Stable isotope analysis of human hair and nail samples: the effects of storage on samples

When submitting samples for analysis, maintaining sample integrity is essential. Appropriate packaging must be used to prevent damage, contamination or loss of sample. This is particularly important for stable isotope analysis by isotope ratio mass spectrometry as this technique is capable of detecting subtle differences in isotopic composition with great precision. In a novel study, scalp hair and fingernail samples were placed in five different types of packaging, routinely used in forensic laboratories and stored for 6 weeks and 6 months. Samples were subsequently cleaned and submitted for (13)C/(12)C, (15)N/(14)N, (2)H/(1)H and (18)O/(16)O analysis. Results from (13)C analysis indicate that type of packaging can cause slight changes in (13)C abundance over time. Differences were noted in the (15)N isotope signatures of both hair and nail samples after 6-week storage, but not after 6 months. This apparent discrepancy could be a result of the packaging not being properly sealed in the 6 weeks study. Fewer differences were noted when analyzing samples for (2)H and (18)O abundance.     

Urinary excretion profiles of 11-nor-9-carboxy-delta9-tetrahydrocannabinol and 11-hydroxy-delta9-THC: cannabinoid metabolites to creatinine ratio study IV

The objective of this study was to compare urinary excretion patterns of two cannabinoid metabolites in subjects with a history of chronic marijuana use. The first metabolite analyzed was nor-9-carboxy-delta9-tetrahydrocannabinol (delta9-THC-COOH), the major urinary cannabinoid metabolite that is pharmacologically inactive. The second metabolite 11-OH-delta9-THC is an active cannabinoid metabolite and is not routinely measured. Urine specimens were collected from four subjects on 12-20 occasions > or = 96 h apart in an uncontrolled clinical setting. Creatinine was analyzed in each urine specimen by the colorimetric modified Jaffé reaction on a SYVA 30R biochemical analyzer. All urine specimens analyzed for 11-OH-delta9-THC had screened positive for cannabinoids with the EMIT II Plus cannabinoids assay (cut-off 50 ng/mL) on a SYVA 30R analyzer and submitted for delta9-THC-COOH confirmation by GC-MS (cut-off concentration 15 ng/mL). Eleven-OH-delta9-THC was measured by GC-MS with a cut-off concentration of 3 ng/mL. Both GC-MS methods for cannabinoid metabolites used deuterated internal standards for quantitative analysis. The mean (range) of urinary delta9-THC-COOH concentration was 1153 ng/mL (78.7-2634) with a cut-off of 15 ng/mL. The mean (range) of delta9-THC-COOH/creatinine ratios (ng/mL delta9-THC-COOH/mmol/L creatinine) was 84.1 (8.1-122.1). The mean (range) urinary of 11-OH-delta9-THC concentration was 387.6 ng/mL (11.9-783) with a cut-off of 3 ng/mL, and the mean (range) of 11-OH-delta9-THC/creatinine ratio (ng/mL 11-OH-delta9-THC/mmol/L creatinine) was 29.7 (1.2-40.7). Of the 63 urine specimens submitted for delta9-THC-COOH confirmation by GC-MS, 59/63 urine specimens (94%) were positive for delta9 -THC-COOH and 51/63 (81%) were positive for 11-OH-delta9-THC. Overall, the concentrations of 11-OH-delta9-THC in urine specimens collected > or = 96 h apart were lower than delta9-THC-COOH concentrations in 50/51 of the urine specimens in this population. Further urinary cannabinoid excretion studies are needed to assess whether 11-OH-delta9-THC analyses have a role when assessing previous marijuana or hashish use in chronic users whose urine specimens remain positive for delta9-THC-COOH for an extended period of time after last drug use.     

Urinary excretion profiles of 11-nor-9-carboxy-Delta9-tetrahydrocannabinol: a Delta9-THC-COOH to creatinine ratio study #2

