Oral fluid testing for cannabis: on-site OraLine IV s.a.t. device versus GC/MS

Saliva or "oral fluid" has been presented as an alternative matrix to document drug use. The non-invasive collection of a saliva sample, which is relatively easy to perform and can be achieved under close supervision, is one of the most important benefits in a driving under the influence situation. Moreover, the presence of Delta9-tetrahydrocannabinol (THC) in oral fluid is a better indication of recent use than when 11-nor-Delta9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) is detected in urine, so there is a higher probability that the subject is experiencing pharmacological effects at the time of sampling. In the first part of the study, 27 drug addicts were tested for the presence of THC using the OraLine IV s.a.t. device to establish the potential of this new on-site DOA detection technique. In parallel, oral fluid was collected with the Intercept DOA Oral Specimen Collection device and tested for THC by gas chromatography mass spectrometry (GC/MS) after methylation for THC (limit of quantification: 1 ng/mL). The OraLine device correctly identified nine saliva specimens positive for cannabis with THC concentrations ranging from 3 to 265 ng/mL, but remained negative in four other samples where low THC concentrations were detected by GC/MS (1-13 ng/mL). One false positive was noted. Secondly, two male subjects were screened in saliva using the OraLine and Intercept devices after consumption of a single cannabis cigarette containing 25mg of THC. Saliva was first tested with the OraLine device and then collected with the Intercept device for GC/MS confirmation. In one subject, the OraLine on-site test was positive for THC for 2 h following drug intake with THC concentrations decreasing from 196 to 16 ng/mL, while the test remained positive for 1.5 h for the second subject (THC concentrations ranging from 199 to 11 ng/mL). These preliminary results obtained with the OraLine IV s.a.t. device indicate more encouraging data for the detection of THC using on-site tests than previous evaluations.     

Alcohol metabolism of local Chinese in Hong Kong: a statistical determination on the effects of various physiological factors

A study was designed to examine the elimination rate of alcohol from the body of the local Chinese after consumption of different types of alcoholic drinks. The breath alcohol of 184 healthy volunteers was determined and converted into blood alcohol levels after they finished drinking. Information on the type and volume of alcoholic drinks consumed, age group, sex, drinking habit, and drinking on empty stomach or with/after meal was recorded for each participant. The results show that the elimination rate of an individual can be explained in terms of physiological variables including sex and drinking habit. The determined elimination rates allow forensic toxicologists to back calculate the blood alcohol concentration (BAC) of the drivers at the time of accident in drunk driving cases. The elimination rates of blood alcohol at 95% prediction intervals for male and female are in the range of 9.5-23.8 mg/100 ml/h and 11.1-37.1 mg/100 ml/h, respectively.     

Homicide by decubitus ulcers

Traditionally, the only penalties for poor treatment of nursing home patients have been civil lawsuits against nursing homes and their employees by families, or fines and license suspension by government organizations. Recently, government agencies have become much more aggressive in citing institutions for the development of decubitus ulcers (pressure sores) in their patients. A few government institutions have concluded that in some cases, the development of ulcers with resultant death is so grievous that there should be criminal prosecution of the individuals and/or institutions providing care. A leader in this concept has been the State of Hawaii. In November 2000, the State of Hawaii convicted an individual of manslaughter in the death of a patient at an adult residential care home (a form of nursing home) for permitting the progression of decubitus ulcers without seeking medical help, and for not bringing the patient back to a physician for treatment of the ulcers.     

Symmetrical fracturing of the skull from midline contact gunshot wounds: reconstruction of individual death histories from skeletonized human remains

This paper reports a bilaterally symmetrical cranio-facial fracture pattern that is observed in self-inflicted, midline gunshot wounds. Five cases of self-inflicted gunshots wounds are presented as follows: two high-powered rifle cases, two shotgun cases, and one handgun case. In all five cases the remains were either decomposing or skeletonized and submitted to forensic anthropologists. Following identification, the main focus of the anthropological examination was the analysis of perimortem trauma to the skeleton. In each case, the skull was submitted in a highly fragmented state. Nevertheless, by focusing on the pattern of perimortem cranio-facial fractures, the anthropologists contributed key information regarding the circumstances of death. The observed symmetrical cranio-facial fracture patterns in the above cases are described in detail and interpreted. The specific location of the linear fractures is discussed, as well as the theoretical rationale behind the location in terms of skeletal architecture, such as buttresses, struts, and sutures. The interpretive framework provided by this paper may prove helpful to others who are faced with similar cases of cranio-facial fracturing.     

