Highly efficient and automated extraction of DNA from human remains using a modified EZ1 protocol

Bones and teeth often represent the only sources of DNA available for identifying human remains. DNA in bones and teeth is generally better preserved than that in soft tissues because of the presence of hard connective tissue with a high level of calcium. Because of the extensive mineralisation, the choice of an efficient DNA extraction procedure is important to minimise the sampling of a high level of minerals and to remove polymerase chain reaction (PCR) inhibitors. Some protocols are available for DNA extraction from bones and teeth as part of the Qiagen EZ1 DNA Investigator Kit using the EZ1 Advanced XL automated purification platform. To improve the efficiency of DNA extraction from skeletal remains, the present study focuses on a modification to these already available protocols. In this study, different bones and teeth collected between 1 and 50 years after death were subjected to DNA extraction using the standard EZ1 protocol, a supplementary protocol, and a modified protocol. The modified approach included a decalcification step, whereas the Qiagen protocols worked directly on non-decalcified powder. In all three procedures, 150 mg samples were used for DNA extraction. We evaluated the quantity of DNA recovered from samples, the presence of any PCR inhibitors co-extracted, the level of DNA degradation, the quality of short tandem repeat (STR) profiles, and the reproducibility of the modified procedure. When compared with the other protocols, the modified protocol resulted in the best recovery of DNA that was free of PCR inhibitors. Additionally, the STR profiles were reliable and of high quality. In our opinion, the decalcification step increases DNA recovery by softening tissues, which allows lysis solutions to act more effectively. Furthermore, the use of two lysis solutions and the variation added to the EZ1 purification step allow for DNA recovery with quality and quantity superior to those of the previously available Qiagen-based protocols. These findings may be helpful solutions to the problems commonly encountered when dealing with difficult samples, such as bones and teeth.

Key points

• Bones and teeth often represent the only sources of DNA for identifying human remains.
• The choice of an efficient DNA extraction procedure is important for maximizing DNA recovery and removing PCR inhibitors.
• This study focuses on modifications to the previously available Qiagen-based protocols.
• The modified protocol enabled the best recovery of DNA, and both quality and quantity were superior to those of the previously available Qiagen-based protocols.
• The STR profiles obtained from samples extracted using the modified protocol were reliable and of high quality.

     

Development engagement with organised crime: a necessary shift or further securitisation?

Organised crime is increasingly being recognised as a development problem as well as a security threat. Underdevelopment creates a conducive environment for crime, while illicit flows undermine development progress. In response, development actors have begun to consider organised crime in their programming. However, there remains a reluctance to directly engage with organised crime, as there are fears that development will be further securitised. This has parallels with the reluctance of development actors to engage with conflict in the 1990s. This article draws on emerging reports from development agencies that begin to consider how organised crime can be addressed in development programming, comparing it to Goodhand's earlier framework on development and conflict. It evaluates what the different levels of engagement—working around crime, working in crime(-affected countries) and working on crime mean for the securitisation of development, assessing whether development engagement with organised crime is a necessary shift or further securitisation.     

Decomposition and insect succession of clothed and unclothed carcasses in Western Australia

The effect of clothing on carcass decomposition and patterns of insect succession onto remains were investigated in two separate years during autumn in Western Australia. The progression of decomposition differed between clothed and unclothed carcasses in both years of the study. The presence of clothing markedly prolonged the wet decay stage in both years with larval feeding occurring across the moist skin surface underneath clothing, as well as within and under the carcasses. Ambient temperatures were higher in the second year of the study and corresponded to marginally faster rates of decay throughout decomposition. Within years, insect arrival and oviposition were largely consistent between clothed and unclothed carcasses with a few notable exceptions. The green blow fly, Lucilia sericata Meigen (Diptera: Calliphoridae) oviposited one day earlier on clothed than unclothed carcasses in both years of the study. The black carrion fly, Australophyra rostrata Robineau-Desvoidy, (Diptera: Muscidae) colonised clothed carcasses in two distinct waves of succession but only one wave of ovipoistion was observed on unclothed carcasses in either year. Correspondingly, clothed carcasses supported larval feeding by A. rostrata for a longer duration than unclothed carcasses. Finally, dipteran larval masses were more widely distributed across the carcass surface and were present for a longer period of time on clothed carcasses than on unclothed carcasses in both years. Forensically relevant data detailing the seasonal pattern of insect succession onto clothed and unclothed decomposing remains in Western Australia are reported.     