Subjects with a history of chronic marijuana use were screened for cannabinoids in urine specimens with the EMIT((R)) II Plus cannabinoids assay with a cut-off value of 50 ng/ml. All presumptively positive specimens were submitted for confirmatory analysis for the major urinary cannabinoid metabolite (Delta(9)-THC-COOH) by GC-MS with a cut-off value of 15 ng/ml. Creatinine was analyzed in each specimen as an index of dilution. Huestis and Cone [J. Anal. Toxicol. 22 (1998) 445] reported that serial monitoring of Delta(9)-THC-COOH to creatinine ratios in paired urine specimens collected at least 24h apart could differentiate new drug use from residual Delta(9)-THC-COOH excretion. The best accuracy (85.4%) for predicting new marijuana use was a Delta(9)-THC-COOH/creatinine ratio > or =0.5 (dividing the Delta(9)-THC-COOH to creatinine ratio of specimen 2 by the specimen 1 ratio). In a previous study in this laboratory [J. Anal. Toxicol. 23 (1999) 531], urine specimens were collected from chronic marijuana users at least 24h apart and dilute urine specimens (creatinine values <2.2 micromol/l) were excluded from the data analysis. The objective of the present study was to determine whether creatinine corrected urine specimens positive for cannabinoids could differentiate new marijuana use from the excretion of residual Delta(9)-THC-COOH in chronic users of marijuana based on the Huestis 0.5 ratio. Urine specimens (N=946) were collected from 37 individuals with at least 48h between collections. All urine specimens were included in the data review irrespective of creatinine concentration. The mean urinary Delta(9)-THC-COOH concentration was 302.4 ng/ml, mean Delta(9)-THC-COOH/creatinine ratio (ng/ml Delta(9)-THC-COOH/(mmol/l) creatinine) was 29.3 and the Huestis ratio calculation indicated new drug use in 83% of all sequentially paired urine specimens. The data were sub-divided into three groups (A-C) based on the mean Delta(9)-THC-COOH/creatinine values. Interindividual Delta(9)-THC-COOH/creatinine mean values ranged from 2.2 to 13.8 in group A (264 specimens, N=15 subjects) where 80.7% of paired specimens indicated new drug use. In group B, mean Delta(9)-THC-COOH/creatinine values ranged from 15.3 to 37.8 in 444 specimens (N=14 subjects) and 83.3% of paired specimens indicated new drug use. In group C, individual mean Delta(9)-THC-COOH/creatinine values were >40.1 (41.3-132.5) in 238 urine specimens (N=8 subjects) and 85.3% of paired urine specimens indicated new marijuana use. Correcting Delta(9)-THC-COOH excretion for urinary dilution and comparing Delta(9)-THC-COOH/creatinine concentration ratios of sequentially paired specimens (collected at least 48h apart) provided an objective indicator of new marijuana use in this population.     

Mandatory reporting by nurses of child abuse and neglect

Most Australian jurisdictions have mandatory reporting legislation to compel members of selected professional groups, including nurses, to report suspicions that a child has been or is likely to be subjected to abuse or neglect. This article details the legal obligations of nurses in each jurisdiction, and highlights differences between jurisdictions. Problematic features of the laws are identified, including the use of ambiguous concepts like "reasonable" suspicion and "significant" harm. Literature is reviewed to identify what is known about nurses' legal knowledge, actual reporting practice, and the practical problems that arise for nurses in this context. It is concluded that empirical research needs to be conducted, because it is not known if the laws are practically effective, whether nurses have sufficient training in, and knowledge of, their reporting duties, or what factors influence sound reporting. Such research can inform both the development of sound training systems and recommendations for legal reform.     

An attempt to reduce guessing behavior in children's and adults' eyewitness identifications

The effects of age of witness, gender of witness, lineup presentation, and practice on eyewitness testimony were investigated. Ninety-six elementary-school children and 96 college students viewed a slide sequence of a crime, followed by target-present or target-absent photo identification in sequentially or simultaneously presented lineups. Prior to photo identification, half the subjects received a practice lineup. Children had a higher rate of choosing than adults, resulting in more foil identification errors in both target-present and target-absent lineups. Without prior practice, sequential presentation as compared to simultaneous presentation reduced errors in target-absent lineups for adult witnesses and showed a similar but nonsignificant reduction for child witnesses. With prior practice, sequential presentation lost the advantage over simultaneous presentation in target-absent error reduction. Practice reduced target-absent errors in simultaneous-presentation lineups for both age groups.     