Testing for GHB in hair by GC/MS/MS after a single exposure. Application to document sexual assault

Gamma-hydroxybutyric acid, or GHB, is a substance naturally present within mammal species. Properties of neurotransmitter or neuromodulator are generally given to this substance. GHB is therapeutically used as an anesthetic, but can be used for criminal offenses (date-rape drug). It appears that the window of detection of GHB is very short in both blood and urine, and therefore its presence is very difficult to prove after a rape case. In order to document single exposure, we investigated the use of hair. Hair was collected one month after the allegated event in order to sample the corresponding period after regular growing. After rapid (2 min) decontamination with dichloromethane, the hair shaft was cut into 3-mm segments. They were overnight incubated in 0.01 N NaOH in the presence of GHB-d6, followed by neutralization and extraction in ethyl acetate under acidic conditions. GHB (precursor ion m/z 233, product ions m/z 147 and 148) was tested by GC/MS/MS (Finnigan TSQ 700) after derivatization with BSTFA + 1% TMCS. Physiological concentrations (n = 24) were in the range 0.5 to 12.0 ng/mg, with no influence due to hair color. No variation of concentrations was observed along the hair shaft in controlled subjects, except for the proximal segment, due to an incorporation through sweat. This demonstrates that endogenous levels for each single subject are constant during hair growth. A controlled human administration of 25 mg/kg to a volunteer demonstrated that a single exposure to GHB is detectable in hair after segmentation. In a case of rape under influence, a clear increase of the corresponding segment (about 2.4 ng/mg) in time was observed, in comparison with the other segments (0.6 to 0.8 ng/mg). This study demonstrates that a single exposure to GHB in a case of sexual assault can be documented by hair analysis when collected about one month after the crime.     

Where to Begin?

If what goes on in human societies are viewed as "institutionalfacts"rather than "brutefacts," then basic issues are raised withregard to the meaning of federalism. Institutional facts areconstituted by reference to rules and rule-ordered arrangements.Such arrangements turn upon design concepts and their placein the constitution of order in human societies. Constitutingsocieties through patterns of covenantal relationships impliesthat federal societies cannot be understood by placing primaryreliance on forms of government. The question "where to begin?"raises basic ontological and epistemological problems bearingon the place of language and culture in the constitution ofcovenanting societies.     

L'information administrative au Québec: faits et interprétations

Sommaire. L'information administrative. dans nos sociétés. sodre de failles diverses. Une recherche, faite pour le compte du Ministère des Communications du Québec, a voulu éclairer ces failles en vue de les corriger. La principale composante de la recherche a consisté en 78 entrevues de groupe conduites auprès de hauts-fonctionnaires, de fonctionnaires de contact, et de clients privilégés de différentes entités administratives. De plus, 17 experts de différentes disciplines se réunirent pendant deux jours avec des fonctionnaires du Ministère des Communications et d'autres ministères pour tenter d'interpréter et de situer dans des contextes plus larges les problèmes de l'information administrative. Après avoir défini l'information administrative d'après un schéma de la communication empruntéà Roman Jakobson, l'article fait état des principales constatations qui ont été faites lors de la recherche. Ensuite sont présentées les diverses interprétations proposées par les experts: interprétations psychologiques, interprétations en termes de techniques de la communication, interprétations micro-économiques, et interprétations en termes de pouvoir. Les forces et les faiblesses de ces interprétations sont évaluées, après quoi est esquissée une analyse structurale, où l'insistance est mise sur la capacité qu'ont les acteurs d'imposer les contacts par où passe l'information administrative. Il est montré en conclusion comment cette analyse structurale permet d'intégrer les interprétations proposées par les experts, et comment elle pourrait être améliorée dans des recherches subséquentes. Abstract. Administrative information, in our societies, suffers from various shortcomings. A research project undertaken on behalf of the Quebec Department of Communications has tried to focus on these weaknesses in order to eliminate them. The main element of the research was 78 group interviews with senior officials, ‘contact’ civil servants, and privileged clients of various administrative entities. Furthermore, 17 experts in various disciplines met for two days with officials of the Department of Communications and other departments in an attempt to interpret and place in broader contexts the problems of administrative information. Administrative information having been defined according to a communication grid borrowed from Roman Jakobson, this article states the various facts established by the research. Then, the various interpretations offered by the experts are presented: psychological interpretations, interpretations in terms of communication techniques, micro-economic interpretations, and interpretations in terms of power. Strengths and weaknesses of these interpretations are evaluated, and then a structural analysis is attempted; it focuses on the actors' ability to impose the contacts channelling administrative information. In conclusion it is shown how this structural analysis allows an integration of the interpretations proposed by the experts and how it could be improved with further research.