Electrospray-ionization MS/MS library of drugs as database for method development and drug identification

An ESI MS/MS library of 800 compounds has been developed and a collection of data is now available for Analyst 1.4 and higher. Compounds include forensically important drugs, such as illegal drugs, some deuterated analogues, hypnotics, amphetamines, benzodiazepines, neuroleptics, antidepressants and many others. For setting up the library of product ion spectra, 20-200 ng of the compounds have been injected either by flow injection or via a short LC-column, the precursor ions were chosen from the Q1 scan spectra, and product ion spectra were generated by CID in the collision cell using three different collision energies (20, 35 and 50 eV). Three spectra of each compound have been collected and compound names, CAS numbers, formulas and molecular weights have been added in the database, which has been generated by the Analyst software. The library can be used for compound identification during general unknown screening analysis by combination of Q1 scan techniques and subsequent MS/MS analysis in a second analytical run. Quantitative procedures for multi drug analysis using Multiple Reaction Monitoring can be established by selection of product ions and suitable collision energies from the library. For publication of the spectra, PDF-files have been generated and can be viewed on-line as supplementary data or from the website in alphabetical order: (supplementary data, should be made available via ELSEVIER-WEBSITE or via ).     

Subgaleal hematoma in child abuse

The mother brought her 31/2-year-old son to a paediatric clinic as his forehead was swollen and bluish in appearance. She was unable to give any explanation for this lesion. Clinically it was noted that a very visible and also palpable, doughy swelling was present both in the forehead as well as the hard skull areas. Some hours later, a massive bilateral periocular haematoma appeared. Whilst X-rays were unable to provide any diagnostic help, the sonographic examination of the skull carried out on the same day revealed an extensive subgaleal haematoma. This phenomenon is described in radiological literature as "scalping" and is caused by the use of considerable, blunt violence against the head (shear stresses, e.g. by pulling at the hair). The mother's boyfriend later confessed forceful pulling of the hair.     

Application of ultrasonography to postmortem examination. Diagnosis of pericardial tamponade

In postmortem examination, it is difficult to diagnose pericardial tamponade in a dead body from the findings of conventional external examination alone. However, ultrasonography is a common diagnostic tool for pericardial tamponade in clinical practice. We studied the postmortem diagnosis of pericardial tamponade at external examination level by applying an ultrasonographic device. The ultrasonographic findings were compared with the conventional autopsy findings. Among 455 cases of forensic autopsy in Hamburg and Tokyo conducted within 5 days after death, we successfully diagnosed 11 cases of pericardial tamponade by ultrasound imaging prior to autopsy, and failed to diagnose pericardial tamponade in only two cases. In addition, 79 cases of external examination conducted at the Tokyo Medicinal Examiner's Office were also examined with ultrasonography, and we diagnosed three cases of pericardial tamponade and five cases of pericardial effusion. The differences in ultrasonographic findings between tamponade and effusion were relatively clear. Although autopsy provides definitive evidence for the cause of death, sometimes autopsy cannot be performed due to some social factors. In such cases, conventional external examination alone cannot establish a cause of death by pericardial tamponade, and application of diagnostic imaging technique will be helpful. While CT and/or MRI may provide more detailed information than ultrasound imaging, these techniques require special equipment, room and specialist, and most of all involves high cost, which is perhaps the most important consideration in the present atmosphere of medical cost containment. On the other hand, the ultrasonographic devices we use are compact, and can be used directly at the scene of death. Postmortem application of ultrasonography may be a valuable adjunct in the work of medical examiners and forensic pathologists.     

Is Toxoplasma gondii a potential risk for traffic accidents in Turkey?

In Turkey, traffic accidents are the major causes of mortality and morbidity. According to the statistics made by Turkish Security Headquarters in the year 2000, 7500 people are killed in the traffic accidents every year. Drivers are mostly blamed for the traffic accidents. Tired, weary, sleepy and absentminded driving are common reasons for traffic accidents due to many reasons. Among these reasons mental and reflex conditions of drivers are significant. Toxoplasmosis is one of the most common zoonoses throughout the world. In immunocomponent adults, toxoplasmosis has no clinical signs or symptoms and infection in such people can only be detected by serological examinations. This kind of latent or dormant toxoplasmosis can be located in neural or muscular tissues and cause prolonged reaction times of the muscles. Extended reaction times also lead to deceleration of the reflexes which could be a major cause of the traffic accidents. By the light of this situation, the incidence of Toxoplasma gondii is investigated among the population who were involved in a traffic accident while driving. A total of 185 people (100 men and 85 women), aged between 21 and 40 years, living in Izmir and Manisa cities, were enrolled in the study group (SG) during a period of 6 months time. Their informed consents were taken initially and the laboratory tests of all these individuals related to blood alcohol levels after the accidents were found negative. The control group (CG) were also consisted 185 people (95 men and 90 women), residents of the same region, in same age group. The people in CG had no clinical signs or symptoms concerning toxoplasmosis. All collected sera were examined using a commercial IgG and IgM ELISA kit. According to the results of serological tests of the SG, 45 (24.32%) sera were IgG positive, six (3.24%) were IgM positive and 10 (5.40%) were both IgG and IgM positive. On the other hand in CG, 12 (6.48%) people found IgG positive, one (0.54%) found IgM positive and three (1.62%) found both IgG and IgM positive. This data was considered as statistically significant by Yates corrected chi2-test. (p < 0.05). In conclusion, there is an increased risk for traffic accidents for drivers owing to these high seroprevalence of latent toxoplasmosis. Prospective studies are still necessary on this subject but latent toxoplasmosis of drivers should be taken into account while developing strategies to prevent traffic accidents in Turkey.     