Book reviews

Gilles Deleuze and the Question of Philosophy PHILIP GOODCHILD London: Associated University Presses, 1996

Baudelaire and Schizoanalysis: The Sociopoetics of Modernism EUGENE HOLLAND Cambridge, Cambridge University Press, 1993

Australian Television and International Mediascapes STUART CUNNINGHAM & ELIZABETH JACKA Melbourne: Cambridge University Press, 1996

Sociolingwistics and Language History: Studies based on the Corpus of Early English Correspondence TERTTU NEVALAINEN & HELENA RAUMOLIN‐BRUNBERG (Eds) Language and Computers: Studies in Practical Linguistics No 15, Amsterdam/Atlanta., GA: Rodopi, 1996

Fear of the Dark. ‘Race’, Gender and Sexuality in the Cinema LOLA YOUNG London and New York: Routledge, 1996

Deleuze: A Critical Reader PAUL PATTON (Ed.) Oxford: Blackwell, 1996

A Guattari Reader GARY GENOSKO (Ed.) Oxford: Blackwell, 1996

Counterworks. Managing the Diversity of Knowledge R. FARDON (Ed.) London: Routledge, 1995     

Literary Reviews

Tyranny and redemption. Anthills of the Savannah. Chinua Achebe. London: William Heinemann. 1987. 233pp. £10.95hb

Suffering with stoicism: Kenyan histories. Coming to Birth. Marjorie Oludhe Macgoye. London: Virago Press. 1986. 150pp. £3.95pb

The Present Moment. Marjorie Oludhe Macgoye. London: Heinemann. 1987. 155pp. £10.95hb

The politics of the soul. Search Sweet Country. B Kojo Laing. London: Picador. 1987. 318pp. £3.95pb

Images of Istanbul. The Sea‐Crossed Fisherman. Yashar Kemal. Translated from the Turkish by Thilda Kemal. London: Methuen. 1986. 289pp. £3.95pb

The Birds Have Also Gone. Yashar Kemal. Translated from the Turkish by Thilda Kemal. London: Collins Harvill. 1987. 122pp. £8.95hb

Enemies within. War in the Land of Egypt. Yusuf al Qa'id. Translated from the Arabic by Olive and Lome Kenny and Christopher Tingley. London: Al Saqi. 1986. 192pp. £4.95pb

Frustrated fantasies. Heartbreak Tango: A Serial. Manuel Puig. Translated from the Spanish by Suzanne Jill Levine London: Arean. 1987. 224pp. £2.95pb

Pubis Angelical. Manuel Puig. Translated from the Spanish by Elena Brunet. London: Faber and Faber. 1987. 236pp. £9.95hb

Tending towards one voice. Faces and Masks. Eduardo Galeano. Translated from the Spanish by Cedric Belfrage. London: Quartet. 1987. 304pp. £14.95hb

Land of riches. Cuzcatlan. Manlio Axgueta. Translated from the Spanish by Clark Hansen. London: Chatto and Windus. 1987. 255pp. £11.95hb

Autobiography of the age. The Infinite Rehearsal. Wilson Harris. London: Faber and Faber. 1987. 88pp. £8.95hb

Caribbean childhoods. Summer Lightning and Other Stories. Olive Senior. Harlow, England: Longman. 1986. 134pp. £2.95pb

Whole of a Morning Sky. Grace Nichols. London: Virago. 1987. 156pp. £3.50pb

Traumas of partition. Kingdom's End and Other Stories. Saadat Hasan Manto. Translated from the Urdu by Khalid Hasan. London/New York: Verso. 1987. 257pp. £U.95/S18.95hb

Mirroring Pakistan: reflections and distortions. The Thirteenth House. Adam Zameenzad. London: Fourth Estate. 1987. 202pp. £10.95hb

Black Mirrors. Farooq Khalid. Translated from the Urdu by Eric Cyprian. London: Jonathan Cape. 1987. 285pp. £10.95hb

Christopher Shackle. School of Oriental and African Studies, London. Prize poetry. Under Another Sky: An Anthology of Commonwealth Poetry Prize Winners. Edited by Alastair Niven. Manchester and New York: Carcanet. 1987. 103pp. £6.96/S10.95pb     

Can society be commodities all the way down? Post-Polanyian reflections on capitalist crisis

Abstract

In his classic 1944 book, The great transformation, Karl Polanyi traced the roots of capitalist crisis to efforts to create ‘self-regulating markets’ in land, labour and money. The effect was to turn those three fundamental bases of social life into ‘fictitious commodities’. The inevitable result, Polanyi claimed, was to despoil nature, rupture communities and destroy livelihoods. This diagnosis has strong echoes in the twenty-first century: witness the burgeoning markets in carbon emissions and biotechnology; in child-care, schooling and the care of the old; and in financial derivatives. In this situation, Polanyi's idea of fictitious commodification affords a promising basis for an integrated structural analysis that connects three dimensions of the present crisis: the ecological, the social and the financial. This paper explores the strengths and weaknesses of Polanyi's idea.