Induction of hypoxia-inducible factor-1alpha in two kinds of rats asphyxiation death models

OBJECTIVE: To investigate the expression of hypoxia-inducible factor 1-alpha (HIF1-alpha) in the heart, lung, liver and kidney in rats died of two typical models of asphyxia. METHODS: Two asphyxia models were made and tissue samples of the dead rats were collected from different groups at various postmortem duration. The expression and the changes of HIF1-alpha in various tissues were examined by immunohistochemistry and image analysis techniques. Results Significant expression of HIF1-alpha was observed in the myocardial fibers, kidney cells, liver cells and lung cells in both asphyxia models, but not in the control group. The expression of HIF1-alpha in various tissues in the rat died of nitrogen gas breathing was found in the nuclei at 0 hour and the expression level decreased gradually thereafter. The HIF1-alpha expression level and duration in various tissues of the rat died of hanging were higher and longer than that of the former group, with a peak of the expression level observed 6 hours after death, and then started to decline in all tissues except the heart where the expression still showed an increase 24 hours after death. The control groups showed a steady expression in the cytoplasm but not in the nuclei. CONCLUSION: HIF1-alpha appears to be a valuable biomarker in the diagnosis of asphyxia within 24 hours after death.     

论我国行政征收补偿程序的不足及完善

行政征收是指行政主体为了公共利益的需要,按照法定的程序,以强制方式取得私人不动产的所有权或其他物权的行为.我国的行政征收补偿程序主要分为土地征收补偿和城市房屋征收补偿.但目前我国的行政征收补偿程序仍存在诸多问题,如征收目的不明确,征收程序启动较随意化,征收补偿缺乏统一而明确的程序规则等,需要进一步完善.     

马克思主义哲学中国化研究的视野与方法

马克思主义哲学是被实践证明了的科学的世界观和方法论,马克思主义哲学中国化进行的好与不好,顺利或不顺利,直接关乎到马克思主义在中国能否实现真正的中国化———富有中国作风和中国气派,反映民族性和时代性。当前马克思主义哲学中国化过程中面临的种种困境和挑战,为一种新的研究视野与研究方法的提出提供了可能性。站在世界历史的视野,采用历史主义研究方法,马克思主义哲学中国化研究这种在视野与方法上的创新,有助于进一步推动马克思主义中国化。     

社会转型期公众民意表达科学化的理性思考

当前,我国正处于社会转型深化期和攻坚期,公众民意表达发生了深刻变化,呈现出利益诉求不断扩大的趋势,突出表现为多元化、差异化和群体化等新特点。在面对公众民意表达时,政府与公众应在公共理性的框架内,寻求理性和有序的解决途径。为提升公众民意表达的科学化水平,必须加强和创新社会管理,大力营造党委、政府、社会和公众理性互动的社会管理环境,创新发展治理型的社会管理制度,不断拓宽政府主导的规范高效、理性有序的社会管理路径。     

经济法视域中的软法规范刍议

随着世界范围内国家管理的衰落与公共治理的兴起,将法分为硬法和软法两种基本形式并非没有根据,对软法规范的研究也日益引起学界的浓厚兴趣。事实上,经济法作为一种回应型法,软法规范大量存在并具有非国家强制性、创制方式的弹性与民主性、非司法中心主义、法律位阶的非层次性等特点,使得其与经济法视域中的硬法规范区别开来。同时它与经济法视域中的硬法规范相得益彰,共同规范和调整着经济法关系。但软法规范的缺陷也是极其明显的,软法的泛化不利于法治社会的构建。为此,有必要将软法规范赋予硬法的某些特征使软法朝着硬法化方向转化;增强某些软法规范的刚性使其朝着可诉化方向迈进。     

On the Legal Foundation and Value Function of Sunshine Discipline Inspection

阳光检务是检务公开的深化,是公正检务、高效检务、廉洁检务.阳光检务的法理基础是公民知情权理论、权力制约理论和程序正义理论.阳光检务具有指引、评价、教育、预测和保障等规范功能,以及促进检察权依法正确行使,提升检察机关司法公信力,确保司法公正真正实现和推进社会主义政治文明建设等社会功能.     

Determination and validation of tetrodotoxin in human whole blood using hydrophilic interaction liquid chromatography-tandem mass spectroscopy and its application

A sensitive analytical method was developed for the quantitative determination of tetrodotoxin (TTX), a powerful sodium channel blocker, in human postmortem whole blood. The sample mixture was cleaned up using cation exchange SPE catridge after protein precipitation by methanol and then separated on a PC-HILIC (phosphorylcholine hydrophilic interaction liquid chromatography) column (150 mm × 2.0mm i.d., 5 μm) using a isocratic elution of 1% acetic acid and acetonitrile. The identification of TTX was performed on tandem mass spectrometry with electrospray ionization interface in positive ion mode. The retention time of voglibose (internal standard) and TTX was 5.1 and 6.0 min, respectively. TTX and internal standard (voglibose) were monitored and quantitated using the ion transitions: the respective precursor to product ion combinations, m/z 320/302 for TTX and m/z 268/92 for voglibose in the multiple reaction monitoring (MRM) mode. The recovery of TTX and voglibose was 61.4% and 62.8%, respectively and the good accuracy (97.7-103.9%), linearity (2-1200 ng/mL) and reproducibility were shown in this method. The limit of detection and limit of quantification were 0.32 ng/mL and 1.08 ng/mL, respectively. This method was applied in the case of three fishermen who were poisoned (including one death) by unknown fish on their boat in October 2010. In this case, the levels of TTX were 27.2, 30.0 and 29.7 ng/mL in heart blood, peripheral blood and serum of a victim, were 3.1 and 12.1 ng/mL in peripheral blood and 3.9 and 12.8 ng/mL in serum of two survivors, respectively.     

硫磺制模法提取雪地足迹的优势

目的比较硫磺制模法与石膏制模法提取雪地足迹的效果。方法分别用两种制模法实验,观察比较操作流程、制模时间、细节特征反映等方面。结果硫磺制模法在雪地足迹提取的速度、细节特征反映等方面优于石膏制模法。结论与传统的石膏制模法相比,硫磺制模法更加迅速、简便,在细节特征的反映更加清晰。     

激光显微切割技术与低体系扩增方法的法医学应用探索

目的建立减少DNA低体积扩增过程中产生气泡的方法。方法采用激光显微切割技术、PALM系统收集目标细胞,并在1μL～1.5μL低体积扩增样本,加入扩增液0.7μL～0.8μL。结果低体积扩增反应中的失败位点比例为2.3%,比常规反应位点时常会产生气泡导致该位点样本扩增失败且达30%以上的比例显著降低。结论低体积扩增方法可以减少或克服扩增过程中气泡的产生,提高扩增成功率。     

内地西藏班(校)学生的跨文化适应

跨文化适应是指身处异文化环境中的个体或群体有意识、有倾向地调整自己的心理活动和行为方式,以便更好地融入异文化的过程,分为社会文化适应和心理适应两个维度。前者以学习适应、生活适应和交往适应为标志;后者以主观幸福感和心理健康为指标,内地西藏班(校)学生的跨文化适应状况是喜少忧多,在诸多方面适应不良。造成这种状况的根源是文化中断。从外部加强社会支持、缩短文化距离,消除歧视与偏见;从内部确立合理的评价与应对方式,增加与文化相关的知识技能,是应对跨文化适应不良的有效措施。