Researching women's movements: An introduction to FEMCIT and Sisterhood and After

This article offers an overview of two research projects that are concerned with investigating the histories, social organisation and impacts of women's movements. It introduces FEMCIT (Gendered Citizenship in Multicultural Europe: the impact of contemporary women's movements), a transdisciplinary, cross-national European research project, and Sisterhood and After, a UK-based oral history project, outlining their specific research questions, foci and research designs. The article raises a number of key issues that arise in researching women's movements that are then taken up in the eight paired papers that follow: method and research design; difference and diversity; place, space and nation; and understanding impact.     

Queer Individualization: The Transformation of Personal Life in the Early 21st Century

This article explores what happens to understandings of social change in the realm of personal life when an empirical investigation is carried out that begins from rather different ontological and epistemological premises from those that have underpinned recent debates. It draws on UK‐based research that was framed by an engagement with sociological theories of individualization, psychoanalytically informed psycho‐social studies and queer theory, and that was designed to explore the psychic and affective dimensions, and the unconventional, counter‐heteronormative practices, of contemporary personal life. The study used the free association narrative interview method to examine the practices and ethics of personal life of people living outside conventional couples. It found considerable levels of psychic conflict and emotional distress, and some mental illness, amongst the people interviewed. Many interviewees told stories of experiencing a fracturing of self as they faced lives in which they felt alone and in which they were expected to be self‐responsible. These experiences, it is suggested, can be understood as tied up with losses contingent upon processes of individualization. However, the research also found evidence of a set of interrelated, counter‐heteronormative relationship practices that served reparatively to suture the selves undone by these processes of individualization: the prioritizing of friendship, the decentring of sexual/love relationships, and the forming of non‐conventional partnerships. The article proposes the notion of queer individualization to capture this set of transformations in the organization and experience of personal life, and suggests the necessity of understanding contemporary personal life as involving both the pain of loss, and new, reparative non‐conventional connections.     

The importance of being genuinely sorry when organizations apologize: how the Australian Wheat Board (AWB Limited) was damaged even further by its response to a corporate scandal

This paper draws on the unique insights offered by the documents tendered to the Royal Commission into the Australian Wheat Board (AWB Limited) to examine the process for drafting an apology within a scandalized organization suffering reputational damage. The Royal Commission received an almost complete set of documents specifying the process by which AWB prepared a public apology, as well as details of the efforts of the company to suppress those documents and the testimony of key personnel not only on the scandal itself but also on how the company responded to the crisis it had caused. The documents tendered to the Australian Government's Royal Commission included the working notes, drafting edits and expert advice from an international crisis communication consultant. AWB initially sought to try and manage the impact of the United Nations Oil‐for‐Food scandal, rather than address the causes of the transgression that would then allow the company to begin the process of restoring trust and rebuilding the damaged reputation. This disingenuous response exacerbated the problems for the company and caused additional damage to its corporate reputation. The insights from the AWB case study provide invaluable guidance for organizations on how to respond effectively to a corporate scandal. Copyright © 2012 John Wiley & Sons, Ltd.     

NSW Workers’ Compensation Reform: A Parody of Evidence‐based Policy Making

The characteristics and evolution of evidence‐based policy making in Australia and other mature democracies have been mapped extensively in this journal. This article advances research on the use of evidence in policy making, examining changes in the New South Wales workers’ compensation system from 2012 to 2014. This analysis of two phases of policy change, legislative implementation and statutory review, highlights the limitations of building integrated, coherent evidence‐based policy in a contentious policy area. The article finds that the collection of wide and detailed evidence will not satisfy requirements of evidence‐based policy without political will, transparency, and accountability.     

Sharing the struggle: constructing transnational solidarity in global social movements

ABSTRACT

Struggles for peace, self-determination and demilitarization are common near military installations around the world. Increasingly, these struggles have become linked in globe-spanning assemblages of activism. Based on interviews in South Korea, Okinawa, Puerto Rico, Hawai’i, and Guåhan (Guam) this paper analyzes how activists in these locales develop a sense of shared oppression that serves as a basis for connecting geographically distant activist communities. Through visiting each other’s places – and participating in activities such as direct action protests, eating together and dancing – activists develop a recognition of shared circumstance not only through intellectual discussion, but also through the production of shared visceral and emotional states. This shared feeling of mutual oppression then serves as a basis for solidarity and mutual aid among social movements that protest militarization and challenge traditional conceptualizations of security in international relations.