempted to address problems of attrition and non-engagement amongst first year law students by introducing online small group activities based around discussion forums into the level 4 (first year) Legal Method course. The idea was to build student engagement through teamwork online, thus moving towards a constructivist theory of learning (Jonassen et al. in Am J Distance Educ 9(2):7, 1995), and to increase formative feedback to students at an early stage in their course without unduly increasing my workload. (The benefits of early formative feedback are well documented. See, for example Yorke in Leaving early; Undergraduate non-completion in higher education, Falmer, London, 1999; Yorke and Longden in Retention and student success in higher education, SRHE and Open University Press, 2004). The students were randomly divided into groups of between 8 and 12 students using the WebCT group function. WebCT was at that time the university??s main virtual learning environment and available to all students enrolled on courses through the university portal system. Group discussion forums were provided for a series of activities, complementing the normal lecture pattern of two hours?? lecture plus one hour??s seminar each week. This paper considers the results over 2?years of introducing this system in a widening participation university. It did not prove possible to reproduce the success experienced in the REAP project. This paper attempts to unravel the various reasons for this, technical, pedagogical and practical. This case study is an example of action research, as I was both dismayed and intrigued by the negative reaction of the students and attempted to discover the reasons for it. The findings and conclusions drawn from them will be used to remodel this aspect of the course in 2010?C2011.