The Reliability of Chronological Age Determination by Means of Mandibular Third Molar Development in Subjects in Croatia*

Abstract: The aim of this study was to determine the development of the mandibular third molar and its relation to chronological age. The evaluated material consisted of 979 orthopantomograms of patients of Croatian Caucasian origin aged between 5.7 and 14.6 years. Third molar developmental stages were evaluated according to the stages proposed by Nolla. The frequencies of different stages of mineralization and the mean value of the mineralization of the mandibular third molars with regard to age, gender, and size of the mandible were determined and the coefficient of correlation determined between the age of the subject and the stage of development of the mandibular molars. Statistically significant correlation was determined between mineralization of the mandibular third molar and chronological age of the subjects (boys, mandibular left third molar r = 0.779, right third molar r = 0.793; girls, mandibular left third molar r = 0.746, right third molar r = 0.725). It can be concluded that the accuracy of age estimation based on Nolla’s method is applicable for Croatian children.     

Investigating the legal protection of data,information and knowledge under the EU data protection regime

Information science distinguishes between the semantic forms/intangibles of data, information and knowledge. Data (e.g. an attribute of a data record in a relational database) does not have any meaning by itself. Information is data brought into context (e.g. data related to its primary key), and knowledge is the collection of information for useful intent (e.g. a database). This paper investigates the mapping of semantic forms in information science (i.e. data, information, knowledge) to correlative concepts in information law (primarily data protection legislation) with a view to investigating how such semantic forms are legally protected. The paper first proposes a data, information, knowledge, rules (DIKR) hierarchy in the context of relational database theory, and interprets this hierarchy with respect to data protection concepts. The paper then gives an in-depth discussion of the elements of the DIKR hierarchy (data, information, knowledge, deduced knowledge, induced knowledge) and how they relate to the EU Data Protection Directive 95/46/EC. These relationships are summarized in the form of a two dimensional correlation matrix. Finally the paper discusses how the semantic forms identified are protected under the EU Data Protection Directive, and gives insightful observations about the connection between information law and information science.     

Lessons learned from inter-laboratory studies of carbon isotope analysis of honey

Forensic application of carbon isotope ratio measurements of honey and honey protein to investigate the degree of adulteration with high fructose corn syrup or other C₄ plant sugars is well established. These measurements must use methods that exhibit suitable performance criteria, particularly with regard to measurement uncertainty and traceability – low levels of adulteration can only be detected by methods that result in suitably small measurement uncertainties such that differences of 1‰ or less can be reliably detected. Inter-laboratory exercises are invaluable to assess the state-of-the art of measurement capabilities of laboratories necessary to achieve such performance criteria. National and designated metrology institutes from a number of countries recently participated in an inter-laboratory assessment (CCQM-K140) of stable carbon isotope ratio determination of bulk honey. The same sample material was distributed to a number of forensic isotope analysis laboratories that could not participate directly in the metrological comparison. The results from these studies have demonstrated that the majority of participants provided isotope delta values with acceptable performance metrics; that all participants ensured traceability of their results; and that where measurement uncertainties were reported; these were fit-for-purpose. A number of the forensic laboratories only reported precision rather than full estimates of measurement uncertainty and this was the major cause of the few instances of questionable performance metrics. Reporting of standard deviations in place of measurement uncertainties is common practice outside metrology institutes and the implications for interpretations of small differences in isotopic compositions are discussed. The results have also highlighted a number of considerations that are useful for organisers of similar inter-laboratory studies in the future.     

Families and households of the poor: The 19th-century Slovenian gostači

The present study examines the position of day laborers, a social category still awaiting comparative study by family historians. In 19th-century Slovenian sources these laborers, called gosta?i, were a very mobile group that did not own property; they could be married or single and could be involved in various occasional jobs, agricultural or industrial, skilled or unskilled. By comparing two communities (one agrarian, the other centered on iron production), the study shows that the living and working arrangements of gosta?i were profoundly affected by the economy and by natural resources. They therefore developed different residential patterns, though in neither case did their families constitute a part of the owner's sub-household in the Laslettian sense. The study concludes that in the 19th century this poor social group should not be treated as a homogenous workforce but as flexible and dynamic.     

Viability of Human Articular Chondrocytes Harvested Postmortem: Changes with Time and Temperature of In Vitro Culture Conditions

Different studies of long‐term chondrocytes viability have shown a gradual reduction as a function of time and ambient temperature. The aim of our in vitro study was to establish chondrocyte postmortem viability curves for 4°C, 11°C, 23°C, 35°C during 63 days after the donors' death. Osteochondral cylinders were procured from the knees of 16 male donors (20–47 years), stored in preservation media that was not changed, and analyzed in 3‐day intervals using a confocal laser scanning microscope. A significant influence of time on viability was found from Day 9 (p = 0.0029) and onwards (p < 0.0001). The lowest overall chondrocyte viability was at 35°C, followed by 4°C (p < 0.0001). The conditions used in this in vitro analysis suggest that similar viabilities may occur while in situ in the decedent. Further studies of chondrocyte viability from individuals with known postmortem intervals may show premise to help evaluate time since death in the late postmortem interval.