Overcoming Challenges to Using Evidence-Based Interventions in Schools

The Center for School Mental Health Assistance at the University of Maryland recently completed a review of evidence-based prevention and treatment programs that can be used by school mental health clinicians. Based on the review, a school-based program operating in 22 Baltimore City schools has purchased and trained clinicians in a number of protocols for evidence-based interventions. We present findings from this review and make pragmatic recommendations for school mental health programs to overcome the challenges associated with the use of evidence-based interventions.She received her doctorate in childclinical psychology from the University of Missouri-Columbia. Her research interests are in the area of the development, prevention, and treatment of juvenile delinquency and youth externalizing disorders.who does research in the area of childrens mental health services and the implementation of wraparound services in real-world settings.University of Maryland School of Medicine. His research is in the area of testing the effectiveness of School Mental Health Programs and the effects of community violence on youth mental health.involved in research related to school mental health, school-based health centers, and prevention of risky behavior among adolescents.Postdoctoral fellow at the time of the study.Postdoctoral fellow at the time of the study.     

Homicides with postmortal emasculation of the victim

Among criminal offences with mutilation of the genitals the number of cases with isolated removal of the testicles is small. This paper describes two homicides with postmortal emasculation of the victim from the autopsy material of the Berlin Institute of Legal Medicine (Humboldt University). In both of them no further mutilation had been committed. Yet the perpetrator's motivation was different in the two cases. In one case the cut-off testicles had been concealed in the victim's mouth, an act never described anywhere before. Obviously the offender wanted to mislead the police in their investigations. In the second case the motivation was of a homosexual nature and was thus comparable to previously described cases.     

Estimating eyeball protrusion from body height, interpupillary distance, and inter-orbital distance in adults

Eyeball protrusion is one characteristic that must be assessed/predicted in craniofacial identification methods of skull-face superimposition and facial approximation. Previously it has been suggested that average exophthalmometry values, as measured on living individuals, should be used. However, it is unknown if proptosis prediction can be improved beyond the accuracy obtained when using mean values. Some authors have suggested that relationships between exophthalmos, height, interpupillary distance, and interorbital distance exist and it has been reported that these latter variables can be used to estimate eyeball projection. However, crucial tests are yet to be conducted. This study measures these variables and tests the accuracy of exophthalmometry means, a previously proposed prediction equation, and newly derived regression equations to determine which methods provide the best results. Data indicate that variation in exophthalmos is fairly small and as such prediction from other variables, like body height, are weak; thus, exophthalmometry means currently offer the best practical method of prediction. It should be noted that up to 2 mm error from either side of the mean is expected for 68% of cases.     

Fatal blunt trauma of the larynx in a child

We report an autopsy case of an 11-year-old girl who suffered mechanical asphyxia from falling off the metal bars in the playground. This autopsy case is interesting because of the atypical trauma and lesions. To our knowledge there has been no similiar published case reports in the forensic literature. The young age of the victim, the setting and the pattern of the injuries are rare in a fall at playground by a child.     

Arbeiterbewegung von rechts? Motive und Grenzen einer imaginären Revolte

The article examines populist and völkisch orientations of unionized workers, some of them active members of workers’ councils. It empirically shows how, in respondents’ everyday consciousness, protest motifs are intermingled with an ethnicized view of the social question. Völkisch populism can be interpreted as a Polanyi-type movement that is motivated by problems generated by post-growth capitalism, presenting itself as a venture to give back power to the people. Its revolt remains an imaginary one, though, for, ultimately in accordance with existing power relations, it aims at reconstructing an irretrievable past. Our empirical results keep a distance from monocausal explanations, though call to mind issues of class that have long been neglected. As workers perceive the current distribution of wealth as unjust, yet don’t believe in any possibility for change, they are spontaneously inclined to redefine existing top-down conflicts into inclusion-exclusion types. Professional right-wing populists take up and aggravate this tendency of exclusive solidarity and thereby pose a serious challenge for the unions as well as for democratic civil societies.     

The state and the stray dogs in late Ottoman Istanbul: from unruly subjects to servile friends

The present article situates the systemic efforts to annihilate stray dogs within the wider picture of Ottoman modernizing reforms in the nineteenth century. The period under investigation witnessed an increasing desire on the part of the modern Ottoman state to control and reform disenfranchised human and animal groups, which were believed to jeopardize public order, security and hygiene. These groups – beggars, orphans and the unemployed – were identified as actors irreconcilable with the modern image that the reforming bureaucracy and modernizing elites sought to project. In the face of increasing challenges from European powers, they were the epitome of underdevelopment and backwardness. Ottoman elites and official authorities therefore proposed and implemented institutional measures in the form of forced labor, reformatories or deportation to reform the conditions of these groups, segregate them from the greater public and discipline them. In the modern period, along with the proposals that called for the removal of dogs, modernizing intellectuals and professionals proposed alternative plans to render non-human animals beneficial to human needs and the modern state's expectations.