Organic Law 10/2007, of October 8, regulating the police database on identifiers obtained from DNA: historic background and genetic view]

Recently, Basic Law 10/2007 of 8 October has entered into effect, which regulates the police database on identifiers that are obtained from DNA. In the following lines, the author reveals the process of approval of this law as well as approaching certain of its aspects from a genetic perspective.     

Effects of pretrial juror bias,strength of evidence and deliberation process on juror decisions: new validity evidence of the Juror Bias Scale scores

This study examines the validity of the Juror Bias Scale scores in relation to the mock juror decisions reached in two real life homicide cases before and after the deliberation process. The judicial cases used varied in terms of the ambiguity of the evidence presented at both trials. The WLS methodology for statistical modelling of categorical data was used to analyse data. The findings indicated that the Juror Bias Scale scores successfully predict the verdicts and other related questions before and after deliberations in the case with ambiguous evidence. Furthermore, deliberations caused a generalisation effect on the pretrial juror bias in such a case, and enhanced the differences between defense-biased and prosecution-biased jurors in the verdicts delivered after deliberations. The implications of these findings are discussed in relation to the use of pretrial juror bias questionnaires in jury selection.     

Rapid determination of scopolamine in evidence of recreational and predatory use

In recent years, scopolamine has become a drug of common use for recreational and predatory purposes and several ways of administration have been devised. A method for the rapid analysis of suspicious samples was developed, using a portable capillary electrophoresis with contactless conductivity detection. The method allows the separation of scopolamine from atropine which has a similar structure and is present along with scopolamine in some samples. The method was demonstrated to be useful for the fast analysis of several types of evidential items which have recently been reported to have been abused with fatal consequences or employed for criminal purposes. An infusion of Datura stramonium L., in which scopolamine and atropine naturally coexist, was analyzed for being frequently consumed for recreational purposes. A spiked moisturizing cream and six spiked alcoholic beverages were also analyzed. In spite of the complexity of the specimens, the sample pre-treatment methods developed were simple and fast.     

Presupuesto público sin participación ciudadana. La necesidad de un cambio institucional en México para la consolidación democrática

Over the last thirty years, Mexico has undergone an important consolidation of its institutional framework, focused primarily on the electoral and political system. This process of political change was accompanied by a significant transformation and liberalization of the financial and economic system. Mexico’s current situation proves that both processes do not yield satisfactory results when social problems are left unattended. In fact, the transition from a noncompetitive political system to a more democratic one, has not guaranteed better living conditions for most of the population, in part because there is practically no participation in public life on behalf of most of the citizenry. This article reflects on the fundamental importance of citizens’ participation in democracy. Particular attention is paid to public participative budgets as factors which may contribute to democracy’s consolidation and quality, but which have had negligible impacts thus far as is illustrated by public participative budgets initiatives that have been held in Mexico City.     

A business process improvement framework for knowledge-intensive entrepreneurial ventures

Pushed by the transition towards the knowledge economy, as well as several other change drivers, an ever-increasing number of knowledge intensive ventures are relying on operational knowledge intensity in order to generate value. Through their interaction with their varied stakeholders—from actors within their supply chains to educational and financial institutions—knowledge intensive enterprises are increasingly becoming a key component of regional economic stability. Within their complex environment, these organisations lack the support of suitable frameworks to inform their efforts to optimise, adapt and improve their underlying business processes in order to maximise the efficiency of their performance and pursue growth ambitions. This paper examines the distinct nature of knowledge intensive entrepreneurial ventures (KIEs) and the applicability of current business process improvement (BPI) frameworks to their setting. Finally, a KIE-oriented business process improvement framework is developed through an integrative adaptation of the concepts of knowledge intensity and knowledge management to the principles of business process redesign and re-engineering reported in existing literature. The proposed framework contributes to the existing literature in the subject of BPI modelling for knowledge intensive entrepreneurial ventures by addressing a distinct set of improvement concerns that this type of organisations face at a process level.     

Allele frequencies of six miniSTR loci (D10S1248, D14S1434, D22S1045, D4S2364, D2S441 and D1S1677) in a Spanish population

Allele frequencies and forensic parameters for six miniSTR autosomal loci (D10S1248, D14S1434, D22S1045, D4S2364, D2S441 and D1S1677) were obtained from a sample of 264 unrelated individuals from Spain. No significant deviations from Hardy-Weinberg expectations were found. Due to the small PCR products (<125 bp), the use of these non-CODIS (NC) miniSTRs can increase the probability that a degraded sample can be typed. Additionally, these systems can be used in routine paternity analyses where more markers are needed to increase the power of exclusion or in complex paternity cases (e.g. involving closely related individuals).     

Analysis of body fluid mixtures by mtDNA sequencing: An inter-laboratory study of the GEP-ISFG working group

The mitochondrial DNA (mtDNA) working group of the GEP-ISFG (Spanish and Portuguese Group of the International Society for Forensic Genetics) carried out an inter-laboratory exercise consisting of the analysis of mtDNA sequencing patterns in mixed stains (saliva/semen and blood/semen). Mixtures were prepared with saliva or blood from a female donor and three different semen dilutions (pure, 1:10 and 1:20) in order to simulate forensic casework. All labs extracted the DNA by preferential lysis and amplified and sequenced the first mtDNA hypervariable region (HVS-I). Autosomal and Y-STR markers were also analysed in order to compare nuclear and mitochondrial results from the same DNA extracts. A mixed stain prepared using semen from a vasectomized individual was also analysed. The results were reasonably consistent among labs for the first fractions but not for the second ones, for which some laboratories reported contamination problems. In the first fractions, both the female and male haplotypes were generally detected in those samples prepared with undiluted semen. In contrast, most of the mixtures prepared with diluted semen only yielded the female haplotype, suggesting that the mtDNA copy number per cell is smaller in semen than in saliva or blood. Although the detection level of the male component decreased in accordance with the degree of semen dilution, it was found that the loss of signal was not consistently uniform throughout each electropherogram. Moreover, differences between mixtures prepared from different donors and different body fluids were also observed. We conclude that the particular characteristics of each mixed stain can deeply influence the interpretation of the mtDNA evidence in forensic mixtures (leading in some cases to false exclusions). In this sense, the implementation of preliminary tests with the aim of identifying the fluids involved in the mixture is an essential tool. In addition, in order to prevent incorrect conclusions in the interpretation of electropherograms we strongly recommend: (i) the use of additional sequencing primers to confirm the sequencing results and (ii) interpreting the results to the light of the phylogenetic perspective.     

“The big sleep: Elucidating the sequence of events in the first hours of death to determine the postmortem interval”

Recent developments on postmortem interval estimation (PMI) take an advantage of the autolysis process, pointing out to the analysis of the expression of apoptosis and autophagy genes towards this purpose. Oxidative stress plays a role in this signaling as a regulatory mechanism and/or as a consequence of cell death. Additionally, melatonin has been implicated on apoptosis and autophagy signaling, making melatonin a suitable target for PMI determination. The aim of this study was to investigate the early PMI through the analysis of the expression of autophagy genes as well as oxidative stress and melatonin receptor. Our results demonstrated a rapidly increased on the expression of autophagy genes according to the expected sequence of events, then a marked decrease in this expression, matched with the switch to the apoptosis signaling. These results revealed potential candidates to analyze the PMI in the first hours of death, helping to estimate the time-since-